Shy, I believe these may be the point mutations you were looking for ,it is actually in 1q41 -1q42 region . It is a toll like receptor 5 (TRL5). An innate immune receptor for bacterial flagellin. This is associated with an increased risk of infection It maps to (allele C 1174T) contains common codon polymorph ism that aborgates signaling in S.L.E. (lupus). 1q42 is the region which is mutated in Variant Cystic Fibrosis Patients who are known for an increased risk of agrobactrium infection's . Also of great interest is the fact that agrobactrium not only changes the D.N.A. of it's host (plant) but it causes the body of the host (plant) to put out carbon and nitrogen on which it lives. Seems to me if it can chemically alter it's host that way that would explain the high density fiber produced in morgellons as well as the Chrystal's and bugs attracted to nitrogen. If it is stealing our D.N.A. wouldn't that leave us open to the replication of fortunistic D.N.A.. Sort of us being like a tree of life.
Sky, I believe these may be the point mutations you were looking for ,it is actually in 1q41 -1q42 region . It is a toll like receptor 5 (TRL5). An innate immune receptor for bacterial flagellin. This is associated with an increased risk of infection It maps to (allele C 1174T) contains common codon polymorph ism that aborgates signaling in S.L.E. (lupus). 1q42 is the region which is mutated in Variant Cystic Fibrosis Patients who are known for an increased risk of agrobactrium infection's . Also of great interest is the fact that agrobactrium not only changes the D.N.A. of it's host (plant) but it causes the body of the host (plant) to put out carbon and nitrogen on which it lives. Seems to me if it can chemically alter it's host that way that would explain the high density fiber produced in morgellons as well as the Chrystal's and bugs attracted to nitrogen. If it is stealing our D.N.A. wouldn't that leave us open to the replication of fortunistic D.N.A.. Sort of us being like a tree of life.
The only Neo I can think of that would be related to genetics would be the possible interest of neo-Nazi's in developing the supreme race and effectively eliminating the races of color. Is this the Neo you are referring to? I've seen the 1q42 mutations are prolific in the black population. If this is the case I believe thier little genetic mass experiment is going to backfire and possible produce a green hued race. As we become intermingled with plant D.N.A. Maybe the little green men are our offspring coming back thru time to pay a visit.
Last Edit: Aug 5, 2007 19:40:56 GMT -5 by lilsissy
You got me thinking about that neo thing Sky, I remember reading that the 1q42 mutation is prevalent in the black population. I have alot of American Indian bloodline and have also seen that mentioned alot in connection with 1q42. I have heard that Lupus which I have been diagnosed with is rare in whites. I always thought that I acquired this from my Indian Fore-fathers. Have you any reasons to believe this (morgellons) could be connected to Ethnic Cleansing?
Or what about this? DNA and RNA manipulations Total DNA from Leishmania was isolated using DNAzol (Gibco-BRL). Southern blotting, radiolabelling of DNA probes, hybridizations and washing conditions were performed following standard protocols (30). The NEO gene-specific probe was made by PCR. The GFP gene probe was generated by HindIII–XbaI digestion of vector phGFP-S65T. The trypanothione reductase (TR) gene (31) amplified by PCR was used as a control single-copy gene probe. The copy number of the different extrachromosomal vectors was estimated by comparative Southern blot hybridization. Quantitation of TR- and NEO-containing DNA fragments was done by densitometric analysis using a PhosphorImager with the ImageQuant3.1 software.
"pneuma primarily denotes "the wind" (akin to pneo, "to breathe, blow"); also "breath;" then, especially "the spirit," which, like the wind, is invisible, immaterial and powerful. The NT uses of the word may be analyzed approximately as follows: " www.antioch.com.sg/cgi-bin/bible/vines/get_defn.pl?num=2735
A European patent: ........"The present invention relates to viral vectors which are (a) self-replicating; (b) capable of systemic infection in a host; (c) contain, or are capable of containing, nucleic acid sequences foreign to the native virus, which are transcribed or expressed in the host; and (d) stable, especially for the transcription and expression of foreign nucleic acid sequences"
............"A given virus may contain either DNA or RNA, which may be either single- or double-stranded. The portion of nucleic acid in a virion varies from about 1% to about 50%. The amount of genetic information per virion varies from about 3 kb to 300 kb per strand. The diversity of virus-specific proteins varies accordingly. Examples of double-stranded DNA containing viruses include, but are not limited to, Hepatitis 8 virus, papovaviruses such as polyoma and papilloma, adenovirus, poxviruses such as vaccinia, caulimoviruses such as Cauliflower mosaic virus (CaMV), Pseudomonas phage PMS2, Herpesvirus, Bacillus subtilin phage SP8, and the T bacteriophages."
