|
Post by jeany on Nov 11, 2009 18:31:48 GMT -5
www.apsnet.org/phyto/SEARCH/1987/Phyto77_327.aspVIEW ARTICLE Ecology and Epidemiology Recovery of Fungi and Arthropods from Sclerotia of Sclerotinia sclerotiorum in Quebec Muck Soils. Osama Anas, Graduate student, Department of Plant Science, Macdonald College of McGill University, Ste-Anne-de-Bellevue, Quebec, Canada H9X 1C0; R. D. Reeleder, Assistant professor, Department of Plant Science, Macdonald College of McGill University, Ste-Anne-de-Bellevue, Quebec, Canada H9X 1C0. Phytopathology 77:327-331. Accepted for publication 3 July 1986. Copyright 1987 The American Phytopathological Society. DOI: 10.1094/Phyto-77-327. Baiting samples of muck soil from Quebec with sclerotia of Sclerotinia sclerotiorum revealed that Trichoderma, Gliocladium, Penicillium, Sporidesmium, Rhizopus, Myxomycetes, Bradysia (dark-winged fungus gnat), and Onychiurus sp. (springtails. Order Collembola) were present in the soils. The number of larvae of Bradysia was positively correlated with low soil pH, high levels of organic matter, and high levels of nitrate in the soil. There was no correlation between fungi or Onychiurus sp. recovered and any of the above soil parameters. In in vitro tests, sclerotia damaged by the feeding of the larvae of Bradysia had levels of mycelial germination of 0–30%, whereas undamaged sclerotia germinated at a rate of 95%. When sclerotia were buried at different depths in soil, and larvae or adults of Bradysia were placed on the soil surface, predation of sclerotia was greatest in the top 2 cm of soil. The larvae were recovered from as deep as 9 cm in the soil. the rhizopus brings in the (thrips, for me) collembola, and fungus gnats as well as those parasitic wasps i use to get.
Baiting samples of muck soil from Quebec with sclerotia of Sclerotinia sclerotiorum revealed that Trichoderma, Gliocladium, Penicillium, Sporidesmium, Rhizopus, Myxomycetes, Bradysia (dark-winged fungus gnat), and Onychiurus sp. (springtails. Order Collembola) were present in the soils. So...there are the Fungus Gnats!... and they are 'picking up' the fungi, the Rhizopus? and when they land on our food and are in our environment, that's how the fungus infects us for example? ..hmm..Kamster?..Jo?? Thanks, Ruth! Good research..thanks to all of you too! What a great team! Jeany
|
|
|
Post by fritolay66 on Nov 11, 2009 21:50:51 GMT -5
Update on my petri. Wow. Almost the entire dish is covered in mould. It is quite dense and it growing up the sides. its about half way up. Only a small portion of the petri is not yet covered in this stuff.
To describe the contents as I have no digital camera. The main portion of the petri is a fuzzy white fluff of stuff. Up the sides of the container are most definately spores of a greenish or greyish nature with white powder on top of them. The spores are clumped together but still identifiable as seperate entities. Approx. 6 to a cluster. There also seems to be either greenish or greyish tones in the white fluff. The fluff is not powdered like the spores are. The petri samples reverse is pale yellow.
What does reverse mean? Let me take Toni's black speck for example. One side is opaque and the other is black. For a mold sample, the top side is one color and the bottom is another. The reverse. The reverse also helps in identification of a species.
If this is what is coming from our bodies, it isn't any surprise that so many of us are developing cancer and dying. My heart and love go out to Suebe, Curly, and Roberta Louise.
Frito
|
|
|
Post by fritolay66 on Nov 12, 2009 0:08:26 GMT -5
Okay everybody!!!
I think I may have found an identity to our spheres. They are referred to as globulose Hulle cells. I know rhizopus has been talked about and I haven't been able to correlate spheres with that one. But Hulle cells are associated with many species of Aspergillus. I think our spheres are Hulle cells.
