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Post by skytroll on Jul 21, 2008 13:45:51 GMT -5
This just in....... AND......this indicates that DNA mutagenesis is going on. So, not only from radiation, and I do not think is from pure Sun, put from radiation and the attempts to use bioremediation to clean it up in the environment and DNA manipulation recombinations in gm foods. Also our food is radiated? ================= Press Release attached as .pdf file, and text below: Taking Wellness to the World® ENIVA VIBE® NUTRACEUTICAL TESTED AT LINUS PAULING MICRONUTRIENT RESEARCH INSTITUTE AND FOUND TO POSSESS DNA PROTECTIVE PROPERTIES FOR IMMEDIATE RELEASE JULY 2008 Minneapolis, MN) The Eniva Research Group has released results from laboratory testing by the world-famous Linus Pauling Micronutrient Research Institute at Oregon State University which demonstrated the Eniva Health Supplement VIBE® possessed DNA protective anti-mutagenic activity for human cells. This third party testing was performed as part of an ongoing investigative effort to further identify mechanisms by which the Eniva VIBE nutraceutical impacts human health. Chief Scientific Officer for Eniva Corporation, Dr. Benjamin Baechler, stated, “While these results are tremendously exciting and give us insight into one of the mechanisms of action of the VIBE nutraceutical, we must be careful not to generalize these results beyond the study findings. What we can say for certain is these results further demonstrate the ability of the VIBE nutraceutical to help support healthy and normal functioning of human cells, even under very stressful physiologic situations.” The research design consisted of culturing human cells with and without the VIBE nutraceutical and then exposing these human cells to a well known DNA mutagen–ultraviolet radiation. After a period of time, the human skin cells were then obtained and examined for specific damage to their DNA. Results were compared between the human cells supported by VIBE in their growth medium and those without it. The results demonstrated an extremely statistically significant decrease in DNA damage in those human cells which were supported with the VIBE nutraceutical (p-value < 0.001). The exact laboratory technique used was the COMET assay, otherwise known as Single- Cell Gel Electrophoresis (SCGE), which is a very sensitive and well known peer accepted laboratory method for assessing damage to cellular DNA. The COMET assay is widely used to assess DNA damage in cancer research, environmental toxicology and radiation biology. After mutagen exposure, cells are embedded in agarose gel on a microscope slide, lysed, electrophoresed, and then stained with fluorescent DNA binding dye. [glow=red,2,300]Damaged DNA migrates during the process, forming a shape often described as a “comet.”[/glow] The specific pattern is then automatically quantified through laser assisted computer analysis. Through specific algorithms, DNA damage can then be quantified and trends evaluated. These results are part of an intensive initiative by the Eniva Corporation to further explore the mechanism of action behind their nutraceutical products. Chairman of Eniva Corporation, Andrew Baechler, commented, “This result further identifies and separates Eniva as a leader in providing science-based dietary solutions. We are very enthusiastic about the findings and are very grateful to our collaborators at the Linus Pauling Micronutrient Research Institute.” Eniva Corporation is a manufacturer and global marketer of high quality, science-based dietary supplements known as nutraceuticals. It carries a product line of over 75 wellness products, ranging from cardiovascular to general health and wellness. The corporation recently celebrated its tenth year anniversary at its world headquarters in Anoka, Minnesota. More information on this topic and the actual testing data can be found at www.enivanutraceutics.com. Research study references on file, Eniva USA, 2008. 9702 Ulysses Street NE, Minneapolis, MN 55434 USA • tel: 763-795-8870 • fax: 763-795-8890 • www.eniva.com 0611.07.01.08.REV.01 100% recycled paper with 30% post-consumer waste. Please recycle. Taking Wellness to the World® 9702 Ulysses Street NE, Minneapolis, MN 55434 USA • tel: 763-795-8870 • fax: 763-795-8890 • www.eniva.com 0611.07.01.08.REV.00 100% recycled paper with 30% post-consumer waste. Please recycle. More info later on what causes DNA damage.........