.........."Hosts which are capable of being transformed by these techniques include bacteria, yeast, fungus, animal cells and plant cells or tissue. Transformation is accomplished by using a vector which is selfreplicating and which is compatible with the desired host. The vectors are generally based on either a plasmid or a virus. Foreign DNA is inserted into the vector, which is then used to transform the appropriate host. The transformed host is then identified by selection or screening. For further information concerning the transformation of these hosts, see Maniatis, T. et al., Molecular Cloning (lust Ed.) and Sambrook, J. et al. (2nd Ed.), Cold Spring Harbor Laboratory, Cold Spring Harbor (1982, 1989); Molecular Cloninq, D.M. Clover, Ed., IRL Press, Oxford (1985); Grierson, D. et al. Plant MolecularBiology, Blackie, London, pp. 126-146 (1984), and Methods inEnzymologv, Vols. 68, 100, 101, 118 and 152-155 (1979, 1983, 1986 and 1987)." ...............
Representative viruses which are single-stranded DNA are the parvoviruses and the bacteriophagesFox174, fl and M13. Reoviruses, cytoplasmic polyhedrosis virus of silkworm, rice dwarf virus and wound tumor virus are examples of double-stranded RNA viruses. Singlestranded RNA viruses include tobacco mosaic virus (TMV), turnip yellow mosaic virus (TYMV) picornaviruses, myxoviruses, paramyxoviruses and rhabdoviruses. The RNA in single-stranded RNA viruses may be either a plus or a minus strand. For general information concerning viruses see Grierson, D. et al., Plant Molecular Biology, Blackie, London, pp. 126-146 (1984); Gluzman, Y. et al., Communications in Molecular Biology: Viral Vectors, Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988)."
Double standed and single stranded viruses,
;'Prior investigators have reported a maximum accumulation of a foreign protein in any genetically engineered plant of 2% of the total soluble protein.
Although the expression of potentially valuable proteins such as antibodies and human serum albumin has been reported previously (Laemmli, U. K. Nature 227:680-685 (1970); Bradford, M. M. Anal. Biochem.
72:248-254 (1976)) these were produced in Agrobacterium-mediated transgenic plants. A major difference between this plant viral expression system and previous methods is the quantity of protein produced and the amount of time required to obtain genetically engineered plants. Systemic infection and expression of a-trichosanthin occurred in less than two weeks while it takes several months to create a single transgenic plant."
more if here, lot of digging though.
I thought the Eureopeans where not using GM foods. or is it sneaked in.
Title: Universal stem cells United States Patent: 6,986,887 Issued: January 17, 2006 Inventors: Lawman; Patricia (Chipley, FL); Lawman; Michael J. P. (Chipley, FL) Assignee: Morphogenesis, Inc. (Oldsmar, FL) Appl. No.: 186231 Filed: June 28, 2002
The subject invention pertains to materials and methods for preparing multi-potential stem cells having a pre-selected expression of MHC antigens. Stem cells of the subject invention can be used to generate histocompatible tissues/organs for transplantation. The process of the subject invention comprises the use of targeting vectors capable of gene knockout, insertion of site-specific recombination cassettes, and the replacement of histocompatibility alleles in the stem cell. Novel knockout vectors are used to delete designated regions of one chromosome. Recombination cassette vectors are then used to delete the same region on the second chromosome and deposit a site-specific recombination cassette which can be utilized by replacement vectors for inserting the new MHC genes on the chromosome of the engineered cell. The subject invention also pertains to cells, tissues, and transgenic mammal prepared using the methods and materials of the invention.
