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Post by jeany on Nov 20, 2009 13:35:06 GMT -5
While getting another cup of coffee..hehe..Kammy mentioned something to me about a fungus on grapes, strawberries.. We need to look what fungus that could be, she said..she's the 'brain' folks..I'm only her assistant.. ;D..so I went back to my pc and did a little research.... Here it is what we think..Botrytis cinerea It's a common fungus on grapes, strawberries and other fruits..and also vegetables such as tomatoes, carrots and lettuce. It starts out as a whitish cottony like looking fungus and then turns green and then dark grey. Botrytis cinereaen.wikipedia.org/wiki/Botrytis_cinereaBotrytis cinerea is a necrotrophic fungus that affects many plant species, although its most notable hosts may be wine grapes. Here's a pic of BC: Botrytis cinerea is characterized by abundant hyaline conida (asexual spores) borne on grey, branching tree-like conidiophores. The fungus also produces highly resistant sclerotia as survival structures in older cultures. It overwinters as sclerotia or intact mycelia, both of which germinate in spring to produce conidiophores. The conidia are dispersed by wind and rain-water and cause new infections.** just like Frito said today. It 'grew' with water contact!! Botrytis cinerea mold on grapes may cause "winegrower's lung", a rare form of hypersensitivity pneumonitis (a respiratory allergic reaction in predisposed individuals). ** is this fungus what is causing our sinus infections, our draining eyes, lost of vision, ear infections, coughing...? Another aspect I found interesting is that this fungus grows respectively reproduces differently within seasons. This picture lets me think that perhaps our 'black and white specks' might be sclerotia and that we have both sclerotia and conidia depending on it's development stage. Here take a closer look at sclerotia: ** I think the first picture shows exactly what Frito showed today. And the second one shows the 'spheres' we've been looking at! The third picture looks definitely 'morg like' to me. And look at this picture, Frito. Doesn't it look like yours? The one that showed those round spheres with a blue ring around them and something in it? AND..LOOK at this picture. WOW..I thought! That looks exactly like our ribbon like twisted fibers! And the black speck to the left..just like in Frito's pic! And this one even shows the 'bulbs' we've been seeing in Kammy's pix? What do you guys think? Jeany
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Post by kammy on Nov 20, 2009 20:41:49 GMT -5
That is not true, Jeany - we are all smart here and have something to offer. I've already determined that Frito and Jeany are smarter than me... and a few others out here, too. We're all unique and have a place in this, this is a piece of work shared by all of us participating, and we all should be very proud of what we're trying to accomplish for ourselves, each other and humanity.
I think it's good to speculate at this time, because... if we have predetermined answers based on what we believe we're understanding at this time and we get back solid data and it doesn't meet it - then we can have our doctors look into this aspect closer to see what it is that one of us has missed. This is the most information sharing we've done amongst each other so far, I don't know about you, but I'm learning something daily. Eventually, when we get our reports back, we will know more truths and then we can eliminate some of our theories, if they have missed the mark.
I think we need to look closer at strawberries for sure, and possibly - tomatoes, carrots and lettuce and research Botrytis cinerea in more depth.
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Post by kammy on Nov 20, 2009 21:03:20 GMT -5
I KNOW we have more than one 'thing' happening... it's not as simple as us being allergic to Rhizopus... our numbers would be a lot larger. There's several agents at work, what are they? I had a question about which fungi inhabits dogs, cats, mice and humans and came across this one just now... and viewing the images... it looks close, also... it needs to be checked out - Cryptococcus neoformans my-stuff-dot-com.com/LB/Cryptococcus neoformans.jpg[/img] I have seen one of our spheres reproduce like this below: my-stuff-dot-com.com/LB/Cryptococcus neoformans 2.jpg[/img] What's the best way to do this, Frito? To look at what we know to be true with our sphere photos ... and match them with our suspects?