Frito
|
|
|
Post by fritolay66 on Nov 12, 2009 0:20:09 GMT -5
Terminology:
Hulle cell:
A specialized multinucleate cell that originates from a nest-like aggregation of hyphae during sexual development. Hulle cells serve as nurse cells to the developing cleistothecium. Note that "Hulle" is properly written with an umlaut character on the "u".
cleistothecium:
A completely closed fruiting body formed by some fungi of the Ascomycota, containing asci.
|
|
|
Post by fritolay66 on Nov 12, 2009 0:42:52 GMT -5
|
|
|
Post by Sidney on Nov 12, 2009 1:41:23 GMT -5
Great work, Frito. So glad you're garden is growing so well.
Is this not the most amazing thing yet? I urge everyone to please buy the mold kit so we can all compare.
Finally, I feel we're making some good progress.
|
|
|
Post by bannanny on Nov 12, 2009 1:46:16 GMT -5
Thanks frito... I looked at it today and you're right. The moisture seems like it's slowly starting to dissipate now. I put it back inside the baggie too. Don't worry about my thinking problem ok? It's happened before and I'm sure it'll pass again like it always does... but thanks for your concern my friend. Yeah, I was seeing Dr. Hildy, but I couldn't make the drive any longer. She's about 85 miles from me. I'm taking lots of stuff tho... NAC, VitC, Calm Magnesium, L-Carnitine, Probiotics, B12, Folic Acid, Milk Thistle, Hysopp, CoQ10, MSM, Fish Oil, Cats Claw, and Wormwood. I just started another detox the other day too.
Thanks too for your tin foil suggestion toni (and about the B vits which I take.) What I did was rub my hands out onto a blank piece of white paper (the kind you use in a printer) and then poured it into the dish from there. When I can get another kit tho (it's a bad month) I'm just gonna put my hair in it, some eye gunk, and maybe some sputum, and leave the gel alone for now. I started washing my hands with a foam antibacterial soap, then I put gold bond medicated powder on, then 2 pairs of vinyl gloves on. It's been working pretty well at keeping the goo from oozing. Latex gloves always turned yellow and got goopy too, but these vinyl ones seem to be ok, so that's a good thing. I can type on my keyboard and hold the mouse now without my fingers and hand sticking to them. Only problem now is I can't think!
Like now... I have no clue what producing pre spores instead of sclerotia even means ruthie. Anyway, mine's sitting on top of the microwave inside the baggie that came with the kit. It gets sunlight, but not direct sunlight. I'm wondering... I didn't use the lid as the dish and the dish as the lid did I? The lid fits over the dish, not inside of it, right? Sounds like a stupid question I know. But sometimes lids aren't always on the outside and I was just trying to figure out why there's moisture. Probably wouldn't matter much anyway tho. But my lid fits real loose over the dish, does yours?
So what's the deal with Rhizopus infecting adhesive bandages that were used? I'm trying to remember if any were used on me the times I've been hospitalized. Only times I can think of were when I had spinal surgery and shoulder surgery. How bout you guys? I've had the springtail (collembola) connection too.
So jeany, you think we're being infected with the Rhizopus aspect of morgs via insects like FG, collembola, etc. that have picked it up? If we are, maybe it works as a catalyst to make whatever the crux of morgs is to start producing in the body? I dunno... just trying to think! It sure was nice talkin to you and kam on the phone tonight too... til my stupid battery died again! You made me feel good tho... I love ya's!
Thanks for the "reverse" explanation too frito... you guys sure know your stuff! I wish you had a camera... can you borrow one from somebody? How long did it take you now to see anything? Your description reminded me of those whitish/grey cottony clumps of stuff that were on my back door window.