"Yeast FLP-FRT Site-Specific Recombination System. The pHRC-DR recombination cassette can contain two FRT site-specific recombination sequences flanking bsd (see FIG. 3). The FRT consists of two inverted 13 bp repeats and an 8 bp spacer (O'Gorman, et al., 1991). Two FRTs from pNEOâGAL plasmid (Stratagene) can be used to flank the bsd gene. Bsd, under the control of the TK promoter can be readily obtained from pMC1bsd.A by digestion with Xho I and Sac II or from pGFP/TKpBSD with Bam HI and Bgl II. Alternatively, heterospecific lox sites could be utilized (Sauer 1996). These variant lox sites, having an altered spacer region are not proficient for Cre-mediated recombination with the canonical 34 bp loxP site, but can recombine with each other. By placing different heterospecific lox sites on different alleles, Cre can catalyze independent DNA recombination events at multiple loci in the same cells. "
.................... "In one embodiment of the methods of the invention, the selectable marker is a positive selectable marker gene, a negative selectable marker gene or both. Positive selection marker genes that can be used with the methods and vectors of the subject invention include green fluorescent protein (gfb), â-galactosidase (â-gal), blasticidin deaminase (bsd), dihydrofolate reductase (dhfr) and neomycin (neo). Negative selection marker genes that can be used with the methods and vectors of the subject invention include diptheria toxin-A (DT-A) and thymidine kinase (TK). Other suitable positive and negative selection marker genes are known in the art and can be used according to the present invention. "
"Claim 1 of 12 Claims
1. An in vitro method of altering the histocompatibility phenotype of a human stem cell, comprising:
a) deleting adjacent HLA-B and HLA-C MHC genes from a first chromosome;
b) replacing HLA-B and HLA-C MHC genes on a second chromosome with a site specific cassette, said cassette comprised within a vector that includes a site specific recombination sequence selected from LoxP and FRT;
c) inserting a transgene harboring a heterologous HLA allele and a homologous sequence matching the site specific recombination sequence of step b); and
d) catalyzing said site specific recombination wherein said transgene becomes incorporated into said second chromosome by site specific recombination to provide a stem cell with altered histocompatibility phenotype. "
pNEOâGAL plasmid this is the plasmid, binder (Stratagene) this is the company bacteriophage Cre-lox yeast FLP-FRT systems Recombination, Genetic Animals DNA, Fungal [chemistry] [metabolism] Eukaryotic Cells [physiology] Gene Targeting Humans Integrases [metabolism] Plasmids Schizosaccharomyces [genetics] [growth & development] Transposon Resolvases [metabolism] 0 (DNA, Fungal) EC 2.7.7.- (Cre recombinase) EC 2.7.7.- (Integrases) EC 2.7.7.- (Transposon Resolvases)
TYPE THEM IN SEE WHAT YOU FIND!!!!!!!!************@@@@@@@@+++++++++______
Site-specific recombination systems for the genetic manipulation of eukaryotic genomes. Information from Industry Pentasa® (mesalamine) A convenient UC agent with a top-to-bottom delivery system. Important Safety Information.
Full Prescribing Information.
Sign Up To Receive Medscape Best Evidence Key journal articles ranked for newsworthiness and clinical relevance in each specialty, linked to Medline abstracts. Genesis. 2006; 44(10):465-76 (ISSN: 1526-954X) Thomson JG; Ow DW Plant Gene Expression Center, USDA, Albany, California 94710, USA.
Site-specific recombination systems, such as the bacteriophage Cre-lox and yeast FLP-FRT systems, have become valuable tools for the rearrangement of DNA in higher eukaryotes. As a first step to expanding the repertoire of recombination tools, we screened recombination systems derived from the resolvase/invertase family for site-specific recombinase activity in the fission yeast Schizosaccharomyces pombe. Here, we report that seven recombination systems, four from the small serine resolvase subfamily (CinH, ParA, Tn1721, and Tn5053) and three from the large serine resolvase subfamily (Bxb1, TP901-1, and U153), can catalyze site-specific deletion in S. pombe. Those from the large serine resolvase subfamily were also capable of site-specific integration and inversion. In all cases, the recombination events were precise. Functional operation of these recombination systems in the fission yeast holds promise that they may be further developed as recombination tools for the site-specific rearrangement of plant and animal genomes.
