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Post by chaosonline on Feb 25, 2009 20:12:59 GMT -5
OMG frito!!!!! Look what I found while I was surfing for your "lagenidium giganteum". Now where have we seen this before!!!! In depth info at the link below regarding the use of microbial pesticides (used mostly for mosquitoe eradication in Florida, California, Georgia and Texas) here: www.pubmedcentral.nih.gov/articlerender.fcgi?artid=528879Check out this insecticide produced as "Serenade" - This is the product that led me to this. www.serenadegarden.com/Karen
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Post by chaosonline on Feb 25, 2009 20:26:51 GMT -5
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Post by chaosonline on Feb 25, 2009 20:47:44 GMT -5
Molecular Genetics of Pathogenic Oomycetes (produces 10-kDa extracellular proteins, known as elicitins, which induce ... Green fluorescent protein) as noted in the thread titled "Robert F. Smith's research Thread and findings": tinyurl.com/crr5g5
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Post by bannanny on Feb 25, 2009 22:23:17 GMT -5
That cross section pic above reminds me of this hair for some reason... A few other things popped out at me too from the different fungi articles I was reading at your link... [glow=red,2,300]Bacillus thuringiensis suitable biological control agents[/glow].. isn't that the Bacillus that gets brought up alot here, other than the B. Subtilis? Or maybe was even identified in Robert Smith's study? [glow=red,2,300]Leptolegnia caudata was isolated from the malaria vector[/glow]... It's funny to me how malaria keeps coming up in things we've all been looking at lately. [glow=red,2,300]Only one species of the genus Lagenidium is known to be a facultative parasite of mosquito larvae, namely Lagenidium giganteum. The fungus has caused high mortalities in mosquito populations in many laboratories, small- and large-scale field studies (California and North Carolina.)[/glow]... I'd say the large scale studies were done in California (where I live.) We had quite alot of West Nile Virus cases here too tho last spring and summer. [glow=red,2,300]In spite of these non-target effects, a Lagenidium giganteum-based product was commercialized under the name Laginex by AgraQuest (California, USA) until 1999. It was claimed to be particularly effective against Culex spp., but the kind of spore used was not mentioned. The fungus is compatible with the bacterial agents Bti and Bacillus sphaericus Meyer and Neide when used against Culex quinquefasciatus, with the fungus having the distinct advantage over Bti in that it is able to recycle in stagnant water, infecting multiple and overlapping generations of mosquitoes. In field trials in which Laginex 25 was compared with Vectobac-12AS (Bti), Laginex reduced Culex quinquefasciatus larvae by 100% for 22 days whereas Vectobac-12AS required retreatment by the 10th day. Results from a small scale field trial in North Carolina indicated that Lagenidium giganteum recycled for an entire season despite periodic scarcity of hosts and short-term drought, with infections ranging from 0–100%. A large-scale field trial in Californian rice fields, using mycelium from either 20 or 30 liters of fermentation beer per hectare resulted in 40%–90% infection of Culex tarsalis and Anopheles freeborni sentinel larvae.[/glow]... infecting multiple and overlapping generations of mosquitoes eh? I think I keep getting overlapping infections too. Interesting that Lagenidium giganteum recycled for an entire season (what's in us can definitely recycle) and was claimed to be particularly effective... but the kind of spore used was not mentioned. Hmmmmm... makes me wanna know what that spore is? [glow=red,2,300]2.1 Coelomomyces The genus Coelomomyces consists of more than seventy species of obligatory parasitic aquatic fungi that undergo a complex life cycle involving alternating sexual (gametophytic) and asexual (sporophytic) generations. The genus has been found in all continents except Antarctica. Unlike most other entomogenous fungi, which have rather wide host ranges, involving species in several orders of insects, Coelomomyces is almost entirely restricted to aquatic Dipteran insects, including Culicidae, Psychodidae, Chironomidae, Simuliidae and Tabanidae. Susceptibility studies indicate that most Coelomomyces spp. are probably not host-specific, but nevertheless have relatively restricted host ranges[/glow]... the genus is found in all continents except Antarctica, eh? Exactly the same as morgellons. [glow=red,2,300]In reviews on Entomophthorales, their potential for biocontrol is often stressed because of high levels of infection observed in nature, and the theory that they can remain active in a site for several years. Release-experiments with Entomopthora maimaiga in Michigan showed that the fungus is able to establish itself easily in new territory and cause epizootics in target species. Unfortunately, no commercial formulations are yet available. For more detailed information about the biology, infection and host ranges of the order Entomophthorales, see Eilenberg (2002)[/glow]... maybe no commercial formulations but they did do