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Post by Meeow on Feb 26, 2006 20:12:37 GMT -5
Who here has mercury in their mouths for fillings....? Another point to ponder, perhaps? Pseudomonas flourescens - seems to be likely due to the nature of this beast , hm
May the force be with you
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Post by LONDON as a guest on Feb 26, 2006 21:25:22 GMT -5
Reduction of natural adenovirus tropism to mouse liver by fiber-shaft exchange in combination with both CAR- and alphav integrin-binding ablation.
Division of Cellular and Gene Therapy Products. National Institute of Health Sciences, Tokyo 158-8501, Japan.
The primary receptor, the coxsackievirus and adenovirus receptor (CAR), and the secondary receptor, alphav integrins, are the tropism determinants of adenovirus (Ad) type 5. Inhibition of the interaction of both the fiber with CAR and the penton base with the alphav integrin appears to be crucial to the development of targeted Ad vectors, which specifically transduce a given cell population. In this study, we developed Ad vectors with ablation of both CAR and alphav integrin binding by mutating the fiber knob and the RGD motif of the penton base. We also replaced the fiber shaft domain with that derived from Ad type 35. High transduction efficiency in the mouse liver was suppressed approximately 130- to 270-fold by intravenous administration of the double-mutant Ad vectors, which mutated two domains each of the fiber knob and shaft and the RGD motif of the penton base compared with those of conventional Ad vectors (type 5). Most significantly, the triple-mutant Ad vector containing the fiber knob with ablation of CAR binding ability, the fiber shaft of Ad type 35, and the penton base with a deletion of the RGD motif mediated a >30,000-fold lower level of mouse liver transduction than the conventional Ad vectors. This triple-mutant Ad vector also mediated reduced transduction in other organs (the spleen, kidney, heart, and lung). Viral DNA analysis showed that systemically delivered triple-mutant Ad vector was primarily taken up by liver nonparenchymal cells and that most viral DNAs were easily degraded, resulting in little gene expression in the liver. These results suggest that the fiber knob, fiber shaft, and RGD motif of the penton base each plays an important role in Ad vector-mediated transduction to the mouse liver and that the triple-mutant Ad vector exhibits little tropism to any organs and appears to be a fundamental vector for targeted Ad vectors.
London
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Post by LONDON as a guest on Feb 26, 2006 21:27:45 GMT -5
Accumulation of infectious mutants in stocks during the propagation of fiber-modified recombinant adenoviruses.
In infected cells, replication errors during viral proliferation generate mutations in adenoviruses (Ads), and the mutant Ads proliferate and evolve in the intracellular environment. Genetically fiber-modified recombinant Ads (rAd variants) were generated, by modification of the fiber gene, for therapeutic applications in host cells that lack or express reduced levels of the Coxsackievirus and adenovirus receptor. To assess the genetic modifications of rAd variants that might induce the instability of Ad virions, we examined the frequencies of mutants that accumulated in propagated stocks. Seven of 41 lines of Ad variants generated mutants in the stocks and all mutants were infectious. Moreover, all the mutations occurred in the modified region that had been added at the 3' end of the fiber gene. Our results show that some genetic modifications at the carboxyl terminus of Ad fiber protein lead to the instability of Ad virions.
London
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Post by LONDON as a guest on Feb 26, 2006 21:48:45 GMT -5
Fiber-mosaic adenovirus as a novel approach to design genetically modified adenoviral vectors.