Interpretive Summary: This article describes the demonstration of function of seven prokaryotic site-specific recombination systems in the fission yeast. Four of these are dedicated deletion systems, while the remaining three are integration systems that can also perform inversion and deletions. These new recombination systems represent additional genetic tools for the precise manipulation of eukaryotic genomes. Technical Abstract: Site-specific recombination systems, such as the bacteriophage Cre-lox and yeast FLP-FRT systems, have become valuable tools for the rearrangement of DNA in higher eukaryotes. As a first step to expanding the repertoire of recombination tools, we screened recombination systems derived from the resolvase/invertase family for site-specific recombinase activity in the fission yeast Schizosaccharomyces pombe. Here, we report that seven recombination systems, four from the small serine resolvase subfamily (CinH, ParA, Tn1721 and Tn5053) and three from the large serine resolvase subfamily (Bxb1, TP901-1 and U153) can catalyze site-specific deletion in S. pombe. Those from the large serine resolvase subfamily were also capable of site-specific integration and inversion. In all cases, the recombination events were precise. Functional operation of these recombination systems in the fission yeast holds promise that they may be further developed as recombination tools for the site-specific rearrangement of plant and animal genomes. "
WHEN THE PROKAYOTE INVADES THE EUKARYOTIC SYSTEM, EVOLUTION IS TAKING PLACE!
CAN YOU UNDERSTAND YET? PLEASE PEOPLE TYPE IN THE GENE NAMES ALL OF THEM......START READING THE PATENTS................THESE TOOLS ...........ARE BEING PATENTED TO CARRY ON THE OPERATION OF TRANSFORMATION.
PROKARYOTES ARE ONE CELLED, WE ARE MULTICELLED, BUT ONE=CELLED ARE BEING INCORPORATED INTO US TO MAKE PROKARYOTES SYMBIOTIC WITH THE HUMAN, ANIMALS, PLANTS.........
GAIA...........ONE EARTH, ONE BODY, ONE INTERACTING BEING...........
PROKARYOTES IN EUKARYOTES MAKES EVOLUTION HAPPEN, IT IS NOT EVOLUTION...........
PEOPLE ....................IT IS RECONSTRUCTION, DEVOLVING BACK TO WHERE THE LINK, THAT EVOLUTIONISTS THINK IS THERE, THE PROKARYOTES EVOLVED INTO THE EUKAROTES.
BUT,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,THESE ARE INVENTIONS,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,!!!!!!!!!!!!!
iF YOU CANNOT FIND EVOLUTION PROOF IN FOSSILS, IN THE SEASHELLS ON THE MOUNTAINTOPS, AND THOSE WHO KNOW THIS KNOW EXACTLY WHAT I MEAN,
YOU MUST REALIZE EVOLUTION IS STILLLLLLLLLLLL A THEORY...........YOU CANNOT PROVE IT, ONLY BY RECREATING WHAT YOU THINK IT IS, OR BY RE ESTABLISHING EVENTS THAT MAKE IT APPEAR IT HAPPENED.............YOU CONSTRUCT EVOLUTION...............IT IS CALLED EVOLUTIONARY DEVELOPMENT................... YOU MAKE IT HAPPEN.........................YOU INFER AND THIS IS WHAT INFERANCE DOES TO US. IT LIES TO US, IT IS NOT PROOF!
PROKAYOTES ARE USED IN THE CONSTRUCTION OF THESE INVENTIONS, CREATURES ARE BEING INVENTED,,,,,,,,,WE ARE BEING INVENTED.,,,,,,,,,,,,,,,
KEEP LOOKING FOR THE TOTAL HUMAN INVENTION IN FACT, I SHALL TYPE IT IN......
ONE WORD neo NEO PNEO pNEO the plasmid used with Agrobacterium.
This may not make sense but give me time............
We are not specimens..............we are not to be ethnically, medically experimented on when the experiments of the past created the diseases we have, and not fixing them has to take us back to prokaryotism.............TO RECREATE ALL OF LIFE?
