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Post by toni on Nov 5, 2009 13:23:22 GMT -5
I'm glad you mentioned Ruth about the fibers too.
I'm going to place some of those in the petri dish too.
I just got mine all set up.
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Post by ruth on Nov 5, 2009 13:33:43 GMT -5
anthill is going to try to merge this thread with sidneys' thread by toni
kammie,
i should put the culture dish in a box to block the light?
i have baseboard heaters. should i put by the heater?
thanks
toni, how and where are you putting your dish?
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Post by toni on Nov 5, 2009 13:59:41 GMT -5
Ruth,
I was going to put it in a spare bedroom, (where it wouldn't be disturbed).
I've got the a/c and heat vent in the wall closed, so the temp will probably remain about 60-70 in there.
It's sort of dark in there, and actually....I'm not sure where would be the best place.
Do you think the petri dish needs (no light) from the sunshine outside? Or would it be better if I just keep the blinds closed and it got no lighting at all?
I could put it in a closet too. I really don't know where the best place is?
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Post by Sidney on Nov 5, 2009 14:11:43 GMT -5
in the prolab kit, the the media is liquid. it needs one hour to set. the problem i have is many of us have had the doctor order cultures and without results.my contention here is that the proper food source for M is not included in all culture medias. and that M can easily revert to a liquid form without the proper food for it to do its cycle. as sid said.......these are specific for molds....i will have faith and not put any other food source into the new petri dish............plus i am making sure i have the red, blue, green, brown, black colored fibers put in the dish there is thick white cottony growth covering about 2/3 area of the 1st dish. it makes me sick to see it grow so abundant. it is what i have seen for years with only sparse representation until now. good Lord the surprising thing is one of the oat flakes has been moved about an inch from where it started and is becoming amber goo. i can hardly wait to see what else is surprising Ruth has it right. The Pro-Labs kit is a very non-complex kit. The culture medium is in a small plastic bottle with a screw on lid. DO NOT SHAKE THE BOTTLE. Just pour the contents into the Petri dish making sure the dish is on a level surface. Put the lid on it and put it away. This is NOT a screw on lid for the petri dish. It simply sets on the dish, so please pick it up carefully, place it where you plan to view it, and leave it alone. I placed my dish in a kitchen cabinet with glass doors. There is nothing on the instructions to indicate the petri dish needs to be kept in a dark place. Read Ruth's instructions on page one. She has typed them from the instruction sheet that came with the kit. Thanks, Ruth. My culture number two is growing the white fluff.
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Post by ruth on Nov 5, 2009 14:13:54 GMT -5
toni, i had the lid off while the media was setting up.
it had many spores already just from the air.
i got the feather included in this one, too.
i guess till we hear from kammie and sid about where and how, i'll put it in a box close to the heater, although i don't always have it on. i wonder about setting it on the water heater?
well, i gotta get to work, so i'll check this when i get home.
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Post by toni on Nov 5, 2009 14:25:12 GMT -5
Thanks Sid and Ruth.
Yes the Pro Lab Kit is incredibly easy. ( I was concerned it would have been all complicated) and I'm horrible with directions, haha
Like you said Sid...don't shake the little bottle, just squirt it in the dish and that's it. Wait one hour for the liquid to turn to gel, then put in your specimens and put the covering back on the top.
Ruth, I know what you mean about "stuff floating in the air".
When I was letting the liquid solution set for the one hour ( I put the covering back on) for that hour, just in case anything from in the air got into it. I wanted to be very careful as to not allow anything from my environment (air) inside. So I immediately covered it back, and only took off the lid/covering while placing each specimen into the gel/gelatin stuff.
I put it in the spare room on the dresser. I'll open the blinds in there just so it gets a hint of daytime light, and other than that, I'll "set it and forget it" hahha (like that commerical) and in a few days, I'll go take a peekie at it.
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Post by Sidney on Nov 5, 2009 17:29:30 GMT -5
I wouldn't place it in a dark place, not by a heater, not on a hot water tank.
I would NOT leave the dish uncovered while the gel medium is setting up.
Have the lip off the dish only while adding the specimens. That way you are assured of not getting any airborne contaminants....hopefully.
Again, I placed my specimens in the dish with the bathroom door closed and no air currents from the ceiling heat and air source.
We need to do these tests as "carefully" as possible with each person following the exact same process.
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Post by ruth on Nov 5, 2009 23:06:10 GMT -5
okay, i'll just keep it in my laboratory room.
sid, are you getting the white cottony growth?
it looks like the amber gell on the flake is turning dark and with just my eyes, it looks like black mycelia coming on.
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Post by Sidney on Nov 6, 2009 0:26:05 GMT -5
Ruth, you are really doing some incredible work here. I so want answers, just as we all do. No point in going into the frustrations and sufferings we've all encountered and endured, but haven't any of you noticed this?
I use glass lab slides for facial specimens. Unless I cover the specimens with another slide and tape all four edges securely, I find some of the specimens will actually move to the edge of the glass slide, will move completely OFF the slide, and I've even found bits of my skin many inches away from the slides on which they were originally placed. Now, how can that be? I don't see a "critters" moving them, so I now have to conclude they are actually moving under their own "steam." Sorry, all this stuff just gags me. I love a good crime drama like CSI, but have to confess that it's much more graphic than I can bear, so I just close my eyes until the autopsy is completed or the bloody body is covered or placed in a body bag.
