selenocysteine tRNA
en.wikipedia.org/wiki/Selenocysteineleads me to this:
pyrrolysine inserted into proteins,,,,,,,,,,,,this is the charge?
UAG
Just throwing some things out, but, am seeing their way in.
Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.
Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA.
Department of Microbiology, 484 West 12th Avenue, The Ohio State University, Columbus, Ohio 43210, USA.
Pyrrolysine is the 22nd amino acid. An unresolved question has been how this atypical genetically encoded residue is inserted into proteins, because all previously described naturally occurring aminoacyl-tRNA synthetases are specific for one of the 20 universally distributed amino acids. Here we establish that synthetic L-pyrrolysine is attached as a free molecule to tRNA(CUA) by PylS, an archaeal class II aminoacyl-tRNA synthetase. PylS activates pyrrolysine with ATP and ligates pyrrolysine to tRNA(CUA) in vitro in reactions specific for pyrrolysine. The addition of pyrrolysine to Escherichia coli cells expressing pylT (encoding tRNA(CUA)) and pylS results in the translation of UAG in vivo as a sense codon. This is the first example from nature of direct aminoacylation of a tRNA with a non-canonical amino acid and shows that the genetic code of E. coli can be expanded to include UAG-directed pyrrolysine incorporation into proteins.
tinyurl.com/3mkzuspylT tRNA (CUA)
methanogenesomes:
Methanosarcina barkeri fusaro is a methanogenic Archaebacterium. Methanogens are organisms that make methane via a unique metabolic pathway with unique enzymes and cofactors. All of the methanogens are lithotrophs that can make their own energy only by methanogenesis. Most methanogens can make methane from carbon dioxide and hydrogen gas. Some methanogens can use other 1-carbon compounds, such as carbon monoxide or formic acid, and a few others can use acetate and methylamines. Enzymes in the metabolic pathway are very oxygen sensitive. Bacteria in the Methanosarcina family are slow developers and are sensitive to sudden change in physical and chemical conditions, but they are capable of growth in a variety of substrates. This metabolic diversity makes them an especially attractive target for genetic analysis.
The fusaro strain or M. barkeri was isolated from mud samples taken from the freshwater lake Lago del Fusaro near Naples (Italy). It has also been reported that Methanosarcina barkeri fusaro lives in the rumen of cattle. The rumen is an organ where microbial populations collaborate to digest cellulose and other polysaccharides, producing carbon dioxide, methane and organic acids. There is no oxygen in the rumen and these organisms are very oxygen sensitive. Therefore, Methanosarcina barkeri fusaro is an anaerobe, extreme anaerobe, if you will. The ecruate consists of methane, up to 35% and 65% carbon dioxide. Average daily production of gas from a cow is about 100-150 L a day. A well-fed dairy cow can generate as much as 500 L of gas daily. Methane gas from cows could be a major contributor to the problem with the ozone layer. -- Jessica Brill
genome.jgi-psf.org/finished_microbes/metba/metba.home.html used in L-pyrrolysine..........the charge?
the chemical reaction? .......... viral gastritis......................oh yeah.
The vapors?..........ha ha.........
It is methane that is making dead zones, from the sequestration of carbon by use of
buckyballs in the chemtrails. The excess carbon dumped into waters and those buckyballs
carry the pyrrolysine? makes more lipase?.......causes pancreatic problems?
causes skin problems.
It is the amber that causes the spark, should be in Alchemy buckyballs use fullerenes
carbon, nanotubes...........nanohorns, nanog time.
a microbe created to integrated into tRNA, is in agro bacterium.
working on it here, the dicty and the yeast.......
but the spark?
pyrrolysine........
next post, will try to do a follow through here.
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