THERE IS AND FOR A LONG TIME NOW BEEN CURES FOR EVERYTHING, BECAUSE ALL DISEASES ARE CAUSED BY GENETIC MANIPULATION, WHETHER IT BE PLANTS, FOR THE PROTEINS WE USE, ANIMALS FOR THE PROTEINS WE USE OR USE FOR THE PROTEINS THAT KEEP US ALIVE.
WHEN NEW PROTEINS ARE INFILTRATED INTO US BY WAY OF YEASTS, BY WAY OF ANIMALS WE EAT, BY WAY FOR VEGGIES WE EAT, FRUIT WE PARTAKE, THE WHOLE EVOLUTIONARY TREE OF LIFE HAS OVERTAKEN THE REAL TREE OF LIFE BECAUSE TRUTH JUST CANNOT BE ACCEPTED, TRUTH IS NEVER EASY, IT IS ALWAYS HARD, BUT THE WIDE AND EASY IS THE FASTEST WAY TOWARD THE SINGULARITY ISN'T IT?
THE BIG PICTURE IS BIG AND IT IS NASTY!!!!!!!!!!!!!!!!!!!!!!!!!!!@@@@@@@@@@@
HUMANS are still the same as when we first started, seems Evolutionists are stuck in the mud.
Last Edit: Aug 6, 2007 23:19:56 GMT -5 by skytroll
I don't know. Seems many scientists are -playing on both sides of the street.
Take for instance Nicholson.......given a reprieve......
but, Cold Spring Harbor is up to much........and they can do no wrong?
ARS..........plays the game........
There is a plan, and like I said long time ago, every agency, and Un congomerate is playing by their rules. It is sick, it is wrong, it is deceitful and it is most of all criminal.
It has been going on a long time........but, now they are really getting to the point a spanking is in order. or a slap side their intellectual heads, knock a few of those amyloids around.
Let them feel them and absorb the traveling fibrils.
Beginning with the S. Pombe.
And it all began with a yeast..........
Oh, and they are looking for :
Method for the prevention of fusarium diseases and microorganisms used for the same
"Document Type and Number:United States Patent 4988586 Link to this page:http://www.freepatentsonline.com/4988586.html Abstract:A method for the prevention of Fusarium diseases comprising the application of a microorganism that decomposes or detoxifies fusaric acid to the plant or to the soil. The microorganisms are the genuses Cladosporium and Pseudomonas that have the ability to decompose and/or detoxify fusaric acid. "
BRIEF DESCRIPTION OF THE DRAWING
This invention may be better understood and its numerous objects and advantages will become apparent to those skilled in the art by reference to the accompanying drawing as follows:
FIG. 1 is a restriction map of the gene obtained by this invention, which includes the plasmid pFS4.
................... [glow=red,2,300]And guess what this is![/glow]
Abnormality in catalase import into peroxisomes leads to severe neurologicaldisorder
PSF 1, 2, and 3 are chimeras.
We have studied human skin fibroblast cell lines that have multiple peroxisomal dysfunctions with normal packaging of PTS1 and PTS2 signal-containing proteins but lack catalase in peroxisomes. To understand the defect in targeting of catalase to peroxisomes and the loss of multiple enzyme activities, we transfected the mutant cells with normal catalase modified to contain either PTS1 or PTS2 signal sequence. We demonstrate the integrity of these pathways by targeting catalase into peroxisomes via PTS1 or PTS2 pathways. Furthermore, restoration of peroxisomal functions by targeting catalase-SKL protein (a catalase fused to the PTS1 sequence) to peroxisomes indicates that loss of multiple functions may be due to their inactivation by H2O2 or other oxygen species in these catalase-negative peroxisomes. In addition to enzyme activities, targeting of catalase-SKL chimera to peroxisomes also corrected the in situ levels of fatty acids and plasmalogens in these mutant cell lines. In normal fibroblasts treated with aminotriazole to inhibit catalase, we found that peroxisomal functions were inhibited to the level found in mutant cells, an observation that supports the conclusion that multiple peroxisomal enzyme defects in these patients are caused by H2O2 toxicity in catalase-negative peroxisomes. Moreover, targeting of catalase to peroxisomes via PTS1 and PTS2 pathways in these mutant cell lines suggests that there is another pathway for catalase import into peroxisomes and that an abnormality in this pathway manifests as a peroxisomal disease.
