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Post by skytroll on Jan 11, 2008 21:09:48 GMT -5
Method 1664: N-Hexane Extractable Material (HEM) and Silica Gel Treated N-Hexane Extractable Material (SGT-HEM) by Extraction and Gravimetry (Oil and Grease and Total Petroleum Hydrocarbons) EPA Number: 821B94004b Date of Publication: April, 1995 Pages: 27 Audience concerned citizens, industry Publication Comments Available from: NTIS # PB95-239232 Ordering Information REALMS # D084 Ordering Information [BE SURE TO INCLUDE THE ERRATA SHEET FOR EQUATION 1] Method 1664 was developed by the United States Environmental Protection Agency Office of Science and Technology to replace previously used gravimetric procedures that employed Freon-113, a Class I CFC, as the extraction solvent for the determination of oil and grease and petroleum hydrocarbons. More... Keywords: Water Pollution Monitoring Hexanes Extraction Gravimetric Analysis Test Methods Chemical Analysis Silica Gel Quality Control Pollution Regulations Oils Greases Crude Oil Petroleum Products EPA Method 1664 tinyurl.com/35vywryosemite.epa.gov/water/owrccatalog.nsf/852887bbc1ca359585256ad400705867/205e37050954ecba85256b0600723f74!OpenDocument Skytroll
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Post by toni on Jan 11, 2008 21:11:53 GMT -5
Skytroll,
The link you posted to Robertalouise, about the insects infected by horizontal gene transfer in NZ...is it too long to post that article? I'm not able to see it when I click that on, and I'm most terribly interested. Thank you!
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Post by skytroll on Jan 11, 2008 21:24:36 GMT -5
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Post by skytroll on Jan 11, 2008 21:45:33 GMT -5
we have tons of mussels here and we have mycocystis aeruginosa in the small lakes, not in rivers but in still water or small lakes and of course the Great Lakes. They do have a glue like substance but they also were used to clean up oil spills so this could have brought all of this into those waters. Ocean carriers, will have these on their hulls. It is so related to water, and with the B. subtilis forming the fungus in the air, But the adhesive can come from the mussels. Mussel images, have you seen many of these? www.boat-ed.com/ky/course/p4-13_wastedischarge.htmNow I am thinking why the plankton is gone, these zebra mussels eat them? and they bring in all kinds of other species of bacteria, and they could be bringing in the p. putida zebra mussel shells: www.uwgb.edu/BIODIVERSITY/econotes/2001/pow20011209large.htmmore images; Now if they carry the toulene or whatever they aborb they will carry the bacteria, fungi or the pseudomonad right along with it. tinyurl.com/27zo79www.altavista.com/image/results?itag=ody&mik=photo&mik=graphic&mip=all&mis=all&miwxh=all&q=zebra+mussels&stq=20 skytroll
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Post by toni on Jan 12, 2008 9:32:57 GMT -5
Sky, I don't mean to be a pain, I don't know how to "access" that file once it's downloaded. Thank you.
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Post by toni on Jan 12, 2008 9:55:48 GMT -5
I did find this though. www.esr.cri.nz/competencies/populationhealth/genetransfer.htmAnd you know what just astounds me to pieces, is that "science" is talking out of both sides of their mouths when it comes to horizontal gene transfer. This is extremely important to us I do believe, actually all living things. The "excitement" science has about (delivery of drugs into the human body) by horizontal gene transfer is like to them finding (the new world) ....yet the flip side of the coin is how gmo can possibly be changing or adding to our bodies inability to use ingested medications because of HGT already. I can't understand why HGT hasn't been top of the list in what IS happening already because of the transgenic crops etc - it's as though they're only looking at one side of the coin. Do you know what I mean? They're actually "admitting" this is a way to "deliver drugs into the body more efficiently, and how much lateral and horizontal gene transfer IS capable, and there's great possibilty gmo can cause problems with drugs ingested because of gmo.....yet hahah they're not even talking about "what is happening presently" by ingesting gmo and DNA. Isn't that unreal...that to me is the twilight zone! Like they're saying okay folks who all eat GMO....we're not going to discuss "what's happening right now"....but we want to discuss what we can (do) in the near future with GMO because we KNOW it's got the capacity to "intermingle" with existing DNA.
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Post by toni on Jan 12, 2008 10:03:36 GMT -5
In fact look at this: September 4, 2007 Transmission electron micrograph of Wolbachia within an insect cell. Image: Scott O'Neill.Wolbachia has on some occasions inserted almost its entire genome into species that it infects, reported scientists at the J. Craig Venter Institute and the University of Rochester. (( This is the first example of large-scale horizontal gene transfer between single-celled and multicellular organisms. Although horizontal gene transmission is common among single-celled organisms, it is rare among multicellular organisms, and large scale transfer like that of an entire genome had previously not been suspected.)) I love it, they'd "not suspected" this event to occur....talk about the horses out after the doors been wide open. tinyurl.com/yvcz23
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Post by toni on Jan 12, 2008 10:14:39 GMT -5
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Post by skytroll on Jan 12, 2008 10:26:47 GMT -5
But, this is what they planned, though.