adenovirus (Ad) capsid is one of the successful strategies to achieve viral retargeting. However, it has been widely recognized that structural constraints imposed by viral proteins limit the number and nature of incorporated targeting ligands and often hamper viral propagation. To address this issue, we propose a genetic fiber-mosaic virus (having two distinct fibers in one viral particle) as a means to facilitate fiber modifications. Fiber-mosaic virus having tandem fibers: a wild type (wt) fiber and second adjunctive fiber, will utilize natural viral entry for the conventional propagation of the vectors whereas, adjunctive fiber will serve multiple potential purposes such as targeting, purification, or imaging of viral particles via genetic incorporation of the corresponding functional moieties. We generated the mosaic adenovirus vector encoding two fibers: wild-type and adjunctive fiber--Fiber-Fibritin (FF) and confirmed incorporation of FF in the mosaic viral particles. We investigated binding specificity of the mosaic virus and the possible interference of the two fibers during virus life cycle. Fiber-mosaic Ad attained new binding properties provided by the second fiber, while preserving the binding ability attributed to the wt fiber. Our results suggest that the two fibers being presented and structurally separated on the viral particle may also function separately as binding counterparts for virus attachment. Therefore, the mosaic setting will allow more flexibility in Ad retargeting approaches.
London
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Post by LONDON as a guest on Feb 26, 2006 21:53:22 GMT -5
Trypanosoma cruzi surface mucins: host-dependent coat diversity.
The surface of the protozoan parasite Trypanosoma cruzi is covered in mucins, which contribute to parasite protection and to the establishment of a persistent infection. Their importance is highlighted by the fact that the approximately 850 mucin-encoding genes comprise approximately 1% of the parasite genome and approximately 6% of all predicted T. cruzi genes. The coordinate expression of a large repertoire of mucins containing variable regions in the mammal-dwelling stages of the T. cruzi life cycle suggests a possible strategy to thwart the host immune response. Here, we discuss the expression profiling of T. cruzi mucins, the mechanisms leading to the acquisition of mucin diversity and the possible consequences of a mosaic surface coat in the interplay between parasite and host.
London ****Notice the word: mucins; it will come up again.
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Post by LONDON as a guest on Feb 26, 2006 22:01:41 GMT -5
Introduction of a Carboxyl Group in the First Transmembrane Helix of Escherichia coli F1Fo ATPase Subunit c and Cytoplasmic pH Regulation This is a 7 page pdf file............FYI tinyurl.com/qglu3PS: Carboxyl is what I was telling you theuy put into diapers, women's Fem products and also some disin fectants.......and something to do w/ carpet too London
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Post by London to UkGuy on Feb 26, 2006 22:13:29 GMT -5
Hey Uk guy! Good to hear from you......it really is! Yes Pseudomonias, at leat I've seen a lot of articles on it. As well as Esters and one I posted about last week, uh..Lysophospholipase_carbox ylesterase......Here's one for you on Particles and Pseudo's..... ACCUMULATION OF HEAVY METALS BY A BACTERIUM ISOLATED FROM ELECTROPLATING EFFLUENT tinyurl.com/n6n57
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Post by skytroll on Feb 26, 2006 22:25:43 GMT -5
Junebook,
In the Morgellon literature it talks of this, cutting off the heads with a razor or something to that affect, in "a letter to Browne". They said it affects the muscles, and I think the worm was white, but the head was black.
London, Ok, I see what you mean by the anthrax and BT, just the plasmids are different and can pick each others up. You know when they first started spraying for thoses gypsy moths, here they used BTK. Maybe that BTK is synonymous with Anthrax, then they would be the same.
I think it is Kurstaki, something like that will look that up. I will see if I can find that paper the county sent me, back in 2000.
Also, the adenovirus thing above, is to be used as a vector for gene delivery to cystic fibrosis patients, to replace the bad fibers, I believe. But, the whole idea of using a virus for gene delivery is quite dangerous, because then the patient will end up with the virus, unless there is a shut off switch of the virus. The gene delivery, I believe is to repair the epithelial cell of cystic fibrosis patients.
That was a heavy read, but, I think I saw through the genetic lingo. They tried something like this on a young boy, and he died because the virus killed him. Back in late 90s I believe. It never got to the gene.
Good discussions going on here folks. We have to weed em out........
Got some physics and unknown geology science readings that haven't been put in print, until recently. Something other than biology for a while. Let you know what my feeble brain picks up, expecially the particle matter and such.
skytroll
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Post by LONDON as a guest on Feb 26, 2006 22:38:00 GMT -5
Hey Sky, yes, I hope you can find it. I've got to run until tomorrow afternoon
but I plan on posting my big trypansome paper, and a whole lot of bad stuff going on.....
i.e. where they literally tell the Co. workers to tell lies, the( Also) artificial intelligence,
The guilty universities ( lots in London but a biggy in Japan).
