Pseudomonas aeruginosa

[kammy]

Good idea, Jeany... it's just too hard to say... what all is happening except that I think we're getting closer...?

It's there for whoever wants to put in their 2 cents worth.

[kammy]

quote=Kammy;

I believe this is PA again?, (without the solution) taken 3/24/09, human sample @100x. Earlier we saw this photo and remarked how it resembled a Lyme bacteria?

my-stuff-dot-com.com/My Stuff/Personal/Morgellons/My Photos/Experiment 1/3 24 09/03_24_127.JPEG

[/img][/CENTER]


Well... well, WTG! Howie! Tendrils... yeah, if we could get a microbiologist to look at that above... we could possibly make the Morgie/Lyme connection? I believe P.a. has some 'sisters'? They are both similiar-looking bacteria. (We need the photo from the Lymes source that's similar to it, I'm sure it's been named?)

So, it's possible that the photo above could be bacteria and not a fungus? That's one in favor... thanks, Howie.

Here it is:

quote=Kammy

Morgellons and Lyme's are Sister Diseases

THESE ARE THE 'BLACK SPECKS' @100x:

my-stuff-dot-com.com//My Stuff/Personal/Morgellons/My Photos/4-24-09 4 18/04_24_21.JPEG[/img]

Lyme Disease Bacterial Colony
This Colony Contains All Three Bacterial Forms:
Spirochete, Cyst, and Cell-Wall-Deficient

They appear to be very close family members - somehow?


Ok, you Lyme specialists... here's something to think about?

[kammy]

P. aeruginosa is typically described as a 'rod-shaped' bacteria. The second abstract below speaks of its two mutant strains, PAO1 and PBP2, that are sphere-shaped.

Pseudomonas

"P. aeruginosa isolates may produce three colony types. Natural isolates from soil or water typically produce a small, rough colony. Clinical samples, in general, yield one or another of two smooth colony types. One type has a fried-egg appearance which is large, smooth, with flat edges and an elevated appearance. Another type, frequently obtained from respiratory and urinary tract secretions, has a mucoid appearance, which is attributed to the production of alginate slime. The smooth and mucoid colonies are presumed to play a role in colonization and virulence."

------------------------------------------

Function of penicillin-binding protein 2 in viability and morphology of Pseudomonas aeruginosa -- Legaree et al. 59 (3): 411 -- Journal of Antimicrobial Chemotherapy

"Results: PAO1 grew with a spherical morphology in the presence of mecillinam at concentrations as high as 2000 mg/L.

The PAO1 {Delta}pbpA insertional mutant also grew as spheres, and complementation with a plasmid encoding active pbpA, but not with an inactive Ser-327.

With P. aeruginosa at this concentration of mecillinam, cells retained their normal rod shape but, surprisingly, a morphological change to spherical cells was observed when incubated at concentrations between 200 mg/L and 400 mg/L (Figure 2).

Characterization of PBP 2

To ensure that the proteins being investigated were indeed either the full-length or truncated PBP 2 from P. aeruginosa, N-terminal sequence analysis and mass-spectrometry fingerprinting were performed. The N-terminal amino acid sequences obtained corresponded to those expected for each protein (Figure 1), and the MS-Fit42 gave a MOWSE score of 3.3 x 107, thus confirming the identity of P. aeruginosa PBP 2.

PAO1 {Delta}pbpA mutants lacking a functional pbpA gene were observed to grow in LB broth at 37°C, albeit at an apparent slower rate compared with wild-type PAO1 cells (Figure 7). SEM analysis revealed that, like wild-type cells treated with mecillinam, the PAO1 {Delta}pbpA mutant grows with a spherical morphology, although perhaps with a little less uniformity in appearance (Figure 7). Normal rod-shaped morphology and cell dimensions were restored upon transformation of the mutant with the complementation vector pACBL31 which contained a functional copy of pbpA."

[toni]

Hi Kammy,

Here's where I think some things I say may get misconstrued.

Gosh...(in typing you know how things don't sound the same and saying them in person?)

That is what I find myself up against, if you will...when I make comments.

So to let you know, ( I don't speak for everyone-only our experiences ) for instance when I say me and Mr T take Ginseng and we're fine in the P. A. department, that's only because (he and I are), and I'm only speaking about the Ginseng part, and the P.A. part (for the two of us), from what we're experiencing.

But we're no way "cured" of Morgellons.

I believe Morgs is multi-faceted, and each and every one of us, is more susceptible then to (one of the many pathogens) we've received in the "Morgs cocktail"....basically.

I know that Klebsiella (now I've drawn a blank on the other) bacterial pathogen that a friend of mine lost her life to.