Keywords: peroxisomal disease, peroxisomal targeting signal
[glow=red,2,300]A peroxisomal targeting signal (PTS) is a region of the peroxisomal protein that receptors recognize and bind to.[/glow]
and the CDC is supposed to fix what is created? Tough job]. But, they get the raw deal, the end reactions, the call to put health in govt. hands.
I'm trying hard to understand all this . A lot of new stuff to me. I feel sometimes like I'm dancing as fast as I can round the edge's of Pandora's box. The more I discover the more there is to find. One thing I know for sure mankind sure is creative. A literal explosion of creativity seems to have occurred ........... The New Big Bang . Just in viewing all of the D.N.A. changes ....... I'm about to explode ..... but ........ I forgot for a minute ...... I have morgellons and I already got banged somewhere ......poop they took all the fun out of it too. Now that's enough to make you really wanna kick butt!!!!!!!! All you kingdom jumping scientists better watch out when word gets out your swapping D.N.A. with everybody's wife and husbands woooooowho
I was listening when he was even being poetic, and so much of what he said rings true.
I have not read his latest, but, am going down a few avenues that just might help.
Symbology is important to these folks, I had to learn how they thought first, before, I could go into the connections.
Science should be discussed and since it isn't we know an agenda is working top down, this is not bottom up, but the construction of their novel organisms is bottom up. So now we have double talk, rather than reverse psychology, two tools used to buffalo the people.
Only if Barry Goldwater and JFK could have talked directly to the people. If Tesla could have talked to the people, if Steno could have, if Bechamp could have. So much talent buried for an agenda.
But, as long as we have free speech, DO IT!
They have a specific tree they are putting all the new novelly created species in, we will have double species for a while. wonder if we are one of the humans, and the rest who don't get Morgellons are the original, we are the transformed ones. Whooooooooooohaaaaaaaaaaaaa
I know I hope Bill Gates don't screw up that Wormwood + agrobacterium D.N.A. malaria cure he's working on. Revelations speaks of a star falling from Heaven named WORMWOOD makes a third part of the rivers bitter . Many men die. He sure wouldn't be a star anymore and our increased knowledge we enjoy thru the vast expanses of the INTERNET would no longer be so heavenly. When man goes to and fro and knowledge is increased.
VIII.A. Cytochemical Stains Cytochemical stains can help in the identification of and discrimination between
Organisms (e.g. bacteria, fungi) Enzymes (e.g. chemical substances, such as acid phosphatase) Other intra and extracellular substances (e.g. fat, mucin, amyloid, collagen, melanin) The appropriate use of controls is of utmost importance in cytochemical stains. Refer to VIII.G for further discussion .................. VIII.C. Transmission Electron Microscopy Although used extensively in the 1970’s and 1980’s, immunocytochemical stains have supplanted transmission electron microscopy as an ancillary test, except in certain circumstances. Cytologic preparations are ideally suited for this study because of the large number of cells they contain and because they are often a single cell type. Certain neoplasms have ultrastructural qualities that may help in their identification. The high magnification of electron microscopy elucidates specific ultrastructures85 such as,
Extracellular features (e.g. amyloid) Cell surface features (e.g. microvilli, intercellular junctions) Cytoplasmic features (e.g. mitochondria, lysosomes, neurosecretory granules, melanosomes) Nuclear features (e.g. inclusions) Other (e.g. viral particles) ........................................
THE EVOLVING APPLICATION OF THE WRITTEN DESCRIPTION REQUIREMENT TO
"A United States patent must provide a "written description" of the invention claimed therein. In its earliest implementation, a patent's written description fulfilled a notice function of putting the public in possession of the boundaries of the patentee's property right. Under modern practice, that notice function is instead performed by the claims of the patent. In 1967, the Court of Customs and Patent Appeals (CCPA) breathed new life into the written description requirement of section 112 of the Patent Act, by applying it for a different purpose, that of ensuring "support" for claims first presented or substantively amended after a patent application has been filed. The court viewed written description compliance as a means of ensuring that the patent applicant had actually invented the later-claimed subject matter as of the earlier filing date of the application. The CCPA and its successor, the Court of Appeals for the Federal Circuit, repeatedly recognized that the manner in which the written description was provided was not critical, so long as those of ordinary skill would understand the newly-claimed subject matter to be fairly within the patentee's original contribution.