And yes, June, I remember you writing about the worm too.
Hey forgot to tell you.......first , does anyone know what color the Diamond Back Moth is....
it does not happen to be black and white does it? I heard.......( I think this is right.....)
If we say like go sit on top of a black trashbag, then white fibers will come to us and vice-versa......
but I don't know,,,,,I;m out of trashbags! hehehe just kidding, but I have not tried it.
Later All,
London~
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Post by skytroll on Feb 26, 2006 22:42:12 GMT -5
T rypanosoma cruzi surface mucins: host-dependent coat diversity. The surface of the protozoan parasite Trypanosoma cruzi is covered in mucins, which contribute to parasite protection and to the establishment of a persistent infection. Their importance is highlighted by the fact that the approximately 850 mucin-encoding genes comprise approximately 1% of the parasite genome and approximately 6% of all predicted T. cruzi genes. The coordinate expression of a large repertoire of mucins containing variable regions in the mammal-dwelling stages of the T. cruzi life cycle suggests a possible strategy to thwart the host immune response. Here, we discuss the expression profiling of T. cruzi mucins, the mechanisms leading to the acquisition of mucin diversity and the possible consequences of a mosaic surface coat in the interplay between parasite and host. London ****Notice the word: mucins; it will come up again. So Chagas Disease, or trypanasoma can form a mucin diversity, and interacts with host. I read how this works almost like LYmes, is autoimmune, keeps going on over and over and all the stages are present. Will find writeup on that. Denegri Web site. www.cdfound.to.it/HTML/trip1.htmLook at last picture and click on it to enlarge it. It describes the treatment and is endemic in Latin America. skytroll
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Post by skytroll on Feb 26, 2006 22:44:20 GMT -5
thanks London for all you do.
Skytroll
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Post by skytroll on Feb 27, 2006 0:45:18 GMT -5
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Post by lizzin portlannd on Feb 27, 2006 7:46:24 GMT -5
I am new at responding so be patient with me..I suffer with you all from morgellons and all those gross odditys that come with it...I have them all....but what caught my eye while going through this subject is the mention of flukes and Iam becoming more and more convinced some how they tie to this illness and heres why...i went to one of those skin beauty places to have my pores vacumed knowing full well something awful might come out of the lumpy mass on my chin and it did....ofcourse they asked me to leave and see a docot or betteryet go to sharper images where they sell those pore vacumes which i did and i suggest this to all,as these suck things out you cant get wwith tweezerswhile vacumeing one of those big painfull masses you know when your getting the bst of it cause it starts blleding like hell and it hurts like hell....at first you will think you got a bunch of big blood clots..... but put them in water and youll see they have substance as the blood wahes off...the fist one i put in a test tube where it looking almost like a leechfirst day being flourestaant reddish pink then turning to brown around edges the feeling and constancy of a mushrrom cooked i watched as it isucked onto the side of the glass...they looked almost like leacheskinda of dragon like head at the top with a small antenna coning out top of had wwwith a ball at the end...as we contined to watch we noticed what appeared to be babys coming out of the center of the animal which is also where there is that infamous circle or broken circcle that we see at the head of our lesions or sores sometimes with a big hole in the center....i beleive maybe these tissue fluke lay fat underskin horizontly branching out from each side of hole which is where i beleive it has babys, breaths, poos, as well as pushes out all sorts of other things we are seeing....the lilttle ones looed like little sperms always a small ball with a tail dragging behindg . also saw those black specks emerge from thet same hole(could this be their waste?) later on however i saw with a witness these serpant like flukes expell right out the end of their snout or maybe mouth a huge long fiber atleast flukes 2-3 inches ( consistancy and thickness of a cat whisker) i have read where fluke infectionns- travel all over and will end up those mussy lummps under skin... this could expain if they lye horizontal under skin why there so hard to get out and w hy the lesions don'r heal...we think when we pull the stuff out of the opening or hole whether it be a back sppeck or a tadple like creatue or white stuff or a wwwwwwwworm like thing that we aarere getting it when all we are doing is helping the tissue fluke expell whateever it had to expell.....l and occasionall injuring it as we dig around and make it bleed but defenatly not taaaakning out what has to come oput for lesion to heal and i would think that wiuld include all stray fibers as well...may have to be done sugally or that lesion is there to stay...noy everyone but the big long time monster