I think we have ALL these pathogens! Many many pathogens. And depending on "what pathogen" we're more *susceptible* to in our body...the more it will grow out of control. And that is also imho, where we all differ.

Sort of like if I had 5 bacterial pathogens.
And you have 5 bacterial pathogens.
Lets say we both have the same 5 strains.

Now lets say you take Tetracycline, and I take Bactrim.

What now is going to happen, is you'll rid one of those bacterium's, and I'll then rid another.

That's what I mean. Because you and I then are targeting "particular bacterium's" with what we're treating ourselves with....but we cannot both rid the same things, because we both aren't taking the same thing.

I know many have P.A. because they've had tests done to determine this.

One thing I'd read about last year, re: P.A. is, once it gets out of control in the body...there is very little chance of turning it around or stopping it. It can become easily "out of control" and then is lethal in more cases than not.

No meds to date then can cause the P.A. to become sensitive.
So that is why I was very adamant about (this study on the Ginseng) because imho, it was important for all us too, to consider, since it is a proven piece of the puzzle.

[kammy]

Quote:
Originally Posted by Kammy

Well... well, WTG! Howie! Tendrils... yeah, if we could get a microbiologist to look at that above... we could possibly make the Morgie/Lyme connection? I believe P.a. has some 'sisters'? They are both similiar-looking bacteria. (We need the photo from the Lymes source that's similar to it, I'm sure it's been named?)

So, it's possible that the photo above could be bacteria and not a fungus? That's one in favor... thanks, Howie.

Ok, you Lyme specialists... here's something to think about?

Quote = Jeany

Tendrils? well..ok...not so sure about that....

Didn't we say a time back ago and it has been proven by asking my Lyme Doc that it is not possible to 'see' Lyme Bacteria at that microscopical level 100x? It takes at least 1000x and requires a special technique to show evidence. And if it would be that 'easy' to recognize WHY are these Lyme tests so difficult if it would only take to set up a culture, wait a few days and there you have it? NO, I don't think so.

But Fungi? YES...everyone knows that Fungi can be easily seen even with the naked eye...and in this case with 100x even better. IMO..this picture is showing Fungi, Aspergillus Niger, with typical hyphae growth.

Besides that didn't we say, according our research, that we assume Morgellons Disease is a multi infectious disease with several pathogens?
And that there must definitely be Fungi involved? Do you think that you were able to separate the fungi from bacteria while taking debris from your ear to set up a culture? NO, I don't think so.

IMO...what we are seeing here is PA, yes...as your doc indentified also...good...but the other specimen is AN.

And here is something for you to think about: How come so many people who are infected with Morgellons Disease seem to have problems with mold or fungi in their environments and show typical symptoms of fungal infections? And after antifungal treatments such as Diflucan or Itraconazole...like in my case and yours...the symptoms are decreasing? like reduction of the black specks and itching? hmm,..WHY?

AND...didn't we say Bacteria feeds on Fungi and vice versa? and that's the reason why Morgs aren't easy to eliminate? That it take BOTH meds in order to 'kill' them?

Like I already said, we need to do more tests..with daily observation. I was just thinking here..maybe we should set up a new culture and add antifungal components and watch and see what happens? If it's a bacteria then it's still there...if it's a fungi then...well...atleast the 'growth' should be inhibited?

Another point: ALL insects are attracted by bacteria...not only PA...open wounds are most likely to be infested by insects..that could be the connection to parasites..but has IMO not explicit something to do with PA only.

Folks, it's not that easy!


Jeany

[kammy]

Quote = Jeany


Hi Howie,

please let me say how much your input is appreciated here!Thank you!
Yes, you are definitely right about how bacteria attracts all sorts of insects..not only that but fungi and environmental particles get even 'caught' in this sticky biofilm PA is producing...and of course also insects causing Lyme Disease. The point is though that most specialists working on Lyme Disease aren't totally assure if insects only are the causative agent of Lyme's in the first place. What we believe is, that it not only takes insects to trigger this but also other toxins such as in water...as you mentioned correctly...play a main role in Morgellons Disease. And if you think about bio remediation...YES..using Nanobacteria...mutated, morphed strands of other bacteria such as PA...well...this also IMO leads to an super infectious disease not only Morgellons Disease but many other illnesses perhaps even all related to each other.

The overuse of pesticides, insecticides, herbicides and other highly toxic substances not to mention overuse of antibiotics leaching into our water systems cause these resistant bacteria strands to mutate..and also to speak ahead here..fungi, algae and other water organisms.

btw...I have also noticed this 'powdery' substance on my skin and hair after taking a shower..and tap water is a big NO NO also in my case. And I totally agree with you by using Neem soap and/or hairtonic.. and....I use it internally also....It not only removes the biofilm but also 'fights' fungi, bacteria and parasites...so...this stuff is great!