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Post by jeany on Nov 20, 2009 21:30:56 GMT -5
I KNOW we have more than one 'thing' happening... it's not as simple as us being allergic to Rhizopus... our numbers would be a lot larger. There's several agents at work, what are they? I had a question about which fungi inhabits dogs, cats, mice and humans and came across this one just now... and viewing the images... it looks close, also... it needs to be checked out - Cryptococcus neoformans my-stuff-dot-com.com/LB/Cryptococcus neoformans.jpg [/img] I have seen one of our spheres reproduce like this below: my-stuff-dot-com.com/LB/Cryptococcus neoformans 2.jpg[/img] What's the best way to do this, Frito? To look at what we know to be true with our sphere photos ... and match them with our suspects?[/quote] Yes, it sure does look suspicous, Kam..wasn't there research on this board months ago (Feb./March) according this fungus, if I recall right? hmmm... Jeany
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Post by kammy on Nov 20, 2009 21:32:53 GMT -5
I'm still just looking at the images for Cryptcoccus... I haven't looked at the reference material yet - and do I see an occluded, infected sphere, such as I've been describing with the baculovirus? This guy looks very suspicious!: Human sample, 100x: [/img][/center]
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Post by kammy on Nov 20, 2009 22:17:29 GMT -5
Yes, it sure does look suspicous, Kam..wasn't there research on this board months ago (Feb./March) according this fungus, if I recall right? hmmm... Jeany I don't know, Jeany, if someone else has already brought up Cryptococcus neoformans? I know I've looked at it before but didn't start using Google images until recently and one of our spheres is crypto-like, enough for me to identify it initially as the Cryptosporidium bacteria... the sphere is reddish at times like in the Cryptosporidium images. Human sample, 100x:
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Post by kammy on Nov 20, 2009 22:50:40 GMT -5
What Makes Cryptococcus neoformans a Pathogen? tinyurl.com/ylgjg8r"Life-threatening infections caused by the encapsulated fungal pathogen Cryptococcus neoformans have been increasing steadily over the past 10 years because of the onset of AIDS and the expanded use of immunosuppressive drugs. Intricate host-organism interactions make the full understanding of pathogenicity and virulence of C. neoformans difficult. We discuss the current knowledge of the characteristics C. neoformans must possess to enter the host and establish progressive disease: basic growth requirements and virulence factors, such as the polysaccharide capsule; shed products of the organism; melanin production; mannitol secretion; superoxide dismutase; proteases; and phospholipases. Cryptococcus neoformans is an encapsulated fungal organism (Figure 1) that can cause disease in apparently immunocompetent, as well as immunocompromised, hosts (1,2). Most susceptible to infection are patients with T-cell deficiencies (1,2). C. neoformans var. neoformans causes most cryptococcal infections in humans, so this review will focus on information from the neoformans variety of this basidiomycetous fungus. C. neoformans var. neoformans is found worldwide; its main habitats are debris around pigeon roosts and soil contaminated with decaying pigeon or chicken droppings (1,3). Not part of the normal microbial flora of humans, C. neoformans is only transiently isolated from persons with no pathologic features (2,4). It is generally accepted that the organism enters the host by the respiratory route in the form of a dehydrated haploid yeast or as basidiospores. After some time in the lungs, the organism hematogenously spreads to extrapulmonary tissues; since it has a predilection for the brain, infected persons usually contract meningoencephalitis (1). If untreated, cryptococcal meningoencephalitis is 100% fatal, and even when treated with the most effective antifungal drugs, cryptococcal infections can be fatal if the host does not have adequate T-celldependent immune function (2)." my-stuff-dot-com.com/LB/Cryptococcus laurentii.jpg[/img]
