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Post by skytroll on Jan 11, 2008 3:48:29 GMT -5
Remember Grady,
The theory of Evolution has not been proven, I do not think the area of the flagella has reached a conclusion yet. Only inference, no proof only the novel organisms that fit the intermediary stages.
Anyone can play the New Tree of Life, has all the intermediaries in there, all the new novel ones, these were not discovered they were created.
So, your theory is just as valid as the Evo Devo's! If yours isn't theirs isn't either.
Well, deep into the labs we shall wonder, and when the rat can talk, boy o boy will there be a front page article in THE NEW YORK TIMES!
But, a disease eating away at the land and the people, NOT EVEN IN THE PAPER!
And who will ride in on the BIG GOLDEN HORSE?
Hang in there people, we will keep working on this theory because I don't think we have all the evidence yet.
Skytroll
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Post by robertalouise26 on Jan 11, 2008 6:12:16 GMT -5
Dearest Betsy can you tell me what is going on? What have I missed when my computer was down. I remember Grady saying that he was leavng the boad THAT IS A GREAT GREAT SHAME HE HAS SOOO MUCH TO OFFER. wHAT HAS HAPPENED TO YOU BETSY ARE YOU THINKING OF LEAVING THE BOARD AS OLL? iT WILL BE OUR LOSE AND YUOR LOSE. I am sure that this can be worked out with out such drastic happenings. You also have so much to offer. I do hope to hear from you Betsy. Much love and hugs.Roberta
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Post by godog on Jan 11, 2008 10:33:15 GMT -5
I would like to download this thread, but I don't know how. I'm on my way to work, but would like to download this sometime if someone could tell me how. Prolly easy, but not easy for me.
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Post by jeff on Jan 11, 2008 10:43:07 GMT -5
I wonder if they had this bacteria back when the exxon valdez sank ? Because I am a surfer and I frequent the waters off the pacific northwest coast..with the currents the residue would end up here from the alaska current.
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Post by ANTHILL on Jan 11, 2008 11:39:09 GMT -5
I would like to download this thread, but I don't know how. I'm on my way to work, but would like to download this sometime if someone could tell me how. Prolly easy, but not easy for me. godog You can copy and paste each page of this thread to your word pad or other word processor software of your choice on your computer or much easier click on "send this topic to a friend" button that is by the reply button and send the link to yourself and save the link in a file in your email box
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Post by skytroll on Jan 11, 2008 13:44:55 GMT -5
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Post by skytroll on Jan 11, 2008 14:17:35 GMT -5
CbrA CbrB a connection to B. Subtilis which I believe can form the spores in the air? Seems B. subtilis, a gram positive forms with this maybe? So the mover stuff. something moves the plastic besides our heat of our bodies? just thinking here. "Nutritionally versatile Pseudomonads effectively utilize various nitrogenated compounds, including amino acids (such as glutamate, proline, arginine and histidine) as sources of carbon, nitrogen and energy. The histidine catabolic pathway of Pseudomonas was first established in Pseudomonas fluorescens followed by Pseudomonas putida and Pseudomonas aeruginosa. The genes for the pathway enzymes were then identified and characterized in P. putida. Expression of the P. aeruginosa and P. putida histidine catabolic enzymes absolutely requires the presence of histidine (or its degradation intermediate urocanate) and is subject to regulation by both carbon and nitrogen, as in Klebsiella aerogenes (formerly Aeromonas aerogenes) and Bacillus subtilis (for reviews, see refs [9,24,57,85]). www.springerlink.com/content/k7j5phj63rg7m8g4/skytroll
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Post by skytroll on Jan 11, 2008 14:20:38 GMT -5
Abstract A wide variety of proteins are exported or secreted by a range of morphologically distinct bacteria. The processes of protein export are most extensively characterised in Escherichia coli, where recent advances have been made in the identification of genes involved in forming the export machinery. Both Gram-positive and Gram-negative bacteria secrete proteins into the medium. Gram-negative bacteria have adopted a variety of approaches in order to overcome the additional permeability of the outer membrane. E coli seems to always be involved in this, remember first model in the Evolutionary Project and the Genome Project. www.blackwell-synergy.com/doi/abs/10.1111/j.1574-6968.1985.tb01180.xskytroll
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Post by skytroll on Jan 11, 2008 14:26:22 GMT -5