release experiments and found it was able to establish itself easily in new territory and cause epizootics in target species. Plus, their potential for biocontrol is often stressed because of high levels of infection observed in nature, and the theory that they can remain active in a site for several years are morg sounding things to me. [glow=red,2,300]Trichomycetes are fungi that live hidden within the digestive tract of many arthropod species within several orders (including larvae of Diptera, Ephemeroptera and Plecoptera, and adults of some isopods, cladocerans, amphipods, copepods, Collembola, Coleoptera, and diplopods. They may be seen as branched or unbranched fungal bodies (thalli) firmly attached to the gut lining and lying within the gut lumen from which they obtain their nutrients. The class contains four orders, only one of which, Harpellales, contains a genus, Smittium, with mosquito-pathogenic species. For detailed information on the biology of Trichomycetes, see Lichtwardt (1986)[/glow]... Collembola and branching fungal bodies that firmly attach to the gut lining. Collembola have been a part of alot of morgies symptoms (including mine) and Dr. Hildy told me morgs is attached to the gut and if we don't get it out of there we're not gonna get over it at all. [glow=red,2,300]Other Anamorphic fungi that have been found on mosquitoes include some species of the genera Aspergillus, Fusarium, Paecilomyces, Penicilium, and Verticillium. Fusarium oxisporum Schlecht has been isolated a number of times from Ochlerotatus detritus (Haliday), and proved to be virulent to Culex pipiens in the laboratory, but later reports on the fungus are scarce. However, most of infections were either rare or the particular fungi were not considered highly pathogenic to mosquitoes[/glow]... Aspergillus and Fusarium, also mentioned in morgellons and also 2 of the different molds found in my house via a mold testing company. [glow=red,2,300]However, beyond gains in economic and public health terms, the stark reality of environmental impact and ever-developing resistance remains an issue of grave concern[/glow]... no sh*t sherlocks! That's a great page chaos. It really gives some good descriptions that are pretty much in laymens terms of different fungi. My mind was actually computing in a micro kind of way, but computing nonetheless. Anyway, those are just a couple of things that jumped out at me from all the fungi descriptions. Quite awhile ago I started putting whatever we find on a list in wordpad as being possible morg ingredients and try to widdle it down as we'd go. I lost it tho when my puter crashed and never started it up again. But I'm gonna start writing them all down again starting with your stuff here and whatever else I can remember that seemed to fit. Course it'll probably just remain a long list of things cuz they're all things I know nothin about! hugs ~~ bannanny
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Post by chaosonline on Feb 26, 2009 1:32:52 GMT -5
These altered biological control agents/parasites, used as insecticides, have the tobacco mosaic virus added to allow them to become a part of a plant and thus deliver the insecticide to the plant. Some have also added pheromones to attract the insects!!! Since the writing of this article a new ingredient is added, it is know as TMOF, which is a gene from the mosquito itself. This gene triggers a naturally response that inhibits the larva from digesting food, thus after a short time effectively killing the larva. This parasite is registered with the U.S. Environmental Protection Agency and several states, including California and Florida, for use as an operational mosquito control agent. It is currently the only commercially available biological control agent (not including Bacillus thuringiensis var. israelensis, which is a microbial insecticide) for mosquitoes. Besides being host specific, L. giganteum has the ability to recycle for weeks, months, or even years in a given breeding habitat after a single application. Lagenidium GiganteumLagenidium giganteum by James L. Kerwin, Mass Spectrometry Facility, Cornell University, Ithaca, NY 14853-2705 Lagenidium giganteum is a watermold that parasitizes the larval stage of mosquitoes. This microbial parasite belongs to a group of organisms that, although they look like fungi and have a "fungal lifestyle", nonetheless are related to diatoms and brown algae. The infective stage is a motile spore (see below) that goes on a search-and-destroy mission. The basis of its host specificity is selective recognition and attachment to its mosquito hosts. If a spore encounters, for instance, a water beetle, a dragon fly larva or a rice plant, it recognizes that a susceptible host has not been encountered. It will back off from that surface and swim on looking for a suitable host. Appearance Mosquito larvae (Culex tarsalis)) infected by the parasite. J.Kerwin, both L. giganteum is most easily recognized after it has matured, and ovoid, septate cells have formed. Infected larvae are recognized by a characteristic grey-white to almost completely white appearance. (Note that infection by some other parasites, e.g. microsporidians, will also result in pale