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Post by bannanny on Nov 24, 2009 22:29:36 GMT -5
Not to worry jh... I think we have enough cultures going in now to be able to find something out for all of us! I sent mine yesterday AM via overnight mail (which was another 17.00) but still worth every penney! So Dr. Abbott should've received it today. He sent the email above yesterday so he hadn't received it yet. But it should be there by now!
huggies ~~ bannanny
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Post by bannanny on Nov 24, 2009 23:43:59 GMT -5
I just wanted to say there's 3 more people who will be sending in cultures to Dr. Abbott...
our gertie girl, jody, and ivanhole. Jody called me and said she hasn't had a computer for 2 months now is why she hasn't been on the board. I told her about the cultures and all, so she asked for Dr. Abbott's number and address and said she'd go ahead and do it now too. I called gertie and told her and she's already received her kit from the good doctor. I'd mentioned it to ivan on his thread as well and wanted him to get involved too. He pm'd me and got all the info as well! So it looks like we're gonna be keepin the good doctor busy for awhile!!
hugs ~~ bannanny
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Post by Sidney on Nov 24, 2009 23:53:25 GMT -5
Thanks so much, Bananny. It would be very helpful if lots of folks participated in this opportunity. I feel especially encouraged regarding your post about Dr. Abbott's interest in Vector Borne diseases and that they can also culture bacteria.
You definitely picked a winner. Thank you!
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Post by mercury on Nov 25, 2009 3:58:10 GMT -5
Hey Bannanny, Ruth, Sid etal. Thanks so much for driving the getting cultures effort forward. I will we sending for petri dishes this week and will get them back to the lab ASAP.
Lots of holiday cheer (if that's possible) to all.
Merc
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Post by ruth on Nov 25, 2009 13:36:07 GMT -5
i posted on the other thread,
i am holding my sterile dish to see if i get the same growth. it is not as before.
the media is malt with chloramphenicol. (we have all had trouble getting our stuff cultured)
it appears the type of media used to culture is very important. its' like taking the wrong abx.
at 12pm i should have 24 hour growth. it was easily ascertained using the liquid media that came with the pro-lab culture dish.
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Post by ruth on Nov 25, 2009 14:17:26 GMT -5
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Post by Sidney on Nov 25, 2009 14:34:45 GMT -5
I hav e no idea what Tryptophan is...do you? Yes, it appears to be the same PRO-LAB whose petri dishes we recently used.
I do not have time to check it, but my memory tells me tryptophan has to do with turkeys....
I had to enlarge the print to 200% to read it, but I'm sure it is the same lab.
So, are you thinking we're getting false positives from these culture kits?
I'm having my biopsy and skin scrapings next Tuesday at 10 :45. Will take everything I need to get the dish mailed to Dr. Abbott immediately including the Express Mail envelope which I'll address before the appointment..
I will not be touching the petri dish so there's no way anyone can say I "seeded" the growing medium.
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Post by ruth on Nov 25, 2009 16:55:16 GMT -5
if no growth appears in the 'natural link lab' petri dish
then i am going to buy another pro-lab kit as i know it will culture Morgellons.............
i will send it into dr. abbott for the identification as he has said he would accept those cultures and he is the expert in the field of identification.
i absolutely do not want a negative, which is what all the professionals who have cultured my stuff said.
it is the wrong culturing media if i do not get growth by tomorrow morning. i would be devastated sending in a culture that doesn't go anywhere.
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Post by Sidney on Nov 25, 2009 17:11:27 GMT -5
I would be devestated too, Ruth. (Totally and Off the Charts devestated.)
My understanding has always been that SPECIFIC SPECIES of molds/fungus require a SPECIFIC culture medium. It's a case, as I understand it, of One Size Does Not Fit All.
It's also my understanding that typically it should take around four weeks to culture fungus. (All molds are fungus, but not all fungi are mold.)
Yet, we all so easily grew Mold with the Pro-Lab kits. Like you, I am concerned, but I haven't tried culturing anything in the petri dishes from Dr. Abbot's lab.
I will say that I have read one of the papers that came with the petri dishes and his credentials are right up there with the very best there is. As an example, the State of Texas uses his lab, yet Texas has some outstanding mycologists in their own home state. (Dr. Abbot is in Reno.)