BIOFUEL GASES from landfills? It is in the gas! he capturing and use of landfill gases is also mentioned, as well as microbial coal liquefaction. Described is also the construction and performance of microbial fuel cells for the direct high-efficiency conversion of chemical fuel energy to electricity. Bacterial carbon dioxide recovery is briefly dealt with as an environmental issue associated with the use of fossil energy. If these p. putida constructions are used in landfills and landfills are using the gases toulene is in the landfills and plastics. so on and so forth. and the use of algae fuel. WHERE IN THE HELL IS THE FREE ENERGY? We do not need these blasted biofuels? DOE you have screwed up big time and took the Agriculture right along with it! Emphasis is also put on microalgal lipids as diesel substitute and biogas/methane as a renewable alternative to natural gas. microcystis aeruginosa and putida in the gas? Forming in air with the gas, B. Subtilis helps make the spores! Toulene gas, algae gas, landfill gas METHANE! What a mess! www.blackwell-synergy.com/doi/abs/10.1111/j.1574-6976.1995.tb00161.xSkytroll
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Post by skytroll on Jan 11, 2008 14:32:00 GMT -5
Adhesives: Bacterial Polymers: Physicochemical Aspects of Their Interactions at Interfaces Thomas R. Neu School of Microbiology University of New South Wales P.O. Box 1 Kensington, N.S.W. 2033, Australia Kevin C. Marshall School of Microbiology University of New South Wales P.O. Box 1 Kensington, N.S.W. 2033, Australia How do bacteria stick to a surface? There is still not enough information about to answer this question especially at the molecular level. This question only gives rise to more questions. What is the structure of the true adhesive bacterial polymer? Is only one bacterial polymer or several polymers involved in the adhesion process? What is the role of proteins associated with the bacterial polysaccharides? What type of polymer is produced for the adhesion to hydrophobic surfaces? Is the polymer produced as a response to the surface ? This review is an attempt to summarize the physicochemical aspects of bacterial polymers and their interaction with surfaces. It was tried to give an overview of the literature published in this field. The article is divided into the following sections: first, the forces involved in bacterial adhesion are described. Second, the theoretical approaches to bacterial adhesion are discussed. Third, different fluid conditions are investigated. Fourth, the nature of different bacterial polymers which are important for the interaction with a surface is elaborated. Fifth, the current knowledge about biological polymers at interfaces is shown. And sixth, the role of polymers in the adhesion of bacteria available to date is highlighted. jba.sagepub.com/cgi/content/abstract/5/2/107skytroll
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Post by skytroll on Jan 11, 2008 14:49:27 GMT -5
Expression of the TOL plasmid xylS gene in Pseudomonas putida occurs from a alpha 70-dependent promoter or from alpha 70- and alpha 54-dependent tandem promoters according to the compound used for growth. Growth of Pseudomonas putida (pWWO) on alkylbenzoates requires the expression of the meta pathway operon, which is mediated by the XylS protein after binding of a benzoate effector. Alternatively, in cells growing on toluene or its aromatic alcohols, overexpression of xylS mediated by XylR activated by these compounds leads to overproduction of the XylS regulator, which even in the absence of benzoate effectors stimulates transcription from the meta cleavage pathway operon promoter. We show here that in bacteria growing on glycerol or alkylbenzoates, the xylS gene is expressed at a low but constitutive level from a newly found sigma 70-dependent promoter called Ps2. The amount of XylS protein made from the transcript originated from Ps2 was sufficient to allow high levels of expression from the meta cleavage pathway operon promoter when the cells were grown in the presence of 3-methylbenzoate. The transcription initiation point of the transcript generated from Ps2 mapped 9 bp upstream from the proposed ATG of the xylS gene; this transcript contains the ribosome-binding site. The Ps2 promoter was located 110 bp downstream from a previously described sigma54-dependent promoter located upstream from the xylS open reading frame, now called Ps1. In cells growing on toluene or benzyl alcohols, the XylS regulator is overproduced as a consequence of increased expression of the gene through the effect of the two promoters working in tandem: the newly found sigma 70-dependent promoter, whose expression is XylR and toluene independent, and the sigma 54-dependent promoter, whose expression is dependent on XylR activated by its effectors. This expression pathway of the xylS gene explains why sigma 54-deficient P. putida bearing the wild-type TOL plasmid, or the wild-type P. putida strain bearing a TOL plasmid with a knocked-out xylR gene, can grow on alkylbenzoates. Until now this has been one of the unresolved paradoxes in the transcriptional control of the TOL meta cleavage pathway. www.pubmedcentral.nih.gov/articlerender.fcgi?artid=177945jThis gets deeper and deeper! Skytroll
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Post by skytroll on Jan 11, 2008 14:57:29 GMT -5
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Post by godog on Jan 11, 2008 17:49:30 GMT -5
Thanks Ant, I never thunk of that one. I've emailed myself for other stuff, but I hadn't thought of it yet for this. I'm too tired to think hard.I walked hard today. Haha.