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Post by skytroll on Jul 21, 2008 13:53:52 GMT -5
Evidently, we need to repair on the cell level: Other Mutagenisis factors that may cause DNA mutation: ============================== DNA damage and mutation in human cells exposed to nitric oxide in vitro Abstract Nitric oxide (NO.) is a physiological messenger formed by several cell types. Reaction with O2 forms oxides that nitrosate amines at pH values near 7. We now report experiments in which NO. was added to intact human cells and to aerobic solutions of DNA, RNA, guanine, or adenine. TK6 human lymphoblastoid cells were mutated 15- to 18-fold above background levels at both the HPRT and TK gene loci. Xanthine and hypoxanthine, from deamination of guanine and adenine, respectively, were formed in all cases. NO. induced dose-responsive DNA strand breakage. Yields of xanthine ranged from nearly equal to about 80-fold higher than those of hypoxanthine. Yields of xanthine and hypoxanthine from nucleic acids were higher than those from free guanine and adenine. This was most pronounced for xanthine; 0.3 nmol/mg was formed from free guanine vs. 550 nmol/mg from calf thymus RNA. Nitric oxide added to TK6 cells produced a 40- to 50-fold increase in hypoxanthine and xanthine in cellular DNA. We believe that these results, plus the expected deaminations of cytosine to uracil and 5-methylcytosine to thymine, account for the mutagenicity of nitric oxide toward bacteria and mammalian cells. www.pnas.org/content/89/7/3030.abstractother mutatgenesis: HSV: ================ Activation of Ataxia Telangiectasia-mutated DNA Damage Checkpoint Signal Transduction Elicited by Herpes Simplex Virus Infection* ukaryotic cells are equipped with machinery to monitor and repair damaged DNA. Herpes simplex virus (HSV) DNA replication occurs at discrete sites in nuclei, the replication compartment, where viral replication proteins cluster and synthesize a large amount of viral DNA. In the present study, HSV infection was found to elicit a cellular DNA damage response, with activation of the ataxia-telangiectasia-mutated (ATM) signal transduction pathway, as observed by autophosphorylation of ATM and phosphorylation of multiple downstream targets including Nbs1, Chk2, and p53, while infection with a UV-inactivated virus or with a replication-defective virus did not. Activated ATM and the DNA damage sensor MRN complex composed of Mre11, Rad50, and Nbs1 were recruited and retained at sites of viral DNA replication, probably recognizing newly synthesized viral DNAs as abnormal DNA structures. These events were not observed in ATM-deficient cells, indicating ATM dependence. In Nbs1-deficient cells, HSV infection induced an ATM DNA damage response that was delayed, suggesting a functional MRN complex requirement for efficient ATM activation. However, ATM silencing had no effect on viral replication in 293T cells. Our data open up an interesting question of how the virus is able to complete its replication, although host cells activate ATM checkpoint signaling in response to the HSV infection. www.jbc.org/cgi/content/abstract/280/34/30336================== What Causes DNA Mutations? Mutations in DNA sequences generally occur through one of two processes: 1. DNA damage from environmental agents such as ultraviolet light (sunshine), nuclear radiation or certain chemicals 2. Mistakes that occur when a cell copies its DNA in preparation for cell division. ......more here learn.genetics.utah.edu/units/disorders/sloozeworm/mutationbg.cfmSkytroll
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Post by skytroll on Jul 21, 2008 14:03:22 GMT -5
PHOSPHORYLATION and the p53.....keeps coming up over and over Lil Sissy! www.jbc.org/cgi/content/abstract/278/17/14806MUTATIONS in ATM THIS IS BIG because this is where the ARtificial signals can disrupt. Ataxia telangiectasia mutated (ATM) is a serine/threonine-specific protein kinase (EC 2.7.11.1) that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Several of these targets, including p53, CHK2, BRCA1, and H2AX are tumor suppressors. The protein is named for the disorder Ataxia telangiectasia caused by mutations of ATM. The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. LIL SIS.............note hte autosomal recessive disorder connotation. WE...... have this, those who carry these AUTOSOMAL RECESSIVE DISORDER......this was not a disorder for carriers of diseases, now it is, because that second X was activiated at some point. We then become manifesting carries, meaning we start to manifest these certain auto recessive genes, THAT are supposed to remain recessive, do not affect us, but now with ATM and ATP signals have ifiltrated those recessive genes, we show signs of the diseases we carry but we are not supposed to get, however, it may be passed down to children. Hence the 1q. 42 along with other auto recessive diseases. This are found in the Mendal OMIM......... Done by vaccines, altered vaccinces, recombination of vaccines and gene transfer from viruses. However, what else is indicated are the SIGNALS. Elecrolytes?.......... This can be done directly into any DNA, RNA. Dr. H. mentioned this: siRNA,,,,,,, this is where the signal comes in? not sure the connection there, but there is one. Skytroll SKytroll
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Post by skytroll on Jul 21, 2008 14:14:51 GMT -5