Written description jurisprudence significantly diverged from these principles with the Federal Circuit's 1997 decision in Regents of the University of California v. Eli Lilly. In Lilly, the court applied the written description requirement to claims originally filed in a pioneering University of California patent application directed to the recombinant production of insulin, and held that the written description requirement is not satisfied for claims to DNA absent an express disclosure of nucleotide sequence. The Lilly decision may profoundly limit the scope of protection available for new gene inventions; viewed in tandem with recent decisions interpreting the enablement requirement of § 112 of the Patent Act, it represents the latest advance in an ominous trend towards imposition of uniquely heightened patentability requirements for biotechnological inventions. Lilly aptly illustrates the increased widening of the gulf between the norms of the business and scientific communities and the U.S. patent system, as users of the latter come to understand that the patent system no longer reflects the realities of scientific contribution."
"Users of the patent system are justified in viewing the Lilly decision as reflecting an increasingly-widening gulf between the norms of the business and scientific community and those of the United States patent system. Persons skilled in the art of recombinant DNA technology were very likely to have understood that by making the rat insulin cDNA, the UC inventors conceptually possessed the human insulin cDNA (if not all mammalian cDNAs). But under the Lilly court's heightened "physical possession" standard for written description compliance, UC was denied any significant reward for its breakthrough contribution. Rather than awarding patent protection to the first to make it possible to clone a particular gene family, the written description standard of Lilly requires that the patent right go to the first firm to sequence a number of the genes (or, perhaps, even the first to correctly guess their sequence). The firm with the fastest or most accurate cloning and sequencing team will reap the benefits of an invention made possible by the pioneering research of others. The credibility of the patent system suffers as users come to recognize that it no longer reflects the realities of scientific contribution. " www.law.berkeley.edu/journals/btlj/articles/vol13/Mueller/html/text.html
THAT WOULD those ........"skilled in the art of recombinant DNA technology".........
So, the patent system is the way in for all this crap, and you have to know the art?
Who left and appointed the patent department the KING? or would that be the Pharma companies or would that be the University seeing no wrong in patenting human insulin. Come to find out later, that I believe that insulin that was made actually caused some deaths.
recombinant DNA technology: "Recombinant DNA From Wikipedia, the free encyclopedia (Redirected from Recombinant DNA technology) Jump to: navigation, search
GloFish are a type of zebrafish with recombinant DNA. Genes for fluorescent proteins have been inserted into their genome to produce their fluorescent colors.Recombinant DNA is a form of artificial DNA which is engineered through the combination or insertion of one or more DNA strands, thereby combining DNA sequences which would not normally occur together. In terms of genetic modification, recombinant DNA is produced through the addition of relevant DNA into an existing organismal genome, such as the plasmid of bacteria, to code for or alter different traits for a specific purpose, such as immunity. It differs from genetic recombination, in that it does not occur through processes within the cell or ribosome, but is exclusively engineered.
The Recombinant DNA technique was engineered by Stanley Norman Cohen and Herbert Boyer in 1973. They published their findings in a 1974 paper entitled "Construction of Biologically Functional Bacterial Plasmids in vitro", which described a technique to isolate and amplify genes or DNA segments and insert them into another cell with precision, creating a transgenic bacterium. Recombinant DNA technology was made possible by the discovery of restriction endonucleases by Werner Arber, Daniel Nathans, and Hamilton Smith, for which they received the 1978 Nobel Prize in Medicine"
An example of chimeric plasmid formation from two "blunt ends" via the enzyme, T4 Ligase.Main article: Chimeric DNA When recombinant DNA is then further altered or changed to host additional strands of DNA, the molecule formed is referred to as "chimeric" DNA molecule, with reference to the mythological chimera which consisted as a composite of several animals. The presence of chimeric plasmid molecules is somewhat regular in occurrence as throughout the lifetime of an organism the propagation by vectors ensures the presence of hundreds of thousands of organismal and bacterial cells which all contain copies of the original chimeric DNA.
