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Post by bannanny on Jun 27, 2009 23:09:46 GMT -5
I know our sweet Robbie was diagnosed with Pseudomonas aeruginosa. Well, kammy's been diagnosed with it in her ears and she also identified it in my samples that she's been culturing. I think maybe we should all be tested for it if we can. I don't even know that much about it yet, but kammy will be adding to this thread when she can. emedicine.medscape.com/article/226748-overviewExcerpt from above link... [glow=red,2,300]Pseudomonas is a gram-negative rod that belongs to the family Pseudomonadaceae. More than half of all clinical isolates produce the blue-green pigment pyocyanin. Pseudomonas often has a characteristic sweet odor. These pathogens are widespread in nature, inhabiting soil, water, plants, and animals (including humans). Pseudomonas aeruginosa has become an important cause of infection, especially in patients with compromised host defense mechanisms. It is the most common pathogen isolated from patients who have been hospitalized longer than 1 week. It is a frequent cause of nosocomial infections such as pneumonia, urinary tract infections (UTIs), and bacteremia. Pseudomonal infections are complicated and can be life threatening.[/glow] It's another plant pathogen too. I think we should add it as part of the morg mix. What do you think? hugs ~~ bannanny  |
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Post by kammy on Jun 28, 2009 8:14:31 GMT -5
I know our sweet Robbie was diagnosed with Pseudomonas aeruginosa. Well, kammy's been diagnosed with it in her ears and she also identified it in my samples that she's been culturing. hugs ~~ bannanny Hi Banny, I just wanted to clarify that we are in the beginning, discovery phases of how major a 'player' that pseudomonas aeruginosa 'P.a.' is in Morgellons. I was lucky, in that, I was able to isolate it in my Petri Dish so that it became very evident for me and my doctor to see/identify, smell and verify. (It has a distinct odor.) We are not sure at this time IF what we were calling 'Peridiscaceae' the white speck starter 'seeds' - the spheres - are indeed P.a., also, I believe it has been mis-identified with that name? We're still debating and running daily experiments to watch P.a. going through it's step-by-step growing process. You notice that P.a. is described as a 'rod-shaped' bacteria. However, we have an abstract that shows that it is sphere-shaped with its two mutant strains, which is what we believe we might be dealing with? I am almost positive that the biofilm that my bacteria is emitting is trapping other pathogens inside of it by the numerous photographs that I have - that show this happening. The biofilm is like a glue that anything that comes along can use as a womb or incubator with the protection that a biofilm allows. IMO, this is what is making Morgellons symptoms to get rid of completely, this bacteria is very resilient and antibiotic immune. That unless you take a quorum sensing antibiotic, you will not phase this! I made the statement that you 'might' also have this infection, something to look into, we are not sure yet if this is a common factor with all people with Morgellons - even though, to me - it looks like it is. We have to present this in a scientific way, have others run tests, etc. to make it a fact. |
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Post by kammy on Jun 28, 2009 8:23:21 GMT -5
Howie - we are 'on' this P.a., already. We have some very, very interesting news about this particular bacteria that I will be publishing soon. Kat and I now have the capability to look at the microscopic slides and data BEFORE we publish, to more closely scrutinize what is said.
I have a new, YouTube out that we believe shows this bacteria reproducing and it in several stages. At the beginning, it shows it as like a deflated balloon and behind it - it has left a trail of biofilm and 'babies'. I believe this is just one of many ways this bacteria reproduces, I have many photos of it using various methods of reproduction, these methods have not been verified yet. At the end of the video - it shows an immature coccus seed that is 'sitting' on a more mature 'fuzzier' one that I believe is in a more advanced stage. I did something unusual to my ear to finally get this pathogen to reveal itself in this manner, cultured June 5th, after 6 weeks of diflucan (no fungus present), and one week of Levaquin - only 2 or so 'fibers' in the dish, this video represents 3 weeks growth, photographed at 300x:
Howie - How did YOU come to your conclusion that this bacteria might be involved?...