lesions. there is also stuff that comes out that hole f that lookes like small cursds of cottage cheese. Since the i collected a couple of more from the toilet....you have to look close as they maybe all rolled up with other fecal matter but mine floated straight to the top...after rinsing it was so obvious a fluke but a little different variey, the body shaped shaped like flat lave narrowing down to a to the head and snout....thre was not an eyeball but circles where eyes would be...it had on the bottom of it body what looked like a flipper and the tail was long and ribbed just like lizzard tail....i sent that one in to have it come back as being a flukeof possible new origin...the ones that came out of the toilet were just missing the little antenna thing on head...my point is that out of these creaues comes alot of are mystery items...including are fiber...after reading up on them i also saw how juveveniles could look like those little pieces of grass you seem to find around the floor and so on...they can suck the calcium right out of your knee caps making you get the old knee replacement speech from doc...they clog up your circulatory as well as lymp system which i beleive has put me in a wheel chair/not to mention attacking lungs,brain heart skin,liver,and last but not least cause severe urnary incontinece....could they have a connectioni???I know theres still the liitle flys and glitter and many many other odditys not accounted for but could it be a start...sorry this is so long...comments would be appreaciated...good luck to you all i know it sucks so bad there are many days i can't even out of bed and going to the showere has even gotten tramantic as i never know what going fall out or emerge through my skin or what i will find the bathtub floor...
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Post by skytroll on Feb 27, 2006 8:37:32 GMT -5
Does it look similar to this? I think it does. tinyurl.com/g986pClick on the blue high lighted figs. Skytroll
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Post by questionhair1 on Feb 27, 2006 10:49:08 GMT -5
Does it look similar to this? I think it does. tinyurl.com/g986pClick on the blue high lighted figs. Skytroll I tried to see the photos, but they don't show as "clickable". The descriptions provided by Spec and Lizz and others are so fascinating. How I WISH there could be photos provided for examination and comparison. I can't describe to you all how excited I get when I see photos that match what I have found. Or, even when I see photos provided by different people of the SAME thing. Have you examined the photos at LymePhotos AND the photos of EverHopeful? see the matching star-shaped thing that is part of a parasitic cycle that is guessed at on the LymePhotos website (read the WHOLE website, it leads you through from photo to photo and then gives some theories along with a speculated life cycle) www.dpref.com/grains2.htmlwww.lymephotos.com/index.shtmlThe descriptions I've been reading, and the thoughts about them, are GREAT. I just wish we could see the photos too. Skytroll, I don't know why I can't click onto the photos of the link you've provided. The several people who have suggested that what is under our skin is larger than we can imagine and that the "stuff" we see is basically waste elimination, or intake, or breeding is a fascinating thought. A week or two? ago I stopped taking my vitamins, etc. I did stick to the Candida diet. It was the time of the full moon and it was also my "time of month" and one night I experienced the creepy crawly feeling of "slithering" under my scalp. It kept me awake. It was the first time I had experienced that - but fortunately I had already read about it here, so I wasn't as frightened as I would have been otherwise. I wondered if it was a) something(s) big or b) electrical impulses that made me feel like something was under there. Everytime people provide photos - people who don't know each other or are from different regions - and the photos MATCH is more proof that cannot be denied. Thank you for the descriptions and the thoughts about what the causes are. For Orion, and the stealth virus thoughts. I have printed out so many of those articles and read them over again - hoping for better understanding. In the meantime, I found speaking with Dr. Martin to be helpful and he recently suggested to take EH-101, which I bought from YK Flora ($23) - to me, it tastes the same as the A.C.E. ($39) - but I don't know what the difference is. Someone else (Ruth?) recently posted on the effect of supplements, and I think there was mention of pine extracts - it caught my attention since that is what the EH-101 is. My thought on the A.C.E. was that is a mineral supplement, I think there is mention of Utah's salt lake, etc. Anyway, sorry this is so long. Thank you all for your detailed descriptions - and I hope to see more photo albums - I think that is a POWERFUL tool - not only for discovery, but also for proof and explanation to people who don't understand. QH PS: I've started taking the vitamins, etc. again. With the recent threads on parasites controlling their host, I wondered if the "gag" feeling I get from taking vitamins is caused by parasites . . . strange thought, I know.
