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Post by kammy on Jul 16, 2009 12:45:02 GMT -5
Back to the drawing board... what have 'they' done or are trying to do by fusing these bacteria??? Protoplast Protoplast - Wikipedia, the free encyclopedia"Protoplast, from the ancient Greek πρῶτον (first) + verb πλάθω or πλάττω (to mould: deriv. plastic), initially referred to the first organized body of a species. This meaning is similar to the non-biological definition, the first from which all subsequent forms are derived. Subsequently it has several biological definitions: * A protoplast is a plant, bacterial or fungal cell that had its cell wall completely or partially removed using either mechanical or enzymatic means. o Protoplasts - Have their cell wall entirely removed o Spheroplasts - Have their cell wall only partially removed * More generally protoplast refers to that unit of biology which is composed of a cell's nucleus and the surrounding protoplasmic materials."
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Post by kammy on Jul 16, 2009 13:11:21 GMT -5
Protoplast en.wikipedia.org/wiki/ProtoplastUses for protoplastsProtoplasts can be used to study membrane biology, including the uptake of macromolecules and viruses. Protoplasts are widely used for DNA transformation (for making genetically modified organisms), since the cell wall would otherwise block the passage of DNA into the cell.[1] In the case of plant cells, protoplasts may be regenerated into whole plants first by growing into a group of plant cells that develops into a callus and then by regeneration of shoots (caulogenesis) from the callus using plant tissue culture methods.[2] Growth of protoplasts into callus and regeneration of shoots requires the proper balance of plant growth regulators in the tissue culture medium that must be customized for each species of plant. Unlike protoplasts from vascular plants, protoplasts from mosses, such as Physcomitrella patens, do not need phytohormones for regeneration, nor do they form a callus during regeneration. Instead, they regenerate directly into the filamentous protonema mimicking a germinating moss spore.[3] Protoplasts may also be used for plant breeding, using a technique called protoplast fusion. Protoplasts from different species are induced to fuse by using an electric field or a solution of polyethylene glycol. This technique may be used to generate somatic hybrids in tissue culture." **An example of protoplast fusion from plant cells: Fused protoplast (left) with chloroplasts (from a leaf cell) and coloured vacuole (from a petal). Don't we see our 'spheres' - (technical name = protoplasts) coming together like this? So, they are sharing 'information' from one to the other... :eek:
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Post by kammy on Jul 16, 2009 14:38:41 GMT -5
AND... here's the polymer connection: "Different from the procedure for seed plants describe above, fusion of moss protoplasts can be initiated without electric shock but by the use of Polyethylene glycol." Polyethylene glycol Polyethylene glycol - Wikipedia, the free encyclopedia**When you see the letters of a chemical compound front ways and then the opposite - this represents a chain... a like molecule attaches to a like... (as in OH at the beginning and HO at the end, the H's attach to the previous H) as in this example of what Polyethylene glycol is showing: **Our lesions consist of chains of protoplasms that are connected by the fiber networks. Free radicals set this chain in motion thus creating more protoplasms (seeds/specks). (I'm looking at how to stop the OH's from connecting to the HO's... lol). We know that stress is the number one reason for the free radical process to start, also, the antioxidants, (the MSM mentioned) that inactivate free radicals should be used by everyone to slow down the protoplasmic production thus causing less disease. " Clinical usesPolyethylene glycol has a low toxicity[4] and is used in a variety of products. It is the basis of a number of laxatives (e.g. macrogol-containing products such as Movicol and polyethylene glycol 3350, or MiraLax or GlycoLax). It is the basis of many skin creams, as cetomacrogol, and sexual lubricants, frequently combined with glycerin. Whole bowel irrigation (polyethylene glycol with added electrolytes) is used for bowel preparation before surgery or colonoscopy and drug overdoses. It is sold under the brand names GoLYTELY, GlycoLax, Fortrans, TriLyte, Colyte, Halflytely, MiraLAX, and MoviPrep. When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood." **I'm not sure how Polyethylene glycol in these above mentioned products come into play? We've all used laxatives, skin creams, glycerine products... sexual lubricants... hmmm?
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Post by kammy on Jul 16, 2009 15:05:18 GMT -5
I believe we're getting closer to discovering what Morgellons is. We're waiting on Ms. McGyver to receive a fax from her doctor's office so that we can see is PA is also involved. She understood that it was from her conversation with the nurse on the phone, but is not certain.