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Post by kammy on Nov 20, 2009 23:10:25 GMT -5
Now, if you look - you will see that Cryptococcus laurentii or neoformans will tie in with Botrytis cinerea. There appears to be something similar to a combination of these fungal pathogens mentioned happening possibly along with a Rhizopus allergy or toxic buildup. tinyurl.com/ylmfxslWhat do we know for sure - 1. We are now able to microscopically identify the main Morgellons 'artifacts'. 2. These pathogens, from what we can see - appear to be mostly fungal or mold in nature. 3. The same fungal spore is appearing in all of us. 4. We believe that these same pathogens can be found when red wine, cigarette filter cotton, bathroom tissue, some areas tap water and q-tips are cultured in a Petri Dish. 5. We can culture these same artifacts from our blood, lesion debris, urine, tears, nasal secretions, saliva... Our pathogen is in red wine and tap water!... It has to be something considered safe? tinyurl.com/yf99x28"Kiss my Aspergillus pusware.com/rdct/?tag=fungusAspergillus is everywhere. It can be the mold on your bread, the mold on your cheese, and the mold on the cup of coffee that is left out for three or four weeks. My wife, and most females, have curiously never seen mold on a cup of coffee left out for weeks. Most males have. Don’t know why, but when I was single I would let the dishes sit in the sink until the growth evolved into a life form that wanted to clean itself. I don’t do that anymore. So when aspergillus is in a bronch culture, you have to ask if it as real or a contaminant, and Ella Fitzgerald can’t help you with that. The patient has had Wegeners for 5 months and is on dialysis, prednisone, and just finished the Cytoxan that cleared up the pulmonary inflitrates on her CT. Now back in the hospital, she has cavitating pulmonary nodules, the BAL from the bronchoscopy is growing aspergillus after only three days. She is a touch short of breath, but no hemoptysis. The gold standard for invasive aspergillus is biopsy showing invasion of the organisms, but the bronch biopsy did not show this. Open lung? Treat? The BAL had aspergillus on staining, so it must be a fairly high concentrations and she had no prior structural lung disease to predispose to heavy colonization. The chance that the aspergillus is the real deal is about 25%. Not good odds. But it’s a compatible CT and host and large amounts of aspergillus that grew rapidly. If the galactomannan assay is positive, then the diagnosis is clinched. So she is on voriconazole and we shall see. Thats the royal we, not the multiple personality we. ======= Mayo Clin Proc. 1992 Mar;67(3):221-7.L Bronchoalveolar lavage (BAL) has been used extensively for assessment of immunocompromised hosts with pulmonary infiltrates. Reported estimates of the diagnostic utility of BAL have varied because of differences in patient populations, diagnostic criteria, and study methods. Herein we report on the use of BAL to determine at least one of the final diagnoses in 150 immunocompromised patients. Although the frequency with which BAL provided at least one of the final diagnoses (overall diagnostic yield) was seemingly low (39%), the yield increased substantially when only patients with pathologically proven diagnoses were considered. The sensitivity of BAL was 82%, and the specificity was 53%. The use of rigid diagnostic criteria enabled us to distinguish pathogens from colonizers. Pneumocystis was considered a pathogen whenever it was identified. It was the most common infectious pathogen identified (50%) despite the fact that our study population had relatively few patients (only 4%) with acquired immunodeficiency syndrome (AIDS). Organisms such as cytomegalovirus, Aspergillus, and Candida were frequently identified in BAL specimens but were eventually proved to be pathogens in only 24%, 25%, and 0% of cases, respectively. BAL detected pulmonary malignant lesions on the basis of positive cytologic results in four of six patients eventually found to have primary or metastatic lung cancer. Our results should enhance the understanding of the strengths and weaknesses of BAL and assist in the interpretation of associated microbiologic findings."