Human cells do not normally support productive bovine adenovirus type 3 (BAdV-3) infection. Here, the outcome of BAdV-3 infection of both 293 cells and 293 cells modified to constitutively express the simian virus 40 (SV-40) T antigen (293T cells) was studied. Whereas BAdV-3 could efficiently infect 293 cells, there was a block in virus DNA replication, late-gene expression and virus production. In contrast, replication and efficient virus production could be detected in 293T cells infected with BAdV-3 or transfected with a replication-competent genomic BAdV-3 clone (pFBAV304). Early-phase gene expression was detected readily in both BAdV-3-infected 293 and 293T cells. However, the progression to efficient viral DNA synthesis and late-phase protein synthesis occurred only in 293T cells. Electron microscopy and virus growth kinetics demonstrated the formation of progeny virus in 293T cells. The SV-40 T antigens act to overcome a barrier in BAdV-3 DNA replication in 293 cells. vir.sgmjournals.org/cgi/reprint/87/4/817.pdfthe tainted SV40 rises again.......................... liver, kidneys, lungs....... all related to damage. So, DNA damage can occur other than in radiation......... Radiation, chemical reactions, recombinations, vaccines, bioremediation with hydrovent bacterias and viruses. siRNA is the key here. tinyurl.com/5h6lpxwww.google.com/search?hl=en&client=firefox-a&channel=s&rls=org.mozilla:en-US:official&hs=KHg&defl=en&q=define:siRNA&sa =X&oi=glossary_definition&ct=title skytroll p53 cells 293T cells
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Post by skytroll on Jul 21, 2008 14:25:30 GMT -5
Hela cells are another, which where contaminated cells that were spread from lab to lab these are the cancer cells. They were found in many labs around the world. Apoptosis, means cell death. We have previously shown that expression of the papillomavirus E2 protein in HeLa cells induces p53 accumulation and causes both cell cycle arrest and apoptosis. In contrast to growth arrest, onset of apoptosis was not correlated with an increase of p53 transcriptional activity. In the present study, we conducted biochemical and genetic experiments in order to determine whether E2-induced apoptosis was independent of p53 induction. We showed that E2 did not alter the transcription of Bax, a known p53-activated cell death inducer. The time course of apoptotic cell death preceded p53 induction by several hours. Overexpression of the HPV18 E6 oncogene prevented E2-mediated p53 accumulation, but did not alter the rate of cell death. Finally, point mutants of the HPV18 E2 transactivation domain induced apoptosis, although they were unable to induce high p53 accumulation or cell cycle arrest. In addition, the results obtained with these mutants indicated that both transcriptional activation and replication functions of E2 were dispensable for the induction of cell death. These observations show that E2-induced apoptosis is an early event, independent of p53 accumulation and unrelated to downstream p53-dependent transcriptional events. replicates..................... www.nature.com/onc/journal/v18/n32/abs/1202818a.htmlwhat is p53 accumulation? note, the COMET assay reference here: Arsenic compounds are potent human carcinogens. Accumulated evidence has shown that arsenite-induced cytogenetic alterations are associated with the carcinogenicity of arsenic. Because p53 plays a guarding role in maintaining genome integrity and accuracy of chromosome segregation, the mechanistic effects of arsenite on p53 activation were analyzed. In the present study, arsenite-induced DNA strand breaks were confirmed by alkaline single-cell gel electrophoresis (comet assay) in human fibroblast (HFW) cells. Accompanying the appearance of DNA strand breaks was a significant accumulation of p53 in arsenite-treated HFW cells, as demonstrated by immunoblotting and immunofluorescence techniques. p53 downstream proteins, such as p21 and the human homologue of murine double minute-2, were also significantly induced by arsenite treatment. Cell cycle retardation and G2-M arrest were observed in 5-bromo-2'-deoxyuridine pulse-labeled HFW cells by flow cytometry. Wortmannin, an inhibitor of phosphatidylinositol 3-kinases, inhibited arsenite- or X-ray irradiation-induced p53 accumulation but did not alter UV irradiation- or N-acetyl-Leu-Leu-norleucinal-induced p53 accumulation. p53 phosphorylation on serine 15 was also confirmed by immunoblotting technique in arsenite- and X-ray-treated HFW cells but was not observed in UV- or N-acetyl-Leu-Leu-norleucinal-treated HFW cells. These results suggest the involvement of a phosphatidylinositol 3-kinase-related protein kinase in arsenite-induced p53 accumulation. For confirmation, we demonstrated that arsenite treatment, similar to X-ray irradiation, did not induce p53 accumulation in GM3395 fibroblasts derived from a patient with ataxia telangiectasia. In contrast, UV irradiation did cause p53 accumulation in these cells. Together, these findings infer that arsenite-induced DNA strand breaks may lead to p53 phosphorylation and accumulation through an ataxia telangiectasia mutated-dependent pathway in HFW cells. www.ncbi.nlm.nih.gov/pubmed/11103796ARSENIC induces this! HFW cells is where they accumulate: HFW cells: check out these: tinyurl.com/5al3cnRelation to Arsenic?............... Skytroll
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Post by bannanny on Jul 21, 2008 14:27:30 GMT -5
I've only read your first post on this thread so far sky, but I just had to tell you this. I have the Vibe, Dr. Hildy gave it to me along with my other things to drink, including Via Viente. I mix them together, but I gotta say I forget to drink it every day. I'm gonna start tho, RIGHT NOW! Thanks so much for this info....... more hope on the way for my mindset!