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Post by skytroll on Jan 11, 2008 18:33:21 GMT -5
filaments associated with p. aergunisa. "he helical filaments of the bacterial flagella so far studied seem to be universal in the bacterial kingdom. Despite the variation in flagellin molecular masses, which range from 24 kDa to 62 kDa in different species, there are only two forms: either the so-called Normal (left-handed) or the Curly (right-handed). The Normal and Curly helical forms are asymmetric; the two characteristic helical parameters, which are the pitch and diameter, of Normal filaments are twice those of Curly filaments. Both the universality of these two helical forms and their asymmetry are biological puzzles. We found that the marine bacteria Idiomarina loihiensis have flagella with left-handed Curly-like filaments. Analysis of the polymorphic forms under different pH conditions showed that the Curly-like filaments are actually Normal filaments having a smaller pitch and diameter than those of Salmonella typhimurium. A minor modification of Calladine's model for a filament lattice can explain the variant helical forms. Pseudomonas aeruginosa filaments also belong to the family of I.loihiensis filaments. Thus, there are at least two families of flagella filaments." p aeruginosa belong to I. loihiensis filaments family Skytroll tinyurl.com/2l3lz2www.ncbi.nlm.nih.gov/pubmed/16120444?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_ RVAbstractPlus skytroll
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Post by skytroll on Jan 11, 2008 18:39:42 GMT -5
What we need to deterimine is was this constructed or did it evolve from a natural organism.
If engineered, generally those are better controlled.
If put in environment as a way to change DNA in the environment, is another thing.
I think we are looking at bottom up approaches where there is not control, only adaptation, and that means humans.
We are looking at something that can be genetically engineered, where there is not control in the transgenic application. The reactions of the organism to the chemical causes another reaction to the original organism which then can effect the host in a way that is not predictable, depending on the makeup of the host's DNA.
When Homeobox proteins are now infiltrated into the environment, and human proteins are put in environmental microbes, did they ever consider that quorem sensing would kick in and whatever organism in environment that has human proteins in it will be attracted by aeromonads, or pheromones of the host. The organism will follow the aroma.
Skytroll
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Post by teller on Jan 11, 2008 20:14:59 GMT -5
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Post by skytroll on Jan 11, 2008 20:48:46 GMT -5
Teller, interesting what that mussels were caged and let loose, these mussels are everywhere....... and I am sure they are secreting the proteins that carry the toulene and I wonder what kind of fertilizer was used in the cleanup of the oil. * total hexane-extractable hydrocarbons in the water column * nitrogen and phosphorous nutrients * plankton chlorophyll * total aromatic hydrocarbons bioaccumulated in mussels (held in cages at the low tide zone of the fertilized shorelines) * water sample toxicity using a standard effluent toxicity test program. (This test is designed to detect any general toxicity associated with the nutrient addition operation.) ,,,,,,,,,,,,,,,, aromatic hydrocarbons bioaccumulated in mussels? well then.........and released? /////////////////' Toxic Effects of Unresolved Complex Mixtures of Aromatic Hydrocarbons Accumulated by Mussels, Mytilus edulis, from Contaminated Field Sites "Exposure of marine mussels (Mytilus edulis) to an unresolved complex mixture (UCM) of aromatic hydrocarbons isolated from a crude oil has been shown to reduce their feeding rate by 40%. The present study was undertaken to determine whether UCMs bioaccumulated by mussels in the field are also toxic. The feeding rate of mussels derived from polluted sites increased when they were placed in clean water, pointing to a loss of toxic agents from the tissues. At the end of the depuration period, water in which mussels from an oil-polluted site had been held contained a UCM. Steam-distillation extracts of the tissues of mussels taken from several polluted sites were shown to be highly toxic to the feeding activity of juvenile mussels. The tissues of mussels from these sites contained UCMs. Nontoxic steam distillates from clean mussels did not. Steam-distillation extracts of mussels from an oil-polluted site were fractionated by normal-phase high-performance liquid chromatography. A fraction, largely comprising a "monoaromatic" UCM, reduced the feeding rate of juvenile mussels by 70%. Two later-eluting fractions containing aromatic UCMs also produced smaller depressions in feeding rate. These results support our contention that some aromatic UCM hydrocarbons constitute a forgotten pollutant burden in the marine environment."