HOLY TOLEDO!!!!! I just went to the kitchen to get a cig (I keep 'em in the freezer) and decided to take a quick look at my dish again. On top of my sample now are 2 separate round piles of pale green fuzz!!!! Be right back... I'll try to get a pic!
|
|
|
Post by bannanny on Nov 12, 2009 2:04:18 GMT -5
Ok... I tried to get it with the flash on first and that didn't work at all. So I turned it off and I think I got it good enough for you to able to make it out... Can you see the green stuff on top of the sample? Crazy eh? You can tell the moisture isn't covering the inside lid as much now too. Man, I wish the picture showed it as good as I can see it. I'll try and get a better pic during the day tomorrow. I got a feeling this is gonna make me set my scope up now... ooooh, I wish I didn't feel so much like a chicken. I forgot to say anything about those Hulle cells frito... got too excited! So they're associated with asperguillis eh? I think asperguillis is part of this mix too... don't you guys? love ya's ~~ bannannas
|
|
|
Post by toni on Nov 12, 2009 7:43:19 GMT -5
Frito,
Rhizopus grows incredibly fast, and it's texture is that of like cotton candy (as per the articles). Yours sounds like the rest of ours too, imho. I'm so glad we're all doing this for comparison's.
Bannanny,
Mine too (the lid) was covered in condensation like yours. I believe that is very normal when something takes hold and begins to grow like that (in such a small place as the petri dish).
And you'd asked about the lids. Yes, they're just very loosely set on actually - the Pro Lab ones. More so, like just a cover that sits on top with sides.
|
|
|
Post by jeany on Nov 12, 2009 9:10:59 GMT -5
Terminology: Hulle cell: A specialized multinucleate cell that originates from a nest-like aggregation of hyphae during sexual development. Hulle cells serve as nurse cells to the developing cleistothecium. Note that "Hulle" is properly written with an umlaut character on the "u". cleistothecium: A completely closed fruiting body formed by some fungi of the Ascomycota, containing asci. Hey all..I thought it might be a good idea to add the Hülle Cell pic here too: Thanks, again Frito! Jeany
|
|
|
Post by fritolay66 on Nov 12, 2009 9:39:27 GMT -5
Jeany, I posted a link to a very long history paper on fungal infections. Within that text are even better pictures of the Hulle Cells. After looking at those, and at my slides, I definately am seeing these. I can't get the picture to save, mmm, just thought of a way I could try to get that illustration. I'll work on that. Toni, Here are some links to kind of help you with the 'crux" you have going through your mind. Mold is capable of shutting down the immune system by the mycotoxins it produces and can through the skin as well. Some don't need anything from the substrate it invades to produce these toxins. With immune system shutdown, one would then become suseptable to multiple bacterial infections. Very similar to the AIDS patients. Add in symbiotic molds, and you might get a lichen. Add those to the cornybacterium (Diptheria) and you get resistent dermal lesions. en.wikipedia.org/wiki/Trichotheceneen.wikipedia.org/wiki/Stachybotrysen.wikipedia.org/wiki/SatratoxinMy thoughts on how Lyme fits in this. Anyone familar with the issues of Lyme know that it is not only being vectored by ticks. Also, it can be transferred from human to human. Almost like an STD. The body with an intact immune system MAY be able to hold its own, but compromise, especially by mold, would allow it to flourish and mask its presence. Dr. Schaller also states that mold will compound the issues of Lyme. I suspect with the fungal and mycotoxin issue, that Lyme would not be able to be effectively targeted. Chronic, irretractable Lyme and company. Also the mycotoxin links I gave you, you will be able to read about the toxins interference in protein folding and RNA and DNA. We have suspected this all along. Frito
|
|
|
Post by jeany on Nov 12, 2009 9:46:36 GMT -5
Hi Frito..I couldn't open the file..that's why I didn't see the pictures.. sorry, only wanted to contribute a bit. Jeany
|
|
|
Post by fritolay66 on Nov 12, 2009 10:05:45 GMT -5
|
|
|
Post by fritolay66 on Nov 12, 2009 10:07:11 GMT -5
Sorry? For what? Was I snippy, I apologize if I was but I wasn't trying to be. Contribute dear girl all you want. You take over, I have to go to work, yet again.
|
|
|
Post by fritolay66 on Nov 12, 2009 10:09:27 GMT -5
|
|
|
Post by violet on Nov 12, 2009 10:24:38 GMT -5
I am in total awe of the collaboration and scientific findings of you all. We're very grateful for your persistence!!! Very, very grateful!