love you ~~ bannanny
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Post by skytroll on Jul 21, 2008 14:39:38 GMT -5
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Post by zabrubon on Jul 21, 2008 19:23:23 GMT -5
Skytroll, we need laymen terms. This is just too much to read for us average brained persons. I want to read it but then I get lost in it all and frustrated. What is the gist of it please?
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Post by bessie on Jul 21, 2008 19:40:13 GMT -5
Sky - I posted the same thing this morning! No responses, though. As that is what agrobacteria does, I'm glad I use it. Bessie
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Post by bessie on Jul 22, 2008 14:17:43 GMT -5
Just came across this statement attributed to Citovsky:
"According to a statement issued by Professor Citovsky, this observation does not imply that Agrobacterium causes Morgellons or that Morgellons is indeed an infectious disease. However, it does encourage future studies to determine (1) statistical significance of data, (2) whether Agrobacterium is not only present extracellularly, but also causes genetic transformation of the infected tissues, and (3) whether infection of laboratory animals with Agrobacterium can recreate symptoms of Morgellons."
As agrobacterium operates through injecting its dna into the dna of the host, and we are hosts, then we should try to protect our dna. If Vibe works for that, we should use it.
Bessie
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Post by bannanny on Jul 22, 2008 14:41:59 GMT -5
Like I said, I'm using it and I'll let you know if I see any results! Not quite sure what to look for tho, just to feel better I guess.
The ingredients are as follows.......
Boron, germanium, strontium, sulphur, vanadium
Cranberry, raspberry, blueberry, blackberry, strawberry, cherry, carrot, acai berry, elderbury, hibiscus (flower), lemon, lime, apple, orange, blackcurrent, oregano, chokeberry, grape, pumpkin, tomato, pomegranate, wolfberry (gojiberry), stevia (leaf), grapeseed extract, citrus bio flavanoids.
D-Ribose, COQ10, L-carnitine, malic acid, isolated lecithin, mixed tocopherols.
Green tea leaf extract (water decaffeinated), L-Lysine, L-Proline, aloe vera gel, glucosamine HCI (vegetable), glycine, alanine, valine, isoleucene, leucene
love ~~ bannanny
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Post by skytroll on Jul 22, 2008 19:31:42 GMT -5
Chicago Bonnie, Our cells are marching in the wrong direction, this vibe will help them go right. In otherwords it will feed the cells on that level, and set them right, to be working as they should. Our cells have been hijacked....... because of errors in the medical world, and scientific world, of which no one will admit. What has happened is many of these releases, and mistakes were covered. Our cells have been damaged, ..... we are in survival mode, so to increase the army, we have to feed the cells, the right stuff, not the gm crap. If we do not understand how cells/DNA/RNa work we won't get the main point. That is why I put this here, will try to break it down. better. sorry. This is not easy, trying to find what is being hidden, covered up, because mistakes were made. period. And, we need to what genes, if any are involved, what genotypes, phenotypes, etc....... If cancer is from viruses, then we know that we are not given the right information. Genes do not cause cancer, carrying a virus is not like genetic makeup. It is VIRUSES that make the MUTATIONS. Okay look at MU................ It is a virus, it mutates............. These seem to know about MU phage: genesdev.cshlp.org/cgi/issue_pdf/advertising_pdf/10/13.pdfThe lie we have been told: GENES JUMP! I don't think so but this is what they are telling us. MU............means mutates, that is not the same as jumping...................How the lies proceed. Viruses and phages mutate bacteria genes inside us....this includes agrobacterium. The first machine was the viral bacteriophage................ www.biochem.uwo.ca/fac/chaconas/chaconas.htmlagain...........mutations are not Jumping genes.......... Viruses are involved with the cells. Skytroll
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Post by bessie on Jul 22, 2008 20:35:31 GMT -5
Or, as I said above, the agrobacteria that we all have (Morgies) is a bioengineered bacteria that is used to genetically modify plants. The scientists put whatever genetic material they want to inject into a plant into the agrobacteria. The agro then attacks the plant and INJECTS this new dna into the plant, altering it forever. That same bacteria is on us. If we do all we can to protect our dna, we might remain "human", rather than whatever hybrid this bug is turning us into. Bessie
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Post by zabrubon on Jul 22, 2008 20:46:37 GMT -5
Skytroll that helps. Thanks. I understand DNA and RNA but I just can't grasp some of this stuff quick enough and I appreciate you breaking it down.