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Post by kammy on Nov 20, 2009 23:49:47 GMT -5
PHENOTYPIC TRAITS OF WOUND COMPETENCE OF POSTHARVEST BIOCONTROL YEASTS AND POTENTIAL OF THESE MICROORGANISMS FOR PREVENTION/ DETOXIFICATION OF MYCOTOXIN ACCUMULATION www.actahort.org/members/showpdf?booknrarnr=682_293Abstract: "Knowledge of the mechanisms of action of biocontrol agents (BCAs) is crucial to identify underlying pivotal traits, whose enhancement could boost antagonist activity and encourage the use of BCAs as alternative/integration of fungicides. We had previously shown that resistance of postharvest BCAs to oxidative stress is a possible key mechanism for their wound competence to successfully outcompete wound pathogens in stored apples. In this work we describe recent studies aimed at elucidating the phenotypic traits underlying wound competence of postharvest biocontrol yeasts. Further, we show that some BCAs display a potential activity in addressing other major issues of food safety: contamination of apple-based food products with Patulin and of wine with Ochratoxin A, mycotoxins produced by the fungal pathogens of apples and grape Penicillium expansum and Aspergillus carbonarius, respectively. " Patulinen.wikipedia.org/wiki/Patulin"Patulin is a mycotoxin produced by a variety of molds, particularly Aspergillus and Penicillium. It is commonly found in rotting apples, and the amount of patulin in apple products is generally viewed as a measure of the quality of the apples used in production. It is not a particularly potent toxin, but a number of studies have shown that it is genotoxic, which has led to some theories that it may be a carcinogen, though animal studies have remained inconclusive.[2] Patulin is also an antibiotic.[1] Several countries have instituted patulin restrictions in apple products. The World Health Organization recommends a maximum concentration of 50 µg/L in apple juice.[3] In European Union the limit is set to 50 micrograms per kilogram (mcg/kg) in both apple juice and cider, and to half of that concentration, namely 25mcg/kg in solid apple products and 10mcg/kg in products for infants and young children. These limits came into force on 1 November 2003." Ochratoxin Aen.wikipedia.org/wiki/Ochratoxin_A"Ochratoxin A, a toxin produced by Aspergillus ochraceus and Penicillium verrucosum, is one of the most abundant food-contaminating mycotoxins in the world.[1] Human exposure occurs mainly through consumption of improperly stored food products,[2] particularly contaminated grain and pork products, as well as coffee[3], wine grapes [4] and dried grapes. The toxin has been found in the tissues and organs of animals, including human blood and breast milk.[5] Ochratoxin A toxicity has large species- and sex-specific differences.[3]"
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Post by kammy on Nov 21, 2009 0:15:53 GMT -5
This photo right here, Frito... This is after you mashed the spore and what was inside - 'Fibers'?... we saw in my cigarette filter experiment where the fibers can create the fungus in sphere form, then showed this sphere exploding and showed what appears to be 'our' fungus. The beginning of the life cycle of our fungus can be in the form of a thread fiber, such a sewing thread - such as Barb had and we cultured which also created the various Morgellons artifacts. It can be in the form of wood/paper fibers, as we saw with the bathroom tissue, it can be in the form of cotton fibers - such as in the q-tip. It can be in the form of what's inside your sphere. The fibers are like a male and female - they are female in that they 'birth' the spheres... they are male-like in that they fertilize them once they are created. Or, it is possible that they are one of each, they are known as filamentous fungi.
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Post by kammy on Nov 21, 2009 5:14:47 GMT -5
Filamentous Fungi They say our fibers aren't the same as filamentous fungi - exactly - I think we have some sort of souped-up version, just coat them with polymers or gel... anyway, I do believe one scientist found fungi in their makeup? www.springerlink.com/content/m7215959414w8q54/Abstract: Filamentous fungi are commonly used in the fermentation industry for large scale production of glycoproteins. Several of these proteins can be produced in concentrations up to 20–40 g per litre. The production of heterologous glycoproteins is at least one or two orders of magnitude lower but research is in progress to increase the production levels. In the past years the structure of protein-linked carbohydrates of a number of fungal proteins has been elucidated, showing the presence of oligo-mannosidic and high-mannose chains, sometimes with typical fungal modifications. A start has been made to engineer the glycosylation pathway in filamentous fungi to obtain strains that show a more mammalian-like type of glycosylation. This mini review aims to cover the current knowledge of glycosylation in filamentous fungi, and to show the possibilities to produce glycoproteins with these organisms with a more mammalian-like type of glycosylation for research purposes or pharmaceutical applications. Heterologousen.wikipedia.org/wiki/Heterologous"In cell biology and protein biochemistry, heterologous expression means that a protein is experimentally put into a cell that does not normally make (i.e., express) that protein.