Ms. McGyver tried to help me with my ear and scalp lesions back in October and contracted Morgellons shortly after contact with me. This is a remote area of Atlanta where this disease is not even heard of by most of our doctors, it is not common. Her culture 'parts' match mine and you that I have photographed.
I'm being treated for PA based on the Petri Dish that I took into the doctor's office, in which, the white growths that both look similar in EC and PA - was thought to be PA by the doctor - from the distinct odor that PA emits. I have yet to have any skin samples cultured and sent to a lab.
We are certain that EC is involved at this time.
I am going to take Ms. McGyver's information to my doctor to get a confirmation that I also have EC at the end of this month. By then, I hope to know enough information on EC (and PA) to help the doctor help me with my treatment. (My ear is in bad shape, I don't have much faith in it being 'saved'.)
I hope there's at least one other person out there that this information helps.
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Post by kammy on Jul 16, 2009 16:30:43 GMT -5
www.insinet.net/jasr/2007/848-852.pdfHere's the only photo that I can find, so far, that shows the microscopic "hybrid" sphere shaped version of E. cloacae. E. cloacae is the one on the left and P. aeruginosa is on the right. E. cloacae has the spots in the centers and P. aeruginosa does not. [/img][/CENTER] Here's one of many comparison photos of what I now believe to be E. cloacae, from human lesion cultures: [/img] [/CENTER] **What's all the hubbub about? Why can't we get treatment from something that is known and for the CDC to make such a mystery of our disease? Well... it might be that all of the EC and PA bacterium are rod-shaped that we might contract from known sources. The sphere-shaped versions are manufactured hybrids from the rods - they are someone's 'creation'. "Protoplast Fusion: The protoplast fusion technique[23] was performed between P. aeruginosa and E. cloacae. The P. aeruginosa and E. cloacae hybrids were selected by using Pseudomonas medium supplemented with L-arabinose, to eliminate P. aerugenosa and incubated at 41oC, to eliminate E. cloacae." Reference: 23. Rajendran, N, E. Sivamani and K. Joiyaraman, 1994. Expression of insecticidal crystal protein gene from a Gram- positive Bacillus expression pattern of the 33- KD a chitinase gene from the mycoparasitic fungus Trichoderma harzianum, Curr. Genet, 28: 478-483.
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Post by kammy on Jul 16, 2009 17:24:34 GMT -5
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Post by kammy on Jul 16, 2009 17:41:42 GMT -5
"Reference: 23. Rajendran, N, E. Sivamani and K. Joiyaraman, 1994. Expression of insecticidal crystal protein gene from a Gram- positive Bacillus expression pattern of the 33- KD a chitinase gene from the mycoparasitic fungus Trichoderma harzianum, Curr. Genet, 28: 478-483."
insecticidal crystal protein... hmmm? Jeany knows what this is....
Have we looked at Trichoderma harzianum?
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Post by kammy on Jul 16, 2009 18:19:51 GMT -5
:eek: US Patent 6926889 - Recombinant bacteria for use in insect control Recombinant bacteria for use in insect control - US Patent 6926889 Claims"Claims 1. A method for delivering and expressing an exogenous gene in a colonial or social insect population comprising: isolating a bacterium endogenous to the colonial or social insect population; transforming said bacterium with a nucleic acid molecule encoding a protein or polypeptide that is exogenous to the colonial or social insect population, wherein the exogenous gene is detrimental to survival of the colonial or social insect population; and introducing said transformed bacterium live into a food source of the colonial or social insect population."
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Post by kammy on Jul 16, 2009 18:47:57 GMT -5
Patent title: CRYSTAL STRUCTURE OF CMY-10, A BETA-LACTAMASE CAUSING ANTIBIOTIC RESISTANCE WITH EXTENDED-SUBSTRATE SPECTRUM CRYSTAL STRUCTURE OF CMY-10, A BETA-LACTAMASE CAUSING ANTIBIOTIC RESISTANCE WITH EXTENDED-SUBSTRATE SPECTRUM - Patent"TECHNICAL FIELD [0001]The present invention relates to a crystal structure of CMY-10 from Enterobacter aerogenes, a β-lactamase of plasmid class C causing resistance for β-lactam antibiotics, more precisely to a method for crystallizing a CMY-10 being a β-lactamase with extended-substrate spectrum, a crystal of CMY-10, and a crystal structure of CMY-10." **CMY-10... ??