I have given up on regular foods and eat only the organics now. Expensive but don't care, I would rather eat organic peanut butter sandwiches than GM Lobster tail.
ChicagoBonnie
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Post by scrambo on Jul 22, 2008 22:17:42 GMT -5
skytool, This appears to be a multi-level marketing product. Are you selling it?
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Post by glennb on Jul 23, 2008 0:54:47 GMT -5
Interesting info. Thanks skytroll. what about electro magnetic radiation from microwave, cell phone, Haarp etc
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Post by skytroll on Jul 23, 2008 0:59:11 GMT -5
Scrambo,
No, I don't sell it, but Dr. Hildegarde Staninger recommends it.
skytroll
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ara
New Member
Posts: 16
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Post by ara on Jul 23, 2008 2:54:22 GMT -5
Lead hydrogen arsenate was used well into the 20th century as an insecticide on fruit trees.
Its use sometimes resulted in brain damage to those working the sprayers.
In the last half century, monosodium methyl arsenate (MSMA), a less toxic organic form of arsenic, has replaced lead arsenate's role in agriculture.
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Post by liatris on Jul 23, 2008 6:41:44 GMT -5
Like I said, I'm using it and I'll let you know if I see any results! Not quite sure what to look for tho, just to feel better I guess. The ingredients are as follows....... Boron, germanium, strontium, sulphur, vanadium Cranberry, raspberry, blueberry, blackberry, strawberry, cherry, carrot, acai berry, elderbury, hibiscus (flower), lemon, lime, apple, orange, blackcurrent, oregano, chokeberry, grape, pumpkin, tomato, pomegranate, wolfberry (gojiberry), stevia (leaf), grapeseed extract, citrus bio flavanoids. D-Ribose, COQ10, L-carnitine, malic acid, isolated lecithin, mixed tocopherols. Green tea leaf extract (water decaffeinated), L-Lysine, L-Proline, aloe vera gel, glucosamine HCI (vegetable), glycine, alanine, valine, isoleucene, leucene love ~~ bannanny Regarding all those dehydrated berry bits - you could really benefit a lot more by eating a small bowl of the actual berries. Unless they are having you consume huge amounts of the product, the actual berries would give you more volume than what goes onto a few spoonfuls. Also, generally, fruits are better when they are not dehydrated - a lot of the nutrients are destroyed.
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jlk
Full Member
Posts: 146
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Post by jlk on Jul 23, 2008 8:45:41 GMT -5
This all goes right back in the direction of Dr. W. John Martin's theory who has been telling us all along "this is all developing from a mutation of a virus". Our cells are damaged by the virus and then it all manifests differently in varioius people. I had not made the insect connection but now I think that agricultural scientists wanted to create an insect that would help plants defend themselves against other insects and this GM food has back-fired and we now have bugs growing in human tissues..... or something like this.
One of the best power juices to help with this disease is Mangosteen - it has to have all the fruit parts in it - the best prices found were from vita.com not that the others would not be a good dietary addition but I have done most of them and I have found high quality Mangosteen to be as good as any. Sometimes really good stuff comes from MLM companies (and sometimes not) but the whole process is just a pain!
Thank you Skytoll for all your incredible research ability and time spent on this. You are something else gurl! Judy
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