[1] Heterologous (meaning 'derived from a different organism') refers to the fact that often the transferred protein was initially cloned from or derived from a different cell type or a different species from the recipient. Typically the protein itself is not transferred, but instead the 'correctly edited'[2] genetic material coding for the protein (the complementary DNA or cDNA) is added to the recipient cell. The genetic material that is transferred typically must be within a format that encourages the recipient cell to express the cDNA as a protein (i.e., it is put in an expression vector). Methods for transferring foreign genetic material into a recipient cell include transfection and transduction. The choice of recipient cell type is often based on an experimental need to examine the protein's function in detail, and the most prevalent recipients, known as heterologous expression systems, are chosen usually because they are easy to transfer DNA into or because they allow for a simpler assessment of the protein's function." Glycoprotein en.wikipedia.org/wiki/Glycoprotein
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Post by toni on Nov 21, 2009 9:06:06 GMT -5
I KNOW we have more than one 'thing' happening... it's not as simple as us being allergic to Rhizopus... our numbers would be a lot larger. There's several agents at work, what are they? I had a question about which fungi inhabits dogs, cats, mice and humans and came across this one just now... and viewing the images... it looks close, also... it needs to be checked out - Cryptococcus neoformans my-stuff-dot-com.com/LB/Cryptococcus neoformans.jpg [/img] I have seen one of our spheres reproduce like this below: my-stuff-dot-com.com/LB/Cryptococcus neoformans 2.jpg[/img] What's the best way to do this, Frito? To look at what we know to be true with our sphere photos ... and match them with our suspects?[/quote] --------------------------------------------------------- Kammy, I sure agree, (we've got the cocktail) of not only fungi, but bacteriums too! Question has remained with me- HOW did we get so many "all at once"? That's what leaned me towards GMO to begin with. (a few years ago two different people scanned their blood tests results) and they were loaded with 12-15 different bacteriums too. That blew me away when I saw that each and every one of them were 'used specifically' in genetic engineering...because they weren't common bacteriums either. And something SO odd this morning, hahah when I typed in Lyme Busters (in google) to log on, the words "C. Neoformans" hit me, and then I come to the board and see you here talking about this. Wow...I've been having so many "same sames" or coincidences going on, I can't even say, it's so unreal. Every day, it's going on. I must be "vibing" I call it....really strong lately. Not that this has anything to do with anything, but it's just so bizarre.
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Post by fritolay66 on Nov 21, 2009 9:24:46 GMT -5
No Kammy.
That photo is not of what was inside. That photo shows the hairs on the outside. There was another photo with that one, and showed where the hairs were attatched. These were on the outside. I did not "mash" the specimen so that its contents on the inside could be seen. I only "mashed" it enough to turn it and see if it was squishy. It was not.
Frito
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Post by kammy on Nov 21, 2009 12:30:56 GMT -5
The hairs I told you about. These could be seen after I tentatively squished the specimen. I couldn't see them prior to "squishing" the sample. 100 mag Frito This is what it sounds like from your description, Frito - sorry for misunderstanding it. Anyway, we know there are fibers inside the spheres, we can see them sticking out, that is my point. Just trying to get us to take notice of what these spheres and fibers are doing, their life cycle, I'm asking for your observations to back mine up - you must be totally unbiased and say what you think. I'm seeing the spheres are like a womb 'egg'/female - the fibers inside are like sperm/male... but, there are obviously female fibers that create the spheres inside there too... (red - male / blue - female) I'm wondering if some aren't both, that was where I was going up there, that we need to start watching for this aspect. I just showed in the cigarette filter fiber experiment that all that is needed to create some of the Morgellons artifacts starts with a fiber. Whether or not the future polyhedron appear as they do in human samples is yet to be seen, I suspect they do. (I will photograph this dish and report soon on whether they do or not.) IF... a fiber from a cigarette filter can start the Morgellons artifacts growing - we might think that a fiber inside of us, which appears to be the same, could also start the life cycle process that is our disease and a fiber could possibly 'infect' others if the conditions were right? HAS ANY SCIENTIST CULTURED A MORGELLONS FIBER, YET? The reason I haven't done it is because they are small and when mine come out of my ear, there's other debris with them - making for a 'contaminated' experiment. A single fiber needs to be in the Dish by itself for starters. And, which might lead us to think - that IF a person can remove the fibers - they will remove the specks/spheres... and not have lesions. ?? (I will "mash" one at some time and see what all comes out... I think Toni has done this experiment already, maybe she can tell us what she saw?)