No guts, no glory.
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Post by Sidney on Nov 6, 2009 0:49:36 GMT -5
okay, i'll just keep it in my laboratory room. sid, are you getting the white cottony growth? it looks like the amber gell on the flake is turning dark and with just my eyes, it looks like black mycelia coming on. My first culture grew a strange assortment. As I wrote in the beginning, my doctor said he saw five different species growing. Bearing in mind he isn't a Mycologist he could have been wrong. What I saw was different shades of gray, white and black. One of the stranger growths was a pale yellow, flat in appearance almost dime size something or other that reminded me of a Sand Dollar in that it wasn't perfectly smooth. Within days it had thinned out a bit, spread a bit, developed a black spot on top. The spot in the center of the black spot was lighter, but the outward edges of the black spot eventually became gray. I just looked at both the petri dishes. More white fluffy growth on several of the specemins now.
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Post by ruth on Nov 6, 2009 1:40:55 GMT -5
check this lady out, maybe you've seen her pics before. my God, i am not as bad as she is. my lesions are not large like that www.morgellons-research.org/morgellons/under "sufferers pictures" when the second dish is ready.........i'm sending it in for identification. molds are too complicated. i'll get an expert to name it. sid, toni, are you sending yours' in to the lab for id also?
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Post by kammy on Nov 6, 2009 7:25:39 GMT -5
kammie, i should put the culture dish in a box to block the light? i have baseboard heaters. should i put by the heater? thanks No, don't put the dishes next to heaters (unless they are in a box) because it will cause the dishes to dry out prematurely. If you just put them in a shoe or mailing box and turn the dish upside down, that should be fine. (unless your dish has runny solution in it - then don't turn it upside down) They say to put the box up high - (heat rises) like on the top of your refrigerator. You guys are doing great!
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Post by Sidney on Nov 6, 2009 11:39:31 GMT -5
I've seen these pictures and they have to be the worst ever. Heartbreaking and makes me ashamed for ever complaining. Thank you, Ruth. It's definitely a reminder to count our blessings.
That's Marc Neumann's site and his images are remarkable.
As to sending a Pro-Lab kit to be analyzed, yes, I plan to do that. I want to do a sputum culture first, see if it grows anythng, then will begin another petri dish using skin specimens. After I see initial growth I'll send it in with a check for $30.
My second petri dish is now growing white fluff on four different specimens. I started this one last Monday night.
Kammy, you and Jeany have been extremely helpful and thank you so much.
Again, please pick up some of these kits and just do it.
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Post by violet on Nov 6, 2009 12:22:08 GMT -5
grrr still a typo...what the heck is "negatate"!!!!!!!!!!! I meant negate Cousin to "conversate"?
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Post by ruth on Nov 6, 2009 21:05:44 GMT -5
i have nothing but the white mold growing in the 1st petri dish. the samples were from deep within lesions. i know if the oat flake was contaminated, the growth would not have been so quick. the second dish should grow the same without the flakes.
many pieces of the slime mold petals were used.
the white fibers are starting to come out of the dish. i had to tape it shut. it is what i recognize......
the 2nd dish is starting to form the mold. it is invisible but with the right light, i see it branching and forming. i have already taped it shut.
the 1st plate is so dense, i am unable to get a picture. it is producing a gazillion spores. the whole dish is full now.
i can't even get a pic of the whole dish, like toni does.
it is really giving me the creeps........seeing what it is apart from me............so creepy!!!!!!!
today, i put the culture dishes in a box without a lid and sit it on the refrigerator. it seems to like it there.
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Post by kammy on Nov 6, 2009 21:56:28 GMT -5
I will, Sid, the next time I get in Lowe's direction... this is important to us all.
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Post by ruth on Nov 6, 2009 23:09:29 GMT -5
taken thru the lid of the first culture plate
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Post by Sidney on Nov 7, 2009 0:52:38 GMT -5
I posted a few locations where the Pro-Lab kits may be purchased. Posted them at the thread Toni started for me. SWal-Mart appears to carry them now, as well as Target, but I am NOT sure about Target. They're less expensive at Amazon.com too and fast delivery.
I so hope something comes from this. Obviously self-collected isn't worth squat, but we're all desperate for help and the more who do the self-test the more we'll learn. At least, I hope that is the case.
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Post by jeany on Nov 7, 2009 8:08:24 GMT -5
Great shots, Ruth! Looks typical...with the conidia forming. We have seen these many times. It matches the photos Kam has taken. How many days growth is it?
So..you think it is a slime mold? Could possibly be, if you think how many people have reported about a creepy crawling feeling.I had that on my scalp.It was like something was spreading and when I touched my hair/head on that spot it felt damp...
Ruth, were you able to identify it yet?
It's great how we're getting together to find similarities..Are we finally getting somewhere?
Jeany
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Post by kammy on Nov 7, 2009 11:01:57 GMT -5
Be sure to keep the lid on your dish after putting in the agar mix, and to only open them when you are photographing or studying them.
Also, be sure to wash your hands and under your fingernails good if you are using your fingers to collect your specimens.
The less variables - the better.
And, Ruth great shots thru the lid.
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