So, as you mentioned, we can't give up on this. I have one Pro-Lab kit left, and if necessary I will do this entire process over again and send it to Dr. Abbott.
Those plastic looking facial hairs I've been screaming about all these years often have white bumps growing on them from root to end. Maybe it's White Piedra, maybe something else, but absolutely CANNOT be anything close to normal.
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Post by bannanny on Nov 25, 2009 17:42:15 GMT -5
I got this email from Dr. Abbott today and want to post it on all the threads pertaining to the cultures cuz it's very important we all know this info... Hi Robin:
We received your sample this morning in the mail, it arrived safely. That was expensive by priority mail, there are some other options for you... Our clients in California often use the courier OnTrac (formerly California Overnight), which typically costs less than $15 for overnight delivery, or perhaps a two-day delivery by Priority Mail or Fed Ex would cut costs and still get the samples to the lab fairly quickly. Also, I would recommend that petri dishes be sent in a small box (couriers and USPS provide free boxes), to better protect the fragile plates. This one in bubble envelope was fine, but we have had some crushed plates in the past... I am setting up your account in the data base and need a few more bits of info including your phone number and shipping address (courier needs physical address not PO box) if we send you supplies. Also, I have attached our Chain of Custody form which should be filled out for each project/sample sent in to the lab (sorry, I forgot to send this before, I filled one out for you this time). Do you have info on when this sample was taken (date of sampling)? Also, we usually give each project a name and for yours I will simply call it ‘Mold 112509’ if it is appropriate to use the date received. Thanks! Talk to you again soon.
SeanHere's the chain of custody form altho it's a pdf file so it didn't copy quite right. But you can see the info he needs anyway.... Account name Sampling date Submitter Project / P.O. Phone FME NFME FC BC EC 24hr 48hr Submitter's Date Receiver's Date Signature Time Signature Time Submitter's Date Receiver's Date Signature Time Signature Time Page ___ of ___ Natural Link MOLD LAB, Inc. is a Nevada Corporation (v 4.0) © 2004 Sample identification, description, and/or location Sample volume : am pm Analysis * Alternative / additional analysis requested: RUSH Lab use: Control #: (*) FME, Fungal Microscopic Examination -- NFME, Non-Fungal Microscopic Exam -- FC, Fungal Culture -- BC, Bacterial Culture -- EC, E.coli (coliforms) ID ____/____/____ ____/____/____ : am pm : am pm ____/____/____ : am pm ____/____/____ Natural Link MOLD LAB 4900 Mill Street Suite 3 Reno, NV 89502 (866) 252-6653 (866) 252-MOLD Phone (775) 356-6653 Fax (775) 356-6639 info@naturallinkmoldlab.com Chain-of-Custody FormIf you guys have any questions or want to ask him about your concerns with the pro lab kits, just call or email him. He's happy to help! hugs ~~ bannanny
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Post by Sidney on Nov 25, 2009 20:46:52 GMT -5
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Post by ruth on Nov 26, 2009 11:31:56 GMT -5
i have growth this morning in my control dish. happy thanksgiving ;D
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Post by bannanny on Nov 26, 2009 16:27:10 GMT -5
Dr. Abbott replied to me about your concerns ruth. Here ya go! Hi Robin:
They do not list the ingredients and simply call it Mold Growth Medium (it is not an agar-based medium). In my experience it is adequate to isolate most fungi, but not the best medium and I would recommend our Malt extract agar plates (MEA), which are routinely used for isolation of a wide variety of molds and other fungi. Any fungi that are not growing well on the routine media will be transferred to specialized media for species identification in the lab. Take care.
SeanYep... it's gonna be a good year! love you ~~ bannanny
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Post by ruth on Nov 27, 2009 1:43:47 GMT -5
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Post by kammy on Nov 27, 2009 11:03:34 GMT -5
Hi Guys... Ruth...
Jeany and I are looking at these photos above and they look different than anything we've seen so far. These are very thick 'hyphae' not like the others... just saying...
