Post by gradysghostii on Aug 21, 2008 18:39:42 GMT -5
Toni, excellent, I had chronic sinus infections before all this started and a sore throat to go with it, it's gone now, I think getting my PH in order cleared that up along with ACV but still, there is something to this.
I wonder if this could be tied into the attempts to clean up the MTBE's, I know it's used in oil spill cleanup and I think when they remove underground gas tanks from filling stations, putida (especially this hopped up kind) is probably everywhere in the ground water since it's contaminated with pretroleum products, just a thought.
Look at this:
Activity and three-dimensional distribution of toluene-degrading Pseudomonas putida in a multispecies biofilm assessed by quantitative in situ hybridization and scanning confocal laser microscopy
S Moller, AR Pedersen, LK Poulsen, E Arvin and S Molin
Department of Microbiology, Technical University of Denmark, Lyngby, Denmark.
As a representative member of the toluene-degrading population in a biofilter for waste gas treatment, Pseudomonas putida was investigated with a 16S rRNA targeting probe. The three-dimensional distribution of P. putida was visualized in the biofilm matrix by scanning confocal laser microscopy, demonstrating that P. putida was present throughout the biofilm. Acridine orange staining revealed a very heterogeneous structure of the fully hydrated biofilm, with cell-free channels extending from the surface into the biofilm. This indicated that toluene may penetrate to deeper layers of the biofilm, and consequently P. putida may be actively degrading toluene in all regions of the biofilm. Furthermore, measurements of growth rate-related parameters for P. putida showed reduced rRNA content and cell size (relative to that in a batch culture), indicating that the P. putida population was not degrading toluene at a maximal rate in the biofilm environment. Assuming that the rRNA content reflected the cellular activity, a lower toluene degradation rate for P. putida present in the biofilm could be estimated. This calculation indicated that P. putida was responsible for a significant part (65%) of the toluene degraded by the entire community.
I wonder if this could be tied into the attempts to clean up the MTBE's, I know it's used in oil spill cleanup and I think when they remove underground gas tanks from filling stations, putida (especially this hopped up kind) is probably everywhere in the ground water since it's contaminated with pretroleum products, just a thought.
Look at this:
Activity and three-dimensional distribution of toluene-degrading Pseudomonas putida in a multispecies biofilm assessed by quantitative in situ hybridization and scanning confocal laser microscopy
S Moller, AR Pedersen, LK Poulsen, E Arvin and S Molin
Department of Microbiology, Technical University of Denmark, Lyngby, Denmark.
As a representative member of the toluene-degrading population in a biofilter for waste gas treatment, Pseudomonas putida was investigated with a 16S rRNA targeting probe. The three-dimensional distribution of P. putida was visualized in the biofilm matrix by scanning confocal laser microscopy, demonstrating that P. putida was present throughout the biofilm. Acridine orange staining revealed a very heterogeneous structure of the fully hydrated biofilm, with cell-free channels extending from the surface into the biofilm. This indicated that toluene may penetrate to deeper layers of the biofilm, and consequently P. putida may be actively degrading toluene in all regions of the biofilm. Furthermore, measurements of growth rate-related parameters for P. putida showed reduced rRNA content and cell size (relative to that in a batch culture), indicating that the P. putida population was not degrading toluene at a maximal rate in the biofilm environment. Assuming that the rRNA content reflected the cellular activity, a lower toluene degradation rate for P. putida present in the biofilm could be estimated. This calculation indicated that P. putida was responsible for a significant part (65%) of the toluene degraded by the entire community.