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Post by ctbarb on Jul 16, 2009 22:26:25 GMT -5
Kammy, tinyurl.com/n5x7cqUS Patent 6926889 - Recombinant bacteria for use in insect controlUS Patent Issued on August 9, 2005 Estimated Patent Expiration Date: February 1, 2022 Assignee of this Patent is the University of Hawaii and Inventors are: Husseneder, Claudia Grace, J. Kenneth Oishi, Darcy E. Abstract:The present invention relates to recombinant bacteria genetically engineered from insect hosts to express toxic gene products in a pest insect. The present invention also relates to a method of controlling an insect population using such a recombinant bacteria as a delivery agent throughout an insect colony. The invention also relates to a method of delivering and expressing a gene in an insect. CHECK THIS OUT! Excerpts are below...you are right on TARGET! Here are the names of other 'elements' that may be in our cocktail of horror: Exemplary bacteria for use as shuttle host cells include any of the bacteria which occur naturally in a target insect, including, but not limited to, those characterized as follows: Proteobacter, including the genus Pseudomonas; Actinobacter, including Priopionibacterium and Corynebacterium; Firmicutes, including the any species of the genera Mycoplasma, Bacillus, Streptococcus, Staphylococcus; Fibrobacteres; Spirochaetes, including Treponoma and Borrelia; Bacteroides, including the genera Bacteroides and Flavobacterium; and "Termite Group I". Also suitable are any bacteria of the Enterobacteriaceae, including the genus Serratia, including, but not limited to S. marcescens, S. entomophila, S. proteamaculans; any species of Enterobacter, including, but not limited to, E. cloacae, E. aerogenes, E. dissolvens, E. agglomerans, E. hafniae; and any species belonging to the following genera: Citrobacter, Escherichia, Klebsiella, Kluyvera, Panotea, Proteus, Salmonella, Xenorhabdus and Yokenella. This list of horrors have everything that appears to be showing up in our tests, research, and US. Great work!!! I just read this patent the other night.....I need to retrace my steps to see how I got there...I was researching slugs and pesticides, I think....hmmmmm. We're almost there! Jeany and Kammy, you are both top notch researchers IMO...you two are amazing! C'mon folks, let's get the rest of the puzzle together...Game's almost over!!! Hugs, ctbarb
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Post by kammy on Jul 17, 2009 13:41:00 GMT -5
I just called Ms. McGyver (MDR site) to see what her faxed lab results said... and... she says that EC is the only bacteria listed on the report, that PA is not there.
I still believe I am seeing two spheres (protoplasts) in our samples, I believe PA is also involved. I don't know if her lab is set up to recognize a hybrid from of PA? However, until we know more - we have to go with the facts.
Ms. McGyver would come out here and speak for herself... but, she is not too familiar with how to post. In fact, she tried to send me a PM but the system wouldn't let her because she hasn't made 3 posts yet. She is not a computer person, when she comes online - I believe she is reading this thread, though?
Thank you, Ms. McGyver for sharing your information with the rest of us - hopefully, this will give us and our doctors a direction to start looking in.
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Post by toni on Jul 17, 2009 21:04:28 GMT -5
I was reading over a lot of your postings, and just wanted to say (thanks Kammy) for sharing.
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Post by kammy on Jul 17, 2009 22:13:07 GMT -5
Ty, too Toni... here's an interesting place that I ended on a search: Trevor Marshall - Wikipedia, the free encyclopediaTrevor Marshall "According to Marshall, chronic diseases are caused by a variety of bacterial forms including L-form bacteria and biofilm bacteria, which persist and grow in number by interfering with the proper functioning of the innate immune response. Marshall has proposed that chronic diseases can be treated by activating the innate immune response by regular doses of an angiotensin II receptor antagonist (being used as a Vitamin D Receptor agonist).[1][2] Patients also take pulsed low doses of bacteriostatic antibiotics and avoid consumption of Vitamin D. A 2009 paper by Perez stated that the Marshall Protocol has been used successfully to treat many chronic conditions, including lupus, rheumatoid arthritis, scleroderma, sarcoidosis, Sjögren's syndrome, autoimmune thyroid disease, psoriasis, ankylosing spondylitis, Reiter’s syndrome, type I and II diabetes mellitus and uveitis.[citation needed] However, one pair of dermatologists have stated that the ability of this Protocol to treat sarcoidosis is only supported by anecdotal data and that it cannot be recommended to patients.[3]"
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Post by toni on Jul 18, 2009 8:23:13 GMT -5
There's a gal (Speculation) who use to post alot about this subject too - the Vit D/Marshall protocol. Maybe you can search her posts for more on this, as she did post alot of good info.
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