... pubs.acs.org/cgi-bin/abstract.cgi/esthag/2003/37/i21/abs/es021053e.htmlUCM hydrocarbons? Abstract Mussels (Brachidontes Sp.) collected in April 2000 from the Red Sea coastal of Egypt in order to determine the contaminationby hydrocarbons. The study included 11 sampling stations covering about 450 Km of the Egyptian Red Sea coast for the first time. Total concentrations of 20 aliphatics (average 0.105 ng g-1 of wet weight) and sixteen polycyclic aromatic hydrocarbons (PAHs) (average 5452 ng g-1 of wet weight) were generally lower in comparison with those reported for some of the published surveillance and monitoring studies of coastal areas from various regions. These results suggest that the coastal area in the Red Sea of Egypt mightbe considered as a relatively unpolluted. aliphatic - Brachidontes sp. - chemical pollution - Egypt - hydrocarbons - mussels - PAHs - pollution monitoring - Red Sea www.springerlink.com/content/n5v2133443862755/WELL THEN THE PAHS? the Unresolved complex Mixtures? polycyclic aromatic hydrocarbons: CDC's TAKE ON PAHS: www.atsdr.cdc.gov/tfacts69.htmlOf course it will be blamed on pollution, NOT the mistake made in the aromatic hydrocarbons just what are those? Skytroll
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Post by suebe on Jan 11, 2008 20:57:57 GMT -5
Grady please don't stop posting on this thread, I think we should keep it going and going just like the energizer bunny.
Look I've begged the CDC to come to my home, come get me, you KILLED my child, Mr.Dill, Travis, Trevor, Karen, need we go on.
I want to lick Dan Rutz and Dave Daigle right on thee face. When I was in Jr. High my boyfriend was a Dave Daigle and when I heard the name I freaked, but I'd still like to kiss HIM and put his a$$ down with this horror if he is now working with CDC.
Grady even if one of the great doctors working with us, scientists, anyone trying to help. If they find the TRUE answer, CDC will do nothing more then work to discredit them, just like they have done to the Lyme Docs.
They think we are nothing, even if 100,000 of us dropped dead from this tomorrow they would not break a sweat.
Because they know what it is, I'd swear they know. Right now they are just working on the cover up.
Don't you dare let them or anyone scare you off.
OHH AS BERNIE MAC SAYS
I WISH THEY WOULD I"LL PAY THE AIR FARE FOR THEM TO GET TO MY A&&
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Post by skytroll on Jan 11, 2008 20:58:23 GMT -5
Wonder when phytoremediation came about? "Microorganisms, especially bacteria, are the primary transforming agents for these toxic compounds in natural environments such as soils and sediments. An understanding of bacterial population structure and genetic interactions is necessary for the successful application of in situ bioremediation technology, whether based on indigenous microorganisms or introduced genetically-modified microorganisms. Detailed genetic analysis has taken place for several PAH-degrading and metal-transforming bacterial systems, especially plasmid-encoded determinants. Little is known, however, about the survival, function, and transfer of these genes in natural environments. This is especially true in contaminated Superfund soils and sediments, which are typically complex systems containing more than one toxic organic chemical or heavy metal. Project 6 will act as an interface between basic genetic, biochemical, and physiological studies of degradative microorganisms and the application of these studies, specifically the bioremediation of contaminated soils. These studies will use many of the genetic tools developed by this program and by other researchers to support the application of bioremediation in biofilms and in phytoremediation using the plant rhizosphere. "..... more on PAHS here: www.med.uc.edu/sbrp/researchcores.htmlCould this be where the Ti-Plasmid of Agrobacterium comes in? To fix the PAH problem or is that what started another type of PAH problem? Rhizosphere is in agro ti plasmid. Plasmid means something> Plasmid: "A plasmid is an extrachromosomal DNA molecule separate from the chromosomal DNA and capable of autonomous replication. " skytroll
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Post by robertalouise26 on Jan 11, 2008 21:01:25 GMT -5
Hi skytroll a few weeks ago Sidney and I were talking about this sticky glue that appears on my dogs nose and also on my skin. Sidney had read somewhere about the glue that is in mussels. Maybe yu should ask her about it Love and best wishes. Roberta
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