white larvae). In the absence of competing bacteria or protozoa, infected larvae will be completely filled with cells that under a microscope will appear translucent. These cells are often most easily seen either in the larval head capsule or the anal papilla (the breathing appartus at the tail end of the larva). Habitat Although L. giganteum is not an obligate parasite, and can grow vegetatively (for example, on rotting vegetation or dead insects) in the absence of its hosts, it grows much faster and is easier to isolate from mosquito larvae. It can be found in freshwater habitats supporting mosquito populations. Isolation of the parasite from indigenous larval populations has been documented primarily in the southern United States, but it has also been found in California, Cuba, Colombia, and England. It will become dormant at temperatures below ca. 16°C or above 32°C. Moderate levels of salinity or organic load prevent it from sporulating, which is necessary for mosquito infection. Pests Attacked The parasite will infect and kill most species of mosquito breeding in fresh water, from temperatures of 16-32°C. It will also infect the closely related dipteran Chaoborus astictopus, the Clear Lake gnat, and at very high concentrations, some species of daphnids. Infection of daphnids is not desireable, but this only occurs when levels of the parasite ca. 100 times greater than that recommended for operational control are reached. Life Cycle Infection of a larval host is initiated by motile biflagellate zoospores that selectively recognize chemical signals on the epicuticle (outer exoskeleton) of mosquitoes. After attaching (A) the zoospores inject themselves into the larva, and ramify throughout the body of the host (B). Depending upon the temperature and zoospore density, the larva dies of starvation within 1-4 days. At that time each individual cell can form an exit tube and release 10-50 asexual spores, which in turn seek out a new host (C). Alternately, two cells can fuse (D), ultimately resulting in the formation of a thick-walled dormant oospore (E). This sexual stage of L. giganteum can remain viable in a dehydrated state for at least 7 years. It is this spore that is responsible for multi-year recycling of the parasite even though a habitat may be dry for months or years before reflooding and colonization by mosquito larvae. Under appropriate environmental conditions, oospores will germinate, resulting in the production of infective biflagellate zoospores similar to those produced during asexual reproduction. Relative Effectiveness Operational levels of mosquito control have been obtained by ground or aerial application of L. giganteum at rates ranging from ca. 0.9 x 1010 to 5 x 1010 CFU's (colony forming units) per hectare. The application rate depends upon the susceptibility and developmental rate of the target species, and habitat characteristics (temperature, organic load and salinity). For instance, control of floodwater Aedes species in early fall, in which there is synchronous hatch of large numbers of eggs in relatively cold water, would require treatment at the higher rates. At the other extreme, for 3- to 4-month control of very susceptible mosquito species such as Culex tarsalis breeding in rice fields at very low densities, the lower rates can be used. Zoospores do not have a cell wall, so are much too fragile to be applied directly in a breeding habitat; therefore, either presporangia (mycelia, USEPA registration No. 56984-2), oospores (USEPA registration No. 56984-3), or a mixture of both (USEPA registration No. 56984-1) are applied. Sporulation then occurs in the treated site to initiate infection. The sexual stage of L. giganteum has many advantages in an operational control program including multi-year stability, resistance to desiccation and abrasion, and it's inherent slow-release characteristics. Unfortunately, fermentation yields of oospores remain 2 orders of magnitude below that of the less stable mycelial (asexual, presporangial) stage. Research is continuing on improving oospore yields, which would be much more useful in large scale operational mosquito control programs. L. giganteum is a facultative parasite, and has been grown in large fermentation tanks using inexpensive culture media. Multi-hectare aerial applications have been made in rice fields and in refuges for migratory birds, primarily in California, where both efficacy and safety to nontarget organisms has been documented. Because the mycelial cells are relatively rugged, the low volume spraying equipment used by many mosquito abatement agencies for application of insecticides is readily adapted to application of this parasite. Pesticide Susceptibility Except for the dormant oospore stage, the parasite is very susceptible to organophosphates, carbamates, herbicides, fungicides, and other chemicals commonly used either by mosquito abatement districts, or applied to crops (such as rice and soybeans) that are associated with mosquito breeding. IC50 values for inhibition of mycelial growth commonly range from ca. 50-5,000 ppm. Fortunately, recommended application rates for many of these chemicals are below levels that are toxic to the parasite. Organic