In the production of chimeric plasmids, the processes involved can be somewhat uncertain as the intended outcome of the addition of foreign DNA may not always be achieved and may result in the formation of unusable plasmids. Initially, the plasmid structure is linearised to allow the addition by bonding of complimentary foreign DNA strands to single-stranded "overhangs" or "sticky ends" present at the ends of the DNA molecule from staggered, or "S shaped" cleavages produced by restriction endonucleases.
I don't want to keep pestering y'all, but as soon as sky posted these late, late at night, suddenly there were 22 unregistered people on board checkin' it all out. PLEASE READ THESE.
I know it is hard to understand. It all ties in to the agro, bugs, fungus. etc. Please consider and inform yourselves. I do not want to believe this either. I don't. But this is actually happening to us. Remember UKguys grass thingy? Today I WATCHED as one came out of the skin on my abdomen. Almost identical. PLANT, INSECT,YEAST, FUNGI, HIGHER PRIMATE. RECOMBINATION.
Carrie, you said it is flukes and you don't believe in any of the fungi or gm stuff. HOW then, do you justify the results of Citovsky? Do you just blow them off 'cause they don't fit? Not picking on you as I love your spirit and your determination and you are a mom of lil ones like me, but I really want to know? Carrie, I need to call ya, cuz I originally thought it was flukes, but now. . plant material is extruding from my skin along with hair. and connect the friggin dots on my skin. PM me if you read this.
THE TRUTH IS THE GREATEST ENEMY OF THE STATE." -- Joseph Goebbels, German Minister of Propaganda, 1933-1945
ChasSansc2: This story is a little different than the original one I read, where the writer described a test you can perform on your clothing. It consisted of wetting the clothing, placing it in a microwave until hot to the touch, then placing the clothing
May 18, 2019 21:49:28 GMT -5
ChasSansc2: (using tongs) into cold water with lemon juice in it. Wring them out afterwards above the cold water, and look for very small "tiny black rice" looking things at the bottom.
May 18, 2019 21:50:48 GMT -5
ChasSansc2: I did, and I saw, and I was in shock. From that point on I added 1 cup of LEMON JUICE to every load of laundry I did. After doing this, I noticed that my clothes actually felt lighter before putting them in the dryer.
May 18, 2019 21:52:07 GMT -5
ChasSansc2: What does this all mean? I would suggest that anyone who has Morgellon to add 1 cup of LEMON JUICE to every load of laundry they do, start drinking lemon water and maybe even start taking baths with at least 2 cups of LEMON JUICE in it.
May 18, 2019 21:54:45 GMT -5
ChasSansc2: I used straight LEMON JUICE on my body, as well as Bragg's Apple Cider Vinegar. A word of caution, LEMON JUICE on the face can feel like Mace (it burns), so, you may want to dilute it before applying the LEMON JUICE soaked wash cloth.
May 18, 2019 21:57:23 GMT -5
ChasSansc2: Great - this won't let me scroll back up - STUPID.
May 18, 2019 22:00:11 GMT -5
poi.k,hjmv: I applied to this forum 2017 and still not approved why not tell them they are not approved??? you must enjoy seeing peoplesufferandleftfordead RUDE
Jun 25, 2019 22:03:42 GMT -5
prson who agrees w poi.k,hjmv:: LOL @ poi.k,hjmv. . . so far same here. but hopefully not for TOO long
Aug 12, 2019 3:35:04 GMT -5
thinkwithwomen: what are women have health & fitness issues:)- we understood the importance of women health and fitness.we will discuss and share fitness stories and success stories we taken care of women for the age like premature and mature during pregnancy
Nov 22, 2019 6:11:16 GMT -5
DAC: I have it, but it's not real bad for me yet. I do not have any sores on my body, but the stinging and moving under my skin is very bad. I had a friend who died from this. He got it from cutting down a tree by the airport. He
Dec 25, 2019 5:25:05 GMT -5
Aitche: Check out images and videos of trichinosis think you will be
Jan 22, 2020 7:29:49 GMT -5