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Post by toni on Nov 21, 2009 12:47:25 GMT -5
Kammy, Do you mean when I mash specimens? See...(this is one of those imho things) cause all I have to go by is 'what I have seen' over and over with my specimens. I can take a tiny little specimen ( either by having that specimen) on the tip of my needle, or a wooden toothpick, and my hair will follow that specimen. I've seen multi-colored incredibly fine fibers wrapped amongst my specimens, on the outside, or inside of them. What that "is to me"...is my specimens, by the way my hair will follow them, and by the way "my colored clothing" super fine fibers are stuck to them, (imho) tells me "there's a magnetic component to the "specimens"...whether they be of some type of substance mixed within 'our goo stuff' that expresses from our skin, then hardens, or if it's a magnetic component to the 'fungi'. I don't know though for certain, anything really...just what I see. I think the "clear fibers though" - not all...but some, is hyphae growth, from some kind of fungi...or many different kinds within us. But "this gel stuff" or whatever it is, that is "contaminating" us ...which again is only a (imho) thing....is magnetic. I've got no doubts (imho). I think that too is why "particles" will hover before me. I've seen that happen too many times, and they hover by my face, which I "believe" is because my face is loaded with some type of magnetic substance, along with all the bacteriums and fungi. Heck...maybe the magnoteto (sp?) bacterium is creating that part...I don't know. I also have blanked out on how to spell that particular bacterium (something like mageteto)? geeze
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Post by toni on Nov 21, 2009 12:52:09 GMT -5
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Post by rhorn2006 on Nov 21, 2009 18:01:33 GMT -5
Poor Kammy!!! From the pics Frito posted,, poor Kammy has got this stuff really bad!! I dont think I want a closer look at my stuff now!!
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Post by fritolay66 on Nov 21, 2009 22:37:57 GMT -5
Forgive me guys as I am alittle behind here.
Kammy!!
I couldn't wait to get back here. Last night before I passed out, I had another speck from my tear duct, so again I added water to it. I will try to explain this better. Again, the entire thing enlarged. There were two puffy type areas and each had a fiber. The "sewing thread" type. With the addition of water, as the puffy area was getting puffier, I watched that thread penetrate that area and disappear into it. The other area did the same thing. While pentrating, the entire sample moved. That thread moved the sample. When it went in this puffy area, lets call it a sac, the thread could no longer be seen. Weird huh?
I have pictures, and will post but I haven't uploaded them as of yet.
Okay, now lets go back to the first sample in which I added water and posted here. That water dried on the slide, so I decided to have a look see to see if anything transpired in the drying. The fibers have growth on them. I don't know about you, but it looks like conidia. The sample in which grew is a smear of nothing really interesting. The fibers though, now they were interesting.
I couldn't wait to tell you about them, I will upload those pics tonight and have them posted for you ASAP. I was thinking that the threads were the reproductive part and I also thought they were the beginning of the process too. Geez, after what I watched and then the pictures of the dried slide from the first experiment, I do still believe those fibers are the beginning. I concur.
This will be my next goal. I will try to have all this done by the am.
Yes, okay. then I agree with your point.
Kmarie!!! I am so happy to see you around. And Toni. Thank you both, I have poured all over spirochettes, and didn't know about this one. Interesting. Got any more info for a girl?
I am so behind......Frito
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Post by fritolay66 on Nov 22, 2009 0:12:52 GMT -5
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Post by fritolay66 on Nov 22, 2009 0:33:49 GMT -5
Now for those pictures of the fiber in which I watched disappear into this sac type thing. This is the second experiment. Also, look at the "sewing thread" fiber very carefully. You will notice in the first picture that the fiber itself does not have anything in it. I watched bubble like things INSIDE of the fiber, travel its length prior to disappearing into the "sac-like" thing. The sac-like thing or puffy area in which it disappeared is seen in the superior aspect of the fiber. The end is where part of it is already in the "sac'. Those bubble type things can be seen in the second picture. Kam, do you think this could be how the fiber moves? Kinda of an internal undulation? Remember, the movement of the fiber into this sac, moved the entire specimen. So I am thinking the process of the penetration into the "sac" was a rather forceful one?
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