solvents such as toluene and oil commonly used as diluents of the active ingredients of pesticides are also toxic to L. giganteum. Commercial Availability This parasite is registered with the U.S. Environmental Protection Agency and several states, including California and Florida, for use as an operational mosquito control agent. It is currently the only commercially available biological control agent (not including Bacillus thuringiensis var. israelensis, which is a microbial insecticide) for mosquitoes. Besides being host specific, L. giganteum has the ability to recycle for weeks, months, or even years in a given breeding habitat after a single application. Beginning in 1997 the asexual stage of this parasite will be commercially available as the LAGINEX® formulation. It can be obtained from AgraQuest, Inc. www.nysaes.cornell.edu/ent/biocontrol/pathogens/lagenidium_giganteum.html_______________________________________________ There are a few pictures if you take the link to view the article. Karen
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Post by fritolay66 on Feb 26, 2009 8:12:33 GMT -5
Hi Karen, I just spent an hour going through all this, inserting quotes, adding my interpretations, and went to preview, and then went back to look at the messages so I could add some more, and poof, everything is gone! How disheartening, as I have been working doubles since monday and have three more double days to go. %$@*!! This is awesome research you have done. Bananany, I also was doing your message too. I am sorry ladies, I just can't repost my ideas at this time. I know this is one part of the equation. I want to know if exposure risk has been determined for humans and animals in the environment. As both seem to be experiencing fungal infections and increases in zoonotic disease. I would also like to know HOW they are getting this into the environment. I think there is more than one method they are utilizing. I am also interested in what the mosquito larval coat is made of and the chemical reactions there of and if there are any similarities to us, such as the portals in which we could be exposed. Lungs, sinuses, GI, and skin mainly. There is one quote I'd do have to mention now. I interpret this as admission as to what our environments have been repeatedly sprayed with, or if you go with the first links reference, infected with. What predatory bacteria, protozoa, and nematodes. How would this combo affect the use of oomycytes. Could these also become target organisms since their numbers would effectively reach a 100 fold increase and there for be parasitized by L. gig. due to use as an natural insecticide. Absolutely awesome posts! Thanks for following up when I couldn't. I am very excited to see what you two find out. Frito
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Post by chaosonline on Feb 26, 2009 14:35:44 GMT -5
When I titled this thread I was referring to the pictures on Cliff Carnicom's site. If you take the time to browse you will see that several pages there have photos of specimens that are very similar to the one above. Here is what he says about the similar fibers: "The finding here is that there is essentially identical form, size and structure between the airborne filament samples that have been reported on extensively over the years in connection with the aerosol operations, the morphology of at least one characteristic Morgellon's fiber and with a series of blood anomalies that have recently been documented. There are now major considerations before us because of this." www.carnicom.com/contrails.htmwww.carnicom.com/contrails.htmfrito this is being sprayed on crops in a variety of ways including ariel spraying. Here is a link to product inforamtion: www.pesticideinfo.org/Detail_Chemical.jsp?Rec_Id=PC38305Ok so now I see the entire story of "lagenidium giganteum" is revealed in the Kaiser Papers which I had heard about but never read. This even mentions AgraQuest, Inc. the company that makes Serenad. Here is a link but you must scroll to the side to read: biotechnology.kaiserpapers.info/Here is a link to a site about "Serenade" called "Voices of the People @ FORUM.STOPTHESPRAY.ORG • : forum.stopthespray.org/viewtopic.php?f=5&t=2209Karen
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Post by chaosonline on Feb 26, 2009 15:21:06 GMT -5
Here is a pertinent quote from the Kaiser Papers website: THE WORKPLACE INJURY/ILLNESS/DISEASE of David Bell and others, WAS NEVER REPORTED BY AGRAQUEST AS REQUIRED BY FIFRA 6From 1983 to 1990, Pam Marrone was in charge of the Insect Control group at Monsanto Agricultural Company. Her group was instrumental in pioneering projects in genetically engineered microbial pesticides and transgenic crops for insect control. After Monsanto, Pam was founding president of Entotech, Inc. Later, Pam left to start up a new company called AgraQuest, Inc. One of the life forms that Marrone and her crew were genetically modifying is Lagenidium giganteum. Around the time that this life form was being altered, a new disease emerged that has been named Lagenidiosis. Prior to this manipulation of the genetic structure of Lagenidium giganteum it was unheard of or "unrecognized" for it to cause illness