Post by ruth on Mar 13, 2007 19:54:18 GMT -5
centralfloridaresearch.com/lab/
Information
Dear Physician:
Central Florida Research, Inc. is replacing the laboratory operations of Bowen Research and Training Institute, Inc. Bowen Research and Training Institute, Inc. will continue providing Bowen Therapy as a separate and distinct corporation.
The new Borrelia burgdorferi antigen test Central Florida Research, Inc. will be offering is a much more definitive test than the Western Blot. A Borrelia burgdorferi fluorescent antibody is used to detect the antigen in whole blood. The test is set up manually and read by Flow Cytometry.
The Flow Cytometer can count the number of organisms in 100,000 events in 2 minutes and 50,000 in 1 minute. To visually count the organisms in 100,000 events or 50,000 events using a microscope would be almost an impossibility. The Flow Cytometer counts the number of all events passing through the aperture and enumerates the organisms that react with the antibody. The test result will be reported as a percent of the counted events.
This test detects the antigen or spirochete in the blood. The antigen may not be present in the sample tested if there are very few spirochetes within the blood or if they are in some other organ of the body. Therefore, a negative test does not mean the patient does not have Lyme disease. It means that we were unable to detect Borrelia burgdorferi in the specimen.
The test was compared using our instrumentation and the instrumentation of our Flow Cytometer supplier. The test also was compared using Western Blot. However, this could not be used exclusively, as the Western Blot has only a 60% to 70% accuracy for Lyme disease. Data on 56 of the samples evaluated showed 97% of the tests that tested negative by Flow Cytometry were negative by Western Blot. 86% of the Western Blot positive tests were positive by Flow Cytometry. Only 29% of the tests positive by Flow Cytometry were positive by Western Blot. This was expected because the antibody may not be present in a person with Lyme disease.
Borrelia burgdorferi Direct Fluorescent Antibody by Flow Cytometry should be ordered with Wampole PreVue for best diagnostic results.
We feel this test will be a valuable aid for the physician in the diagnosis of Lyme and other diseases when used with symptoms and other tests for Borrelia burgdorferi.
Information
Dear Physician:
Central Florida Research, Inc. is replacing the laboratory operations of Bowen Research and Training Institute, Inc. Bowen Research and Training Institute, Inc. will continue providing Bowen Therapy as a separate and distinct corporation.
The new Borrelia burgdorferi antigen test Central Florida Research, Inc. will be offering is a much more definitive test than the Western Blot. A Borrelia burgdorferi fluorescent antibody is used to detect the antigen in whole blood. The test is set up manually and read by Flow Cytometry.
The Flow Cytometer can count the number of organisms in 100,000 events in 2 minutes and 50,000 in 1 minute. To visually count the organisms in 100,000 events or 50,000 events using a microscope would be almost an impossibility. The Flow Cytometer counts the number of all events passing through the aperture and enumerates the organisms that react with the antibody. The test result will be reported as a percent of the counted events.
This test detects the antigen or spirochete in the blood. The antigen may not be present in the sample tested if there are very few spirochetes within the blood or if they are in some other organ of the body. Therefore, a negative test does not mean the patient does not have Lyme disease. It means that we were unable to detect Borrelia burgdorferi in the specimen.
The test was compared using our instrumentation and the instrumentation of our Flow Cytometer supplier. The test also was compared using Western Blot. However, this could not be used exclusively, as the Western Blot has only a 60% to 70% accuracy for Lyme disease. Data on 56 of the samples evaluated showed 97% of the tests that tested negative by Flow Cytometry were negative by Western Blot. 86% of the Western Blot positive tests were positive by Flow Cytometry. Only 29% of the tests positive by Flow Cytometry were positive by Western Blot. This was expected because the antibody may not be present in a person with Lyme disease.
Borrelia burgdorferi Direct Fluorescent Antibody by Flow Cytometry should be ordered with Wampole PreVue for best diagnostic results.
We feel this test will be a valuable aid for the physician in the diagnosis of Lyme and other diseases when used with symptoms and other tests for Borrelia burgdorferi.