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Post by toni on Jun 28, 2009 8:28:08 GMT -5
Kmarie, Yes, (Ginseng) has been and is being studied for the treatment of P.A. Last year I think it was I'd read about this, and we also take this supplement (for preventative measures too). Ginseng did what no antibiotic could in the study. www.ncbi.nlm.nih.gov/pubmed/9801351( I don't think me and Mr T have P.A.) judging by the way we feel, we both feel fine and are able to both put in a full days work every day without any problems. |
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Post by kammy on Jun 28, 2009 8:29:52 GMT -5
Pseudomonas aeruginosa OK, what's going on? Are we introducing a new pathogen... no, this guy has been here all along. Due to the nature of what we're dealing with - I'm going to make some mistakes. I'm now thinking that this is what was earlier identified as 'Crypto' and then changed to 'Peridiscaceae'. That we've been looking at a bacteria all this time. Input please? I added an experimental solution to my ear right before I cultured it to see what effect this solution would have on the growth. This caused all the 'others' to be missing, or the medicines I had just taken caused this? and allowed this one main, what I have been calling a 'seed' pathogen to be primary in the dish. This pathogen starts out as a salt crystal. It is an exact match to the wikipedia dish growth photo. That by the other factors being in the dish previously, what it appeared to be visually, (the white circular connected growths), was masked by the other pathogen's influences? Wikipedia photo:  P. aeruginosa on an XLD agar plate. I took this dish to an ENT yesterday, already suspecting P.a. from a visual match, which he verified that it was this pathogen by visual inspection, it has a distinct odor, he said, he looked and seemed very familiar with this pathogen, especially, recognized the odor (smells like a musty basement in the dish) and is treating me with internal and topical ciproflaxin, diflucan and an over-the-counter topical, anti-fungal for athlete's feet. The solution also allowed its distinct odor to be noticeable, which in the past - was not. I have just finished 16 days of Biaxin and Quensyl, in which I cultured once again, prior to the Ciproflaxin, which I just started. The results of the Biaxin/Quensyl culture will be noted, also. To be specific, the ENT did not send my dish to a lab, nor did he culture my ear. I have no lab documentation to verify these findings, he requested a follow-up visit in 3 weeks, I am being treated for a Pseudomonas aeruginosa infection of my ear. |
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Post by kammy on Jun 28, 2009 8:37:27 GMT -5
Hi Toni...
Of course, there are many factors to Morgellons, as we have seen, read and know. We just need something scientifically solid to build on, that all we're trying to do - identify some factor here that is working on us. I'm not saying that P.a. is in everyone, but - here's what I'm suspecting right now:
I think that if what I have is P.a., or something similar... that it is the source for the white AND the black specks. I have to prove these statements, which I am in the process of.
And... if it is... then this is will probably identify the main pathogen because 'specks/seeds' are something we all have in common and then we'll have something concrete to work with and hopefully know more about how and where to look and to help our physicians find our illness.
Another way to say this - if you're producing a biofilm and specks... then P.a. or a variation of it - is probably involved.
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Post by kammy on Jun 28, 2009 8:48:34 GMT -5
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Post by kammy on Jun 28, 2009 8:50:27 GMT -5
QUOTE=Jeany Hey Howie! Thanks! I wanted to add a little more information here about PA..could be that this has been already mentioned before... P. aeruginosa secretes a variety of pigments, including pyocyanin (blue-green), fluorescein ( yellow-green and fluorescent, now also known as pyoverdin), and pyorubin (red-brown). P. aeruginosa is often preliminarily identified by its pearlescent appearance and grape-like or tortilla-like odour in vitro. Definitive clinical identification of P. aeruginosa often includes identifying the production of both pyocyanin and fluorescein, as well as its ability to grow at 42°C. P. aeruginosa is capable of growth in diesel and jet fuel, where it is known as a hydrocarbon-utilizing microorganism (or "HUM bug"), causing microbial corrosion** It creates dark gellish mats sometimes improperly called " algae" because of their appearance. **does this mean PA can survive even in fuel/air mix? Fuel residues in our water systems...??An opportunistic pathogen of immunocompromised individuals, P. aeruginosa typically infects the pulmonary tract, urinary tract, burns, wounds, and also causes other blood infections. It is the most common cause of infections of burn injuries and of the external ear ( otitis externa), and is the most frequent colonizer of medical devices (e.g., catheters). Pseudomonas can, in rare circumstances, cause community-acquired pneumonias,as well as ventilator-associated pneumonias, being one of the most common agents isolated in several studies.