[kammy]

Ok, here's where I'm at:

I need to isolate a white and black speck in the dish, photograph them daily, map the dish so that I am photographing the same spot day after day and show the life-cycle progress of these specks.

I am taking the Ciproflaxin and can already tell that the debris is changing, my ear is responding. I may not have any specks soon (I pray)... so, I need to gather this evidence while I'm still producing. I cultured a dish right before I took the Cipro, which I photographed yesterday, that can be a backup dish.

I shouldn't have brought in the Lyme/Morgie connection, I was going through the photos looking for good examples and saw that one again... and thought I'd just put it out here. Who knows, someone who is interested maybe could use this information?

To keep things simple - let's just concentrate on the P.a. issue at hand?

[toni]

This is the reason Ginger is treating her patients with (a cocktail of meds ) because she's longed believed it's muti faceted.

I also took Itraconazole and Fluconazole (alternated them both for 9 months) . In my experience only, these didn't stop my black specks. (just to let you know).

[kammy]

Jun 28, 2009 9:21:42 GMT -5 toni said:

This is the reason Ginger is treating her patients with (a cocktail of meds ) because she's longed believed it's muti faceted.

I also took Itraconazole and Fluconazole (alternated them both for 9 months) . In my experience only, these didn't stop my black specks. (just to let you know).

Yes, Toni - we're aware this is a multi-faceted disease and have stated that we're seeing fungi involved in most people's samples, so far. This is only from a small pool of different human cultures. I have 3 more people's samples that I am about to culture soon.

We know that P.a. is a wicked bacteria and if the fungi is getting inside the biofilm, that this isn't a safe place for anything to be when drugs come along to fight it. We've felt the stickiness of our biofilm goo... ??

I don't know whether to continue with the nutrient agar or switch to the potato dextrose agar, which I don't have any experience in. I was trying to get away from the marine elements (nutrient agar is made from seaweed) to determine if we are or are not identifying the components of nutrient agar? To take that variable out of the picture. However, I have a limited amount of specimens and would hate to culture them in something that may not be productive.

[toni]

Here's another article on the Ginseng (you were saying Neem) but that's not what I was saying.

www.fiu.edu/~matheek/alternatetherapy.htm


I personally haven't gotten into culturing, (which isn't my thing), but I'm glad you are.

Just a thought here Kammy. I do understand about the seaweed agar-but that's as far as I go with it, but...(just for a tester) and I may not know what I'm talking about, what about using a saline solution on some of the specimens? (just to see what happens?)

[kammy]

I know that phosphates are a key in fighting this disease, Jeany... phosphorus. Yes, I used a mild home-made solution that contained phosphates, which I don't believe are supposed to be put on the skin? This is not recommended.

I recommended the LOW phosphorus diet on the 'Cure Thread' based on the metals were were finding at that time - you mean all that cheese and milk I was craving was a good thing - that we need a HIGH phosphorus diet?

[kammy]

Jun 28, 2009 9:39:59 GMT -5 toni said:

Here's another article on the Ginseng (you were saying Neem) but that's not what I was saying.

www.fiu.edu/~matheek/alternatetherapy.htm


I personally haven't gotten into culturing, (which isn't my thing), but I'm glad you are.

Just a thought here Kammy. I do understand about the seaweed agar-but that's as far as I go with it, but...(just for a tester) and I may not know what I'm talking about, what about using a saline solution on some of the specimens? (just to see what happens?)

Hey Toni, this Ginsing information is very important to the other folks who might be tuning in on the other site.

Are you over there to post, or do you mind me sharing this information with them?

[kammy]

Jun 28, 2009 9:39:59 GMT -5 toni said:

Here's another article on the Ginseng (you were saying Neem) but that's not what I was saying.

www.fiu.edu/~matheek/alternatetherapy.htm


I personally haven't gotten into culturing, (which isn't my thing), but I'm glad you are.

Just a thought here Kammy. I do understand about the seaweed agar-but that's as far as I go with it, but...(just for a tester) and I may not know what I'm talking about, what about using a saline solution on some of the specimens? (just to see what happens?)

First of all, if P.a. is what's acting on us - you must see your doctor and get a quorum sensing antibiotic! Remember that the quorum sensing antibiotics work on the same enzyme that allows communication for life as the fungi uses - it should kill the fungi too.

That any 'solution' that I or anyone else comes up with will only treat this topically and this needs to be fought internally or if there is an herb strong enough to kill this... We see that it can grow in gasoline?