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Post by guest millenium on Feb 27, 2006 11:51:06 GMT -5
Lizzie,
what method do you use to get into those begger lesions? I too think a vacuum exptracotr is a god idea, but for those bigger areas, are you cutting into the lesions somehow?
I too have seen perhaps inch long pieces- maybe longer- of what looks like dried grayish green grass blades all around and I thought that was really what they are. Your is not the post to describe them.Has anyone looked at them under a scope?
I am about ready to take and open some lesions onmyown head I am so tried of this. I cannot get so far any doctor to do this. yet none of them will prescribe systemic meds for what they call a fungal scalp infection. I have large pillowy areas with massive movement underneath and branching out to ands from them. In the middle of these pillowy areas are crater like lesions.
I am sick of this.
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Post by guest millenium on Feb 27, 2006 11:52:28 GMT -5
Sorry, Lizz, for calling you Lizzie.
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Post by Mrs. Monk on Feb 27, 2006 12:37:42 GMT -5
when I first noticed this disease an entomologist was observing something that came out of my nose - he reluctantly said it was demodex but I've never heard of one that big. But he said there was a lot of grass, pollen, and other stuff in mynose that shouldn't be there. I can only guess that when the insects are first attracted to us, much of what is on them gets into us from them moving around and that might explain the grass.
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Post by guest millenium on Feb 27, 2006 13:43:22 GMT -5
I just read over my posts on this thread and I am dumbfounded.They look like someone who is either a young child has written them or someone who has 'problems'. No excuse here but lately I am forgetting how to spell things I have known how to spell all of my life, losing things, misunderstanding directions, transposing phone numbers and other figures and letters in words. All the time. It takes me a long time to correct my posts, and I try to, but sometimes I type out something thinking it is ok and then look at it later. sheesh. I never used to be this way.
Lizz's post is one of the most descriptive I have read in a long time. I so appreciate any and all posts here.
Lizz are you getting things out of your nose that look like slender worms all bound up in white mucus and clear stringy stuff? Do you have sensations that something is moving in your sinuses and post nasal "drip' sensations without the stuffed up nose? My nose is clear usually- not even a cold- but my sinuses behind my forehead seem full of something moving around at times. Definitely feels worse on damp days.
Great thread. Mil.
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Post by curry on Feb 27, 2006 14:50:40 GMT -5
London...my appologies...I got totally side tracked this week-end. I've been busy on the phone all morning and will be this afternoon...just wanted to let you know that and tell you that I do plan to follow up on this thread but haven't been able to.
Millenium, I haven't read Lizz's post but did read yours before posting my reply...I've had those mucous globs...more so now since adding a milkshake of DE to my nose. Under the scope...I really can't even make anything out. I see lots of red and blue fibers...clear ones too with specks. My gut feeling which has proved to serve me so well through out this disease is the it is a actual juvenile fluke newly released from the metacercaria...remember...they don't look like a worm with an actual body structure...they are very unusual creatures. I got a large one yesterday that I plan to stain and soak in iodine and look under the scope again but haven't had the time...I'll let you know what I find. The juvies don't have the same characteristic and identifiable features as the adult fluke...they are very difficult to identify as I have still yet to identify one.
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