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Post by jeany on Nov 27, 2009 11:15:01 GMT -5
WOW! Awesome slide show, Ruth! Can't help it, but it looks rather like branching roots (rhizomes) with sclerotia to me..so I looked it up and found this: Charcoal rot - Macrophomina phaseolinaplantpath.caes.uga.edu/extension/plants/fieldcrops/CornCharcoalRot.htmlDisease Development and Spread Very little is known regarding this disease on strawberries. M. phaseolina is a common soilborne pathogen in many warm areas of the world and has a very broad host range. Many vegetable crops planted as second crops after strawberry such as squash, cantaloupe, and peppers, legumes and others are susceptible. Those infections may increase inoculum levels of M. phaseolina in the soil in the off season for strawberries. In general, high temperatures and low soil moisture favor infection and disease development. edis.ifas.ufl.edu/pp161Macrophomina phaseolina (Tassi) Goid. a soilborne fungus causes charcoal rot. The fungus can infect the root and lower stem of over 500 plant species and is widely distributed in the United States (8). Charcoal rot is an important disease during hot, dry weather or when unfavorable environmental conditions stress the plant. M. phaseolina causes disease on soybean, peanut, and corn. In peanut, it causes seed and seedling rots, wilt, root and stem rots, leaf spot, and rotting of developing pods and seed. Charcoal rot on soybean leads to early maturation, chlorosis and incomplete pod filling. While in corn the fungus causes a stalk rot during hot, dry conditions. www.cals.ncsu.edu/course/pp728/Macrophomina/macrophominia_phaseolinia.HTMLook at the pic here on this site.. www.agroatlas.ru/en/content/diseases/Gossypii/Gossypii_Macrophomina_phaseolina/.....(can't insert it) The causative agent of Charcoal Root Rot of Cotton is the fungus Macrophomina phaseolina developing during its life cycle in anamorphous stage; black pycnidia are formed under epidermis. The sterile mycelium stage (Rhizoctonia bataticola) is also present in the cycle of its development. Morphological structures of the pathogen include conidia (pycnospores) on very short threadlike conidiophores, septate vegetative mycelium, and black (charcoal) fine sclerotia, 50 mkm to 1 mm long. The disease can appear on cotton at its early development stages, but its symptoms basically accrues right after flowering and later in form of radical or root rots. Deep necrosis appears on the affected parts, reaching vascular system and central tissues. The root tissue lags behind or falls off as a result. Numerous fine sclerotia appear on affected tissue, which appears strewed with coal. At the disease intensive development, sudden withering of cotton plants occurs, but the leaves do not fall down for a long time. Fungus M. phaseolina affects many field cultures and wild plants. Sources of the pathogen infection are sclerotia in soil and affected vegetation residues and weeds. Jeany
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Post by jeany on Nov 27, 2009 11:27:20 GMT -5
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Post by bannanny on Nov 27, 2009 18:23:40 GMT -5
Wow... awesome pics ruth! I just have to repeat something I just heard on Bonnie Hunt (I just love that woman!) She said when she was going thru some really hard times during high school and asked her father (when he was still alive) "why me dad?" He said to her... "It's people with character and love inside who God gives the most challenges to... you must consider it a compliment." I love you guys ~~ bannanny
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Post by bannanny on Nov 27, 2009 18:34:25 GMT -5
Hey ruthie... do you want me to send your slideshow to Dr. Abbott? If you do, tell me the mag you took it at ok? love ya ~~ bannanny
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Post by ruth on Nov 27, 2009 21:24:31 GMT -5
bananny, i don't know what you mean, "what mag did you took it at"?? i got the culture sent in to abbott today. the thicker the mold gets the harder it is to see how long the strands grow on the base. it must grow on top of each other till it is a solid mat of growth. it becomes as a blur..........many of us have seen the blur above and around artifacts. only at the edges can you see the strands. this is probably the reason dr. t. to send them in right away so he can see the growth. dr. abbott can always just take some out of one dish and culture another dish. it will grow the same stuff. on the previous culture.......i thought i saw the growth as the images in slide show.........then the zygomycetes looking was everywhere. i'm going to go see, but since it was not done using sterile technique, it doesn't mean as much. whether this recent culture will mature and it will be as the other culture? i'll be checking and posting images. i just gotsta know we are on a weekend and i didn't tell them priority mail. i was thinking by using their box it is priority, but now i am not sure how it goes. it was way cheap under $5. i don't know how i could forget that part.
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