For prokaryote single cell to multi-celled is the key in Evolution.
For evolution to take place, the one celled had to be incorporated into the multicelled which is human, insects, animal, plants.
This is what Darwin meant by intermediary, There was none, but directed or forced evolution will make something die to take it's place.
What they have done is gone into the proteins and the cells themselves. And now on molecular level.
Wolbachia is the bacteria in Onchocerciasis, the worm disease.
It is in the gut of the termite, I believe and most likely in many insects, as in us.
This is where the one-celled has become part of the multicelled.
So, this then gives Venter the opening to make artificial genes. To create them for humans.
Oligonucleotides.
You see it all stems from the evolutionary theory that if it is not there, we shall create it.
On the other hand, the ag people are using the protein transfer techniques, and they call it an "Art" Those who know the art.
Transgenic, promoters, receptors, transfers, and where they fall down is the flagellum, the mover, where did that come from?
But, once we see where this began, then one can see why the foods have the proteins altered in them, so we are eating different protein construction from what we were before.
It is rare among muticellular organisms, because it has never happened before, this is where they crossed the line.
skytroll
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Post by janedough on Jan 12, 2008 10:58:26 GMT -5
REMEMBER THIS FROM BIOLOGY ONLINE? ? Sky, your last post made me want to revist the old board. : by Gaiapacha on Wed Oct 05, 2005 5:48 pm The fiber disease constitutes an infection with a genetically modified quorum sensing stem cell related micro organism. Basically the organism represents a cyano bacterium that knows a stage as a giant cell and as a mold. Diversification resulting in morphologically different acting micro organisms takes place by shaft, giant cell but also by fission. Hence a coccidiod cyano bacterium like(single, diploid and chain forming)micro organism that lives on the skin and in wounds is the mayor responsible agent. No association is made by micro biologist or histopathologist in connection to this pathogen. Nota bene, Intermediate stages resemble a (quorum sensing) transparant gel that constitutes a protoplasm. This makes detection of the agent in biopsy impossible during standard clinical research. Single cell micro organisms function on a multi cellular level, communication between species of the same kind as well as different kind is possible. The micro organism has been fused with a mayor parasitic protozoa, a butterfly(lepidoptera) and a mammal specie. Multi lineage differentiation is fact. The fibers constitute protein. Specks are chitin like polymers. Fibers represent cell that are most connected to sensing parts like antennea, tongue, feet and wing. The colors of the fibers are connected to the colored wing pattern of lepidoptera. Actually they constitute variants with a semi parasitic nature. Keratin erosion is evident. The breeding ground for the organism in skin is almost exclusively the follicle(stem cell per definition) The phenomenon is directly linked to proteome and genome research and it concerns commensal bacteria without the status of known pathogen, but some members of the tree are known as nosocomial (like the pseudomona) Infection resembles most protothecosis. Amphoteracine B and amikacine will be the most logic choice for therapy but no protocol exist for this type condition. Sulfa Methoxazole/ Trimethoprim and Tetracycline and derivatives are suppressive but not curative. Also azoles like itraconazole, econazole, micanozole are effective. Infection may directly or indirectly relate to IBS, fibromyalgia, chronic fatigue syndrome, therapy resistant skin lesions, onycholisis, neurogenic bladder and chorioretinitis. Sincerely, GaiapachaGaiapacha Garter Posts: 1 Joined: Wed Oct 05, 2005 3:10 pm
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Post by toni on Jan 12, 2008 11:21:14 GMT -5
Great post, thanks for finding that.
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Post by felixwillford on Jan 12, 2008 11:48:40 GMT -5
Sue, my best friend Sheila too. Because Doctors didn't know what to do.
Shiela Died on her 46th birhday. New Years Eve 2004.
They never suspected Lyme and never recieved treatment that would have allowed her to be here today to see her daughter get married.
She believed in the Doctors and the CDC did not INFORM doctors how prevealant Lyme and all these modified disease were. So I can't blame the Doctor.
I blame the CDC for taking the best friend I ever had off this earth.
She loved life.
She did alot of gardening an planting flowers and I know much time in the soil.
She filled Vending machines at Alcoa in Newburgh Indiana where Aluminin is processed and puttng contaminants in our air.
My dear friend is missed by family and friends.
THE CDC is the most irresponsible and apparently ignorant to what they are suppose to be doing.
Alerting and working with Doctors so people can get proper treatment.
People need to be fired from the CDC and have thier medical Licenses taken away.