Pyocyanin is a virulence factor of the bacteria and has been known to cause death in C. elegans by oxidative stress. However, research indicates that salicylic acid can inhibit pyocyanin production.One in ten hospital-acquired infections are from Pseudomonas. Cystic fibrosis patients are also predisposed to P. aeruginosa infection of the lungs. P. aeruginosa may also be a common cause of "hot-tub rash" ( dermatitis), caused by lack of proper, periodic attention to water quality. The most common cause of burn infections is P. aeruginosa. Pseudomonas is also a common cause of post-operative infection in radial keratotomy surgery patients. The organism is also associated with the skin lesion ecthyma gangrenosum. It has been found that with low phosphate levels, P. aeruginosa is activated from benign symbiont to express lethal toxins inside the intestinal tract and severely damage or kill the host, which can be mitigated by providing excess phosphate instead of antibiotics.Kam%**, didn't you tell me that the 'sollution' you used on your ear contained phosphates? Maybe this is/was the reason that PA reacted so quickly? like you already said? YES!!With plants, P. aeruginosa induces symptoms of soft rot with Arabidopsis thaliana (Thale cress) and Lactuca sativa (Lettuce). It is a powerful pathogen with Arabidopsis and with some animals: Caenorhabditis elegans, Drosophila and Galleria mellonella. The associations of virulence factors are the same for vegetal and animal infections.
And..did you know that experiments were/are done with PA and nematodes classified as Caenorhabditis elegans?? Flies ? and moths?
And that this type of Nematode mixed with PA was brought along for experiments on the Space Shuttle Columbia which 'crashed' 2003?
A group of small (1 mm adult) Caenorhabditis elegans worms, living in petri dishes enclosed in aluminium canisters, survived re-entry and impact with the ground and were recovered weeks after the disaster. The culture was verified as still alive on April 28, 2003. They were part of a Biological Research in canisters experiment designed to study the effect of weightlessness on physiology. It might mean nothing...but keeps me sort of thinking...Nano gel..remember?Jeany |
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Post by kammy on Jun 28, 2009 8:51:06 GMT -5
ASPIRIN EXPERIMENT "However, research indicates that salicylic acid can inhibit pyocyanin production.One in ten hospital-acquired infections are from Pseudomonas." When I was reading up on PA, I read this so, I took a small glass of water that my ear would fit into exactly and put it in the microwave for 1 minute and made the water hot, put 4 aspirins in the water, dissolved it and took 2 aspirins at the same time - let it cool down and then soaked my ear in this glass of water for as long as I could hold this position, approx. 10 minutes. My ear showed improvement in this short amount of time. I haven't repeated this experiment, I only did it once - I am now putting these other medicines on it. We might want to try some aspirin/water solution testing to see if this helps with lesions? The Andromeda Strain? |
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Post by kammy on Jun 28, 2009 8:52:11 GMT -5
QUOTE=Jeany These pictures look quite similar to what shows in Kam's dish:  
The Same but Different: 30 times magnified image of colonies from four different strains of the bacterium Pseudomonas aeruginosa (a human pathogen under some conditions) as it is grown under routine laboratory conditions.
Even though they are all the same bacteria it is clearly visible how the bacteria have adapted their morphology to survive in their respective environments.
Jeany
[/QUOTE] |
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Post by kammy on Jun 28, 2009 8:53:36 GMT -5
Quote=Jeany
Gosh Kam**, when I look at your video, doesn't it look like those 'fibers' are somehow attached/integrated to/in the bacteria...like they seem to feed on it?
Jeany
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Post by kammy on Jun 28, 2009 8:54:00 GMT -5
Of course, this one pathogen isn't all there is... from what we've seen - everything/anything can get 'caught up in' this biofilm PA emits. We know that the key to getting well is to eliminate the biofilm, it's the most difficult part of this disease to rid. It's the womb for everything else!
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Post by kammy on Jun 28, 2009 8:54:56 GMT -5
QUOTE=Jeany Is this another aspect to consider? Research could lead to new non-antibiotic drugs to counter hospital infections | The University of ChicagoLack of an adequate amount of the mineral phosphate can turn a common bacterium into a killer.... Clinicians have long known that after an operation or organ surgery, levels of inorganic phosphate fall.