|
|
|
Post by violet on Nov 12, 2009 10:30:24 GMT -5
Jeany, I posted a link to a very long history paper on fungal infections. Within that text are even better pictures of the Hulle Cells. After looking at those, and at my slides, I definately am seeing these. I can't get the picture to save, mmm, just thought of a way I could try to get that illustration. I'll work on that. Toni, Here are some links to kind of help you with the 'crux" you have going through your mind. Mold is capable of shutting down the immune system by the mycotoxins it produces and can through the skin as well. Some don't need anything from the substrate it invades to produce these toxins. With immune system shutdown, one would then become suseptable to multiple bacterial infections. Very similar to the AIDS patients. Add in symbiotic molds, and you might get a lichen. Add those to the cornybacterium (Diptheria) and you get resistent dermal lesions. en.wikipedia.org/wiki/Trichotheceneen.wikipedia.org/wiki/Stachybotrysen.wikipedia.org/wiki/SatratoxinMy thoughts on how Lyme fits in this. Anyone familar with the issues of Lyme know that it is not only being vectored by ticks. Also, it can be transferred from human to human. Almost like an STD. The body with an intact immune system MAY be able to hold its own, but compromise, especially by mold, would allow it to flourish and mask its presence. Dr. Schaller also states that mold will compound the issues of Lyme. I suspect with the fungal and mycotoxin issue, that Lyme would not be able to be effectively targeted. Chronic, irretractable Lyme and company. Also the mycotoxin links I gave you, you will be able to read about the toxins interference in protein folding and RNA and DNA. We have suspected this all along. Frito When you mentioned lichen, Frito, it made me think of one time when I had gone to the dentist for a routine checkup, and the hygienist showed me in a mirror the roof of my mouth which had a beautiful design on it (that really shocked me!). Neither she nor the dentist knew what it was, and I was wondering how in the world whatever it was had gotten in there. It was roundish and perfectly symmetrical and intricate in its design, very much like you would see if you looked into a kaleidoscope. I do remember the roof of my mouth having itched at one point. The hygienist said that whatever it was was in the almost-healed stage. My gosh, what in the world is it that we have?!??!?!??!!!!
|
|
|
Post by jeany on Nov 12, 2009 11:02:40 GMT -5
Hey Frito..it was an 'Opera thing'..opened it with IE just fine..thanks again.. :)Jeany
|
|
|
Post by toni on Nov 12, 2009 11:41:24 GMT -5
Thanks Frito for the links.
Yes mold sure can affect the immune.
I'm still gravitating towards Bt though as the immune problem.
|
|
|
Post by Sidney on Nov 12, 2009 13:55:22 GMT -5
As Violet mentioned, I am in awe too. Thanks to each of you for contributing incredible information.
A few things I would like to touch on as follows:
Bananny mentioned the green in her petri dish. I had never noticed green until a few minutes ago when I took my two petri dishes to the big window in the kitchen and examined the dishes with a magnifying glass. Such a colorful collection in these dishes, including green. I would describe it as olivaceous specks along with black appearing specks in that part of the dish. They have a gritty-gravelly appearance. Makes me think of Lichen I've seen growing on old branches or logs. Somewhat elevated.
Toni asked if I have the white fluff and yes, I do. It's pictured in the images she posted for me, first page of this thread.
The colonies are collapsing now, in particular the colonies in the first petri dish I started October 23.
The second petri dish has more gray mold than any other color, but there's a large area of white along with darker gray colonies.
The yellow colonies are a brilliant lemon-yellow.
Frito, when you wrote about having fungus in your ears at age nineteen I wonder if you ever heard sort of scratching sounds and felt scratching sensations. I also wonder if you ever heard the "snap-crackle-pop" sounds or a high pitched whine, or dull roar that I heard in years past.
Funny thing, the last two days when I showered I've poured Peroxide in my ears, one ear at a time, tilted my head carefully and waited for the familiar sizzle and the sensation that always makes me shudder. Don't know how else to describe the sensation and sounds of sizzling.
The past two days I can't get the peroxide to penetrate whatever seems to be blocking it. I get no sizzle at all, no "boiling" sound, or the sensation that gives my body a serious shudder. I've also noticed my balance seems to be a little "off" again. Lord, please, no Vertigo!
|
|