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Post by suebe on Jan 12, 2008 12:32:06 GMT -5
Kmarie AMEN SWEETIE
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Post by Sidney on Jan 12, 2008 14:38:24 GMT -5
Cutaneous or sub-cutaneous bacterial infection or fungal infection can create a glue-like substance as can certain critters as well as plants.
I think it was Carrie who was interested in the Mussel's glue.
Need to keep this thread going. Lots of great information here and would be a shame to have it end up buried in the archives.
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Post by teller on Jan 12, 2008 20:57:31 GMT -5
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Post by skytroll on Jan 13, 2008 11:49:52 GMT -5
Toni, I do believe the X in the insects, is similar to X in humans. Women have 2, the carrier gene X that is dormant for diseases women carry, their children would get, is what I believe they called "junk DNa" Because it is not active in the carrier, but the descendents get the carried disease. Now if this infects insects X then it could infect humans. This is a new oncho worm described here and seems to be the evolutionary link between worms and bacteria? They are saying is symbiont meaning can live in the host, but not nec change DNA. However, if this effects the insects DNA, could effect ours. Dr. Harvey's theory may be our best one yet, in relation to the oncho. Some of us could have this "subassembly" of evolution in us which would change our evolution, specificly. Only those with mutations already on the X. Does that make sense: This article talks of the infection w/ humans: Vet Parasitol, 2002 Sep 10, 108(2), 153 - 61 Molecular phylogenetic analysis of Onchocerca lupi and its Wolbachia endosymbiont; Egyed Z et al.; The morphology of Onchocerca lupi, responsible for canine ocular onchocercosis, is unique within the genus . Earlier analyses of the 5S ribosomal RNA gene spacer region sequence of the parasite and the 16S ribosomal RNA gene sequence of its Wolbachia endosymbiotic bacteria (Rickettsiales) supported the morphological and biological arguments that O . lupi is a distinct species . However, the exact phylogenetic position of O . lupi and its endosymbiont could not be unambiguously determined . Herein we report analyses based on the mitochondrial cytochrome oxidase I (COI) gene of the filarial species and the Wolbachia surface protein (wsp) and the bacterial cell-cycle ftsZ genes of their wolbachiae . Our results indicate that O . lupi separated from other Onchocerca spp . early in evolution . This is in line with the previous morphological analysis demonstrating that O . lupi is an atypical Onchocerca species showing both primitive and evolved characters . The phylogenetic trees generated for the COI sequences of filariae and the wsp and ftsZ sequences of their wolbachiae were congruent with each other, which supports the hypothesis that nematodes and their Wolbachia endobacteria share a long co-evolutionary history." www.bionewsonline.com/s/1/bacteria_i.htmWill look at this onchocerca lupi, Skytroll
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Post by ruth on Jan 13, 2008 12:12:51 GMT -5
Maintenance and killing efficiency of conditional lethal constructs in Pseudomonas putida. Bej AK, Molin S, Perlin M, Atlas RM. Department of Biology, University of Louisville, Kentucky 40292.
Conditional lethal (suicidal) genetic constructs were designed and employed in strains of Pseudomonads as models for containment of genetically-engineered microbes that may be deliberately released into the environment. A strain of Pseudomonas putida was formed with a suicide vector designated pBAP24h that was constructed by cloning the host killing gene (hok) into the RSF1010 plasmid pVDtac24 and placing it under the control of the tac promoter. After hok induction in P. putida only 40% of surviving cells continued to bear the hok sequences within 4 h of induction; in contrast, 100% of the cells in uninduced controls bore hok. A few survivors that demonstrated resistance to hok-induced killing developed in P. putida, which may have been due to a mutation or physiological adaptation that rendered the membrane 'resistant' to hok. Conditional lethal strains of P. putida also were formed by inserting gef (a chromosomal homolog of hok) under the control of the tac promoter into the chromosome using a transposon. Constructs with chromosomal gef, as well as an RK2-derived plasmid construct containing gef, were only marginally more stable than the hok constructs; they were effective in killing P. putida when induced and within 2 h post-induction killing from either gef construct resulted in a 10(3)-10(5)-fold reduction in viable cell count compared to uninduced controls.