The authors of the PNAS paper, led by scientists at the University of Chicago, hypothesized that phosphate depletion itself signals P. aeruginosa to become lethal when present in the intestinal tract of a stressed host. To test this theory, they let worms (Caenorhabditis elegans) feed on “lawns” of P. aeruginosa and Escherichia coli grown in both low-phosphate and high-phosphate media. What about this? Could it be that insects/worms sort of morph after contact with PA? Is here the water connection too?Only the worms that ate P. aeruginosa with low levels of phosphate died. The researchers dubbed the phenomenon “Red Death” since unexpected large red spots appeared inside the digestive tube of worms before they died.  When worms (Caenorhabditis elegans) ate the bacteria Pseudomonas aeruginosa that were raised on low levels of phosphates, unexpected large red spots appeared in their digestive tubes.The worms then died, so researchers dubbed the condition “Red Death.” They theorized that providing P. aeruginosa with phosphate would protect weakened or immunosuppressed hospital patients from this lethal pathogen. So...high levels of phosphates is the clou?? hmm...Jeany |
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Post by kammy on Jun 28, 2009 8:56:04 GMT -5
Originally Posted by Jeany
"Gosh Kam**, when I look at your video, doesn't it look like those 'fibers' are somehow attached/integrated to/in the bacteria...like they seem to feed on it?
Jeany"
Yes. There's some serious quorum sensing going on. We read how this bacteria is so antibiotic resistant - that we must take one of those quorum sensing ones listed in order to rid this! This isn't any ordinary bacteria, this is a mean one!
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Post by kammy on Jun 28, 2009 8:57:44 GMT -5
quote=Jeany These pictures look quite similar to what shows in Kam's dish:
The Same but Different: 30 times magnified image of colonies from four different strains of the bacterium Pseudomonas aeruginosa (a human pathogen under some conditions) as it is grown under routine laboratory conditions.
Even though they are all the same bacteria it is clearly visible how the bacteria have adapted their morphology to survive in their respective environments.
Jeany
This is an earlier photo that looks like a match to the one on the bottom left: [/IMG] "Fried Egg" below: ![]() my-stuff-dot-com.com/My Stuff/Personal/Morgellons/My Photos/Master/Experiment 3/5 3 09 4 18/05_04_83.JPEG[/img][/CENTER] |
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Post by kammy on Jun 28, 2009 8:59:16 GMT -5
PA with the solution, cultured 6/5, human sample @100x - [/img] ![]() my-stuff-dot-com.com/My Stuff/Personal/Morgellons/My Photos/Experiment 1/6 26 09 - 6 5/06_26_22.JPEG[/img][/CENTER] |
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Post by kammy on Jun 28, 2009 9:00:14 GMT -5
QUOTE=Kammy I believe this is PA again?, (without the solution) taken 3/24/09, human sample @100x. Earlier we saw this photo and remarked how it resembled a Lyme bacteria? [/img][/CENTER] |
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Post by kammy on Jun 28, 2009 9:01:12 GMT -5
QUOTE=Kammy If I had all the photos lined up, I believe I could show how this sphere below... ![]() my-stuff-dot-com.com/My Stuff/Personal/Morgellons/My Photos/Master/Experiment 3/5 3 09 4 18/05_04_33.JPEG[/img] eventually turns into this: ![]() my-stuff-dot-com.com/My Stuff/Personal/Morgellons/My Photos/Master/Experiment 2/3 29 Exp 2/03_29_21.JPEG[/img] then to this: ![]() my-stuff-dot-com.com/My Stuff/Personal/Morgellons/My Photos/Master/Experiment 2/3 26 09 Left Ear Exp 2/03_27_85 L Ear 100x Exp 2 - 2 28.JPEG[/img] |
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Post by kammy on Jun 28, 2009 9:01:47 GMT -5
What do you think, Jeany*? Am I close...? Input anyone... we need help figuring this out?
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Post by kammy on Jun 28, 2009 9:02:52 GMT -5
Originally Posted by Kammy
I believe this is PA again, (without the solution) taken 3/24/09, human sample @100x. Earlier we saw this photo and remarked how it resembled a Lyme bacteria?
Quote = Jeany
Not sure about this one Kam**, you say it's PA?...
I would say it's Aspergillus Niger like we identified before..those black spores and hyphae are significant for this type of fungi.
We need to do more tests....maybe it would be helpful to check on the cultures daily? take pictures in order to see how it changes it's appearance after a period of time?
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