I have been dousing myself with neem and taking it internally, along with non-quorum sensing antibiotics with little results - however, ginsing is the one thing I have not tried. I have not been as diligent with the neem as I could be, to be fair.

Let us say to add ginsing to our protocols to see what difference it might make?

[toni]

Kammy,

All the info I find is for everyone. Share it any where you like.

(not that this is key) but I want to help by letting you know what I've done (diligently) ONLY because it might help you somewhere in your tests.

I did Neem diligently for 2 months without fail. Internally with supplements, and topically with the oils, and the creams, shampoos and soaps.

I'm not saying they don't work....but personally...I just found them (okay), but I sure didn't get that "well being" from them, like I was making progress, as much as the Ginseng provided.

Supplements take time (as they're cumulative) and not like meds where we pop a few days worth and they work. Supplements in the body are very slow yet progressive - if one stays consistent.

PS...I drink and eat milk and cheese almost daily. Personally I've found "milk" topically as I've talked about for 4 years now, to be extremely beneficial in ridding or "drawing out" the biofilm when used topically.

That's why I mix it with "plain" yogurt...basically to thicken it, as it won't stay put alone.

When milk and plain yog are mixed together, and globbed ontop the head (where there is no sign of infection whatsoever) on the scalp...AFTER it becomes *bone dry* after being left on (undisturbed) for hours...there will be black specks that have been drawn up into the now dried (mixture) that is on the scalp.

If you try this, view the crystals that form on the scalp (after this mixture has become bone dry) ...and you'll see...the black specks will be throughout the dried mixture you've applied topically on your scalp.

[kammy]

Ok, Toni, TY - I'm going to take your recipe over and share it with the others?


So, Toni - have you had your P.a. diagnosis confirmed?

[kammy]

Toni, I'm quoting you again, in case they missed it:

QUOTE=Toni

GINSENG

"Yes, (Ginseng) has been and is being studied for the treatment of P.A.

Last year I think it was I'd read about this, and we also take this supplement (for preventative measures too).

Ginseng did what no antibiotic could in the study."

Effects of ginseng treatment on neutrophil chemilu...[Clin Diagn Lab Immunol. 1998] - PubMed Result


One thing I'd read about last year, re: P.A. is, once it gets out of control in the body...there is very little chance of turning it around or stopping it. It can become easily "out of control" and then is lethal in more cases than not.

No meds to date then can cause the P.A. to become sensitive.
So that is why I was very adamant about (this study on the Ginseng) because imho, it was important for all us too, to consider, since it is a proven piece of the puzzle."

[kammy]

Jun 28, 2009 9:21:42 GMT -5 toni said:

I also took Itraconazole and Fluconazole (alternated them both for 9 months) . In my experience only, these didn't stop my black specks. (just to let you know).

This supports my suspicion that the black specks are the P.a. specks, as per the earlier discussion by me and Jeany on the Lyme bacteria issue above. I am pretty sure the 'seed' white speck is P.a. or close family member, that has been mis-named a couple of times.

Note: There are two different white/crystal specks. Here's an example of the other one, that I don't believe is P.a., not sure yet - that I photographed today:

QUOTE=Kammy

Experiment 2 - "White Salt Crystals"

Arm Lesion - cultured on 5/13/09, photographed on 6/29/09 @100x:


my-stuff-dot-com.com/My Stuff/Personal/Morgellons/My Photos/SB/6 29 09 5 13/06_28_17.JPEG[/img]

my-stuff-dot-com.com/My Stuff/Personal/Morgellons/My Photos/SB/6 29 09 5 13/06_28_36.JPEG[/img]

my-stuff-dot-com.com/My Stuff/Personal/Morgellons/My Photos/SB/6 29 09 5 13/06_28_38.JPEG[/img]

my-stuff-dot-com.com/My Stuff/Personal/Morgellons/My Photos/SB/6 29 09 5 13/06_28_39.JPEG[/img]


This dish is 6 weeks old and has dried out, these appear as small salt specks in the dish.

[kammy]

I didn't get a chance to get to know, Robbie. I hear that she was diagnosed with P.a.? I'm sorry to hear about her passing.

[toni]

Great pics Kammy.

This video shows instantly one drop of milk on a specimen and how easily "milk" will separate whatever this stuff is.

When the milk is left on...the specimens totally disintigrate...(except the black specks will remain).

[jeany]

WOW! Toni! Great video...so milk seems to dissolve PA, ...if it is PA, huh? and the black specks still remain? hmm...maybe cuz they are fungus particles...

Oh, I know..me and my fungi...LOL

What do you think this specimen is?

Thanks btw for participating here!
Jeany