in humans. At the same time no one known had ever mixed in a genetically modified brew of "Swamp Cancer" -Pythiosis with Lagenidium giganteum before either. See: medtech.cls.msu.edu/medtech/mendoza/ The following is some background information on lagenidium giganteum. Please note that some of this is contrary to outdated EPA literature so you can understand why while it may sound as if it is innoculous, some people believe that it can be very dangerous: Lagenidium giganteum is an Oomycete fungus which is pathogenic to a number of mosquito species, including Aedes, Culex, Anopheles and Culiseta (Tanada and Kaya 1993). Lagenidium giganteum is a parasitic, yeast-like water mold or fungus. The life cycle begins with a motile zoospore (asexual stage) that seeks out mosquito larvae, attaching to and penetrating the cuticle via a germ tube. The fungus grows inward, eventually filling the body cavity and killing the mosquito larva. The fungus can then be released from the infected cadaver, generating more zoospores that can infect other larvae. The sexual cycle produces oospores that can maintain the fungus during unfavorable conditions, such as long periods of drought. Upon flooding, oospores release infective zoospores to start the cycle again. 1.3 Lagenidium. Only one species of the genus Lagenidium is known to be a facultative parasite of mosquito larvae, namely Lagenidium giganteum. It consists of two stages: oospores (sexual), and zoospores (asexual) (See Fig. 1). Although this fungus has been named Lagenidium culicidum Umphlett in some publications (Umphlett and Huang 1970; McCray et al. 1973), this was later shown to be Lagenidium giganteum (Couch and Romney 1973).] biotechnology.kaiserpapers.info/Also, don't miss this video:
The following short video shows some of the material that has infected these microbiologists that are being denied WC coverage. This material came out of one of the AgraQuest employees that was exposed to their products. Click the following to view the film clip:biotechnology.kaiserpapers.info/media/P8080325.MOVKaren
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Post by bannanny on Feb 26, 2009 18:26:05 GMT -5
Is there any way to resize this page so it isn't so wide? It's makin it hard for me to read... got alot of fog today is probably why. But it sure would help if someone could fix it... I would but I don't know how to, duh. I'll have to come back to it later. Just want to say I know how frustrating that is when you write a bunch of stuff down and it disappears before you can post it... very irritating eh? Sorry it happened frito.
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Post by chaosonline on Feb 26, 2009 18:55:16 GMT -5
Bannanny I think I fixed the problem by resizing the picture in my first post. Let me know if that did the trick.
Karen
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Post by bannanny on Feb 26, 2009 19:28:14 GMT -5
You did it!! Thanks for that chaos... I can read over it so much easier now. You're a real gem you are!
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Post by bannanny on Feb 26, 2009 19:57:27 GMT -5
Wow, that video link messed my puter up... it's been running strange today tho. I ended up having to close it out with Task Mgr. and then I couldn't even get Task Mgr. to close! Weird...anyway, needless to say I couldn't watch it.
Sorry about you losing everything you put together frito... that's happened to me before too and it sucks!
Here's what I picked up this time from the info...
[glow=red,2,300]L. giganteum has the ability to recycle for weeks, months, or even years in a given breeding habitat after a single application. The infective stage is a motile spore (see below) that goes on a search-and-destroy mission. [/glow]
To me that goes right along with morgs, but...
[glow=red,2,300]If a spore encounters, for instance, a water beetle, a dragon fly larva or a rice plant, it recognizes that a susceptible host has not been encountered. It will back off from that surface and swim on looking for a suitable host. It will become dormant at temperatures below ca. 16°C or above 32°C. [/glow]
This says it's host specific so how could it infect us... unless maybe it's cuz we've been bitten by an already infected mosquito?
Also... in farenheit terms, where would 16 and 32 degrees celcius fall, do you know? The reason being I know morgs doesn't become dormant in weather that's below 32 degrees farenheit cuz it's been colder than that here and it's still active in me.
[glow=red,2,300]Organic solvents such as toluene and oil commonly used as diluents of the active ingredients of pesticides are also toxic to L. giganteum.[/glow]
Morgies test results from Dr. Hildy's blood tests always show we have toluene in our blood... mine did as well. So being toluene is toxic to L. giganteum, our blood would kill it right?
That's what I'm picking up anyhow.
hugs ~~ bannanny
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Post by bannanny on Feb 26, 2009 20:02:52 GMT -5
Oooops, forgot to ask you somethin.
Is the L. giganteum what we're looking at, or is it the spores it gives off or what? Sorry, but I'm all confused again today.
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