PMID: 1368479 [PubMed - indexed for MEDLINE]
Related Links A substrate-dependent biological containment system for Pseudomonas putida based on the Escherichia coli gef gene. [Appl Environ Microbiol. 1993] Model suicide vector for containment of genetically engineered microorganisms. [Appl Environ Microbiol. 1988] Dual system to reinforce biological containment of recombinant bacteria designed for rhizoremediation. [Appl Environ Microbiol. 2001] Characterization of cell lysis in Pseudomonas putida induced upon expression of heterologous killing genes. [Appl Environ Microbiol. 1998] Antimicrobial properties of the Escherichia coli R1 plasmid host killing peptide. [J Biotechnol. 2003] See all Related Articles... Display SummaryBriefAbstractAbstractPlusCitationMEDLINEXMLUI ListLinkOutASN.1Related ArticlesCited in BooksCancerChrom LinksDomain Links3D Domain LinksGEO DataSet LinksGene LinksGene (GeneRIF) LinksGenome LinksProject LinksGENSAT LinksGEO Profile LinksHomoloGene LinksCoreNucleotide LinksCoreNucleotide (RefSeq) LinksEST LinksEST (RefSeq) LinksGSS LinksGSS (RefSeq) LinksNucleotide LinksNucleotide (RefSeq) LinksOMIA LinksOMIM (calculated) LinksOMIM (cited) LinksBioAssay LinksCompound LinksCompound (MeSH Keyword)Compound (Publisher) LinksSubstance LinksSubstance (MeSH Keyword)Substance (Publisher) LinksPMC LinksCited in PMCPopSet LinksProbe LinksProtein LinksProtein (RefSeq) LinksProtein Cluster LinksCited ArticlesSNP LinksStructure LinksTaxonomy via GenBankUniGene LinksUniSTS Links Show 5102050100200500Sort ByPub DateFirst AuthorLast AuthorJournalSend toTextFilePrinterClipboardE-mailOrder
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Post by toni on Jan 13, 2008 12:51:53 GMT -5
Ruth, I'm adding this in right now (modifying the post) because I hadn't seen your post there before now. Yes, the Putida is in the good ole GMO too....seems it all is (that we have going on) www.answers.com/topic/pseudomonas-putidaWhich leads to: Polyhydroxyalkanoates or PHAs below. Polyhydroxyalkanoates or PHAs are linear polyesters produced in nature by bacterial fermentation of sugar or lipids. More than 100 different monomers can be combined within this family to give materials with extremely different properties. Pseudomonas putida CA-3 is capable of converting the aromatic hydrocarbon styrene, its metabolite phenylacetic acid, and glucose into polyhydroxyalkanoate (PHA) when a limiting concentration of nitrogen (as sodium ammonium phosphate) is supplied to the growth medium. Accumulation of Polyhydroxyalkanoate from Styrene and Phenylacetic Acid by Pseudomonas putida www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1082534They can be either thermoplastic or elastomeric materials, with melting points ranging from 40 to 180 °C. The most common type of PHA is PHB (poly-beta-hydroxybutyrate). PHB has properties similar to those of polypropylene, however it is stiffer and more brittle. To produce PHB a culture of a micro-organism such as Alcaligenes eutrophus is placed in a suitable medium and fed appropriate nutrients so that it multiplies rapidly. Once the population has reached a substantial level, the 'diet' is changed to force the micro-organism to create PHB. www.answers.com/topic/polyhydroxyalkanoatesAlcaligenes eutrophus is a bacterial species that naturally produces polyhydroxyalkanoates (PHA). PHA's are a broad type of biodegradable polymers that can be used for biodegradable plastics. A. eutrophus specifically produces polyhydroxybutyrate (PHB), which it uses for storing carbon when in an environment with abundant carbon, but limited essential nutrients such as nitrogen or phosphorus. The gene for PHB production has been cloned and engineered into plants. en.wikipedia.org/wiki/Alcaligenes_eutrophus www.answers.com/topic/alcaligenes-eutrophus
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Post by toni on Jan 13, 2008 12:56:46 GMT -5
Skytroll, OH YES! and I've read that about the X chromosome, and I certainly see where Dr. Harvey got his "DNA info" and it makes sense. And the "junk DNA"...oh boy, that fits too. Here's more on the PHAs. Many individuals have acknowledged the benefits that you receive from a compost pile. However, there are several things that will not degrade. One of which is styrofoam. There are plastics that can be degraded. PHA, for example, is the polymer of hydroxyalkanoates that is accumulated as a carbon and energy storage material in various microorganisms. PHA has been developed using the bacterium Alcaligenes eutrophus. A. eutrophus is used because of its ability to oxidize hydrogen gas for energy. It is a gram-negative, rod shaped bacterium. It thrives in the presence of toxic heavy metals. web.mst.edu/~microbio/BIO221_2003/alcaligenes_eutropha.htm
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Post by toni on Jan 13, 2008 13:43:18 GMT -5
Scientists have just now realized the importance of A. eutrophus’s ability to produce plastic and are trying to harness this power. Many researchers are now growing mass cultures of A. eutrophus in conditions that promote PHB production and extracting the plastic. Others are using genetic engineering to insert the DNA sequences of the three enzymes discussed above into the DNA of the plant Arabidopsis thaliana, thus growing plants able to synthesize plastics. web.mst.edu/~microbio/BIO221_2005/A_eutrophus.htm
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