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Post by freyman on May 31, 2008 23:54:10 GMT -5
Why is everyone so insistant that this organism is man-made when everything about it can be explained by nature. Every single detail that Dr Hildy uses as evidence in support of her claim can be explained by nature and in my opinion her stand on this issue does nothing but keep the mainstream from accepting it. Thats just my opinion but I stand by it.
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Post by skytroll on Jun 1, 2008 0:53:10 GMT -5
Because bottom up nano is made by nature, but trained by man in the man made blueprint, all appears natural because it is. The nano is natural, but what is being mixed, is a different recipe with different ingredients.
Like Archaea, like luciferin from the firefly. like lucerifase from the squid.
All is natural. of course, just rearranged.
new species. Man can manipulate nature, look at gm foods, all natural.
Skytroll
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Post by freyman on Jun 1, 2008 1:05:26 GMT -5
I personally do not believe man is involved in this in any way at all, other than being the host for this organism which I believe exhibits traits and characteristics that are consistent with organisms of the Phylums Porifera, and Cnidaria. I believe that this organism has infected literally every living thing on this planet over the course of hundreds of millions of years and has been infecting humans to the point where it may even be a part of our genome. As far as a coverup is concerned, I highly doubt it, the truth of this situation is just too bizarre for most people to accept, bizarre but not a result of man.
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Post by liatris on Jun 1, 2008 1:24:24 GMT -5
Hi Steve -
As I said in another thread, it really seems that not everyone on this board has the same thing so one would have to at least separate people into categorie..... I do not have fibers but these fibers are not burnable until they are exposed to 600-1400 degrees F. Could they be microbe made ? What about the metallic hexagons some people have ? They really do not seem "natural". Some people have all sorts of "bugs" - why decide that it is just one category of microbe ?
Hmm ... CDC must appreciate all this confusion amongst us - gives them more time to stall.
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Post by Sidney on Jun 1, 2008 2:15:54 GMT -5
I agree with Steve Frye that it isn't man made and all the other worldly talk seems to me to just muddy the waters and cloud the issues. (while the nay-sayers scoff and jeer)
I don't know WHAT it is, only that Collembola have been found and photographed by so many, along with Thrips and other garden variety critters. I found a carpet beetle embedded in my thigh probably twelve years ago. It was alive and had munched it's way right through my tissue. A friend in Florida found them embedded in her arms. Another friend in Pennsylvania fought them off as she sat at her computer in a room with no carpet.
I recently found three carpet beetles on the vanity top in my bathroom. I have NEVER found evidence of carpet beetles in this house, yet over a period of two weeks I found three of them crawling around on the vanity top, one covered with fibers I was collecting along with skin flakes and other debris from my skin that I'd placed on a glass laboratory slide.
Shortly after the carpet beetle trauma I felt something moving on my face and it was a Phorid Fly. Fruit flies, fungus gnats, all creepy crawlies seem to be attracted to me. WHY!?
Regarding the metallic hexagon things I thought "we" had established they were Diatoms.
What about all the black speckls? Some I've removed from my face look a lot like mouse poop only smaller, and usually have a thread or two entwined. Smaller black specks are often rectangular in shape or simply a tiny black speck with no particular shape.
Personally, I have never seen a fiber that wouldn't burn or melt.
Organic fibers burn easily, polyester fibers or fibers that contains polyester seem to melt easily. I have never understood the talk about fibers that won't burn. Even super thin wire, while it won't blaze, will soon yield to intense heat and can easily be broken.
Theories and beliefs are like Belly Buttons. Everyone has one. Some are innies and some are outies, but none are particularly attractive.
Reminds me of that old Sheb Wooley song "I don't look good naked anymore," or something along those lines.
These people aren't so bored, hungry for attention and eager to waste their time that they sit around and dream up ways to capture tiny wildlife and insist the wildlife came from their person.
What seems to be the most insurmountable task we face is convincing the world of medicine and science these garden variety or not so garden variety living creatures are not found ON our skin, but within our skin along with fibers, hair and other artifacts.
Then there are those who have no visible lesions, but have the biting, stinging and crawling sensations.
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Post by freyman on Jun 1, 2008 2:21:08 GMT -5
Hi Steve - As I said in another thread, it really seems that not everyone on this board has the same thing so one would have to at least separate people into categorie..... I do not have fibers but these fibers are not burnable until they are exposed to 600-1400 degrees F. Could they be microbe made ? What about the metallic hexagons some people have ? They really do not seem "natural". Some people have all sorts of "bugs" - why decide that it is just one category of microbe ? Hmm ... CDC must appreciate all this confusion amongst us - gives them more time to stall. The fibers, or spicules, do not burn at lower temperatures because of the silicon/silica which has a melting point above 2500 degrees F, the silicon is also responsible for the metallic appearance. en.wikipedia.org/wiki/Silicon
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Post by freyman on Jun 1, 2008 2:48:29 GMT -5
I agree with Steve Frye that it isn't man made and all the other worldly talk seems to me to just muddy the waters and cloud the issues. (while the nay-sayers scoff and jeer) I don't know WHAT it is, only that Collembola have been found and photographed by so many, along with Thrips and other garden variety critters. I found a carpet beetle embedded in my thigh probably twelve years ago. It was alive and had munched it's way right through my tissue. A friend in Florida found them embedded in her arms. Another friend in Pennsylvania fought them off as she sat at her computer in a room with no carpet. I recently found three carpet beetles on the vanity top in my bathroom. I have NEVER found evidence of carpet beetles in this house, yet over a period of two weeks I found three of them crawling around on the vanity top, one covered with fibers I was collecting along with skin flakes and other debris from my skin that I'd placed on a glass laboratory slide. Shortly after the carpet beetle trauma I felt something moving on my face and it was a Phorid Fly. Fruit flies, fungus gnats, all creepy crawlies seem to be attracted to me. WHY!? Regarding the metallic hexagon things I thought "we" had established they were Diatoms. What about all the black speckls? Some I've removed from my face look a lot like mouse poop only smaller, and usually have a thread or two entwined. Smaller black specks are often rectangular in shape or simply a tiny black speck with no particular shape. Personally, I have never seen a fiber that wouldn't burn or melt. Organic fibers burn easily, polyester fibers or fibers that contains polyester seem to melt easily. I have never understood the talk about fibers that won't burn. Even super thin wire, while it won't blaze, will soon yield to intense heat and can easily be broken. Theories and beliefs are like Belly Buttons. Everyone has one. Some are innies and some are outies, but none are particularly attractive. Reminds me of that old Sheb Wooley song "I don't look good naked anymore," or something along those lines. These people aren't so bored, hungry for attention and eager to waste their time that they sit around and dream up ways to capture tiny wildlife and insist the wildlife came from their person. What seems to be the most insurmountable task we face is convincing the world of medicine and science these garden variety or not so garden variety living creatures are not found ON our skin, but within our skin along with fibers, hair and other artifacts. Then there are those who have no visible lesions, but have the biting, stinging and crawling sensations. I believe that there is an explanation for all of it Sidney, if you just allow yourself to believe one factor that is not currently known to science, that the sponge is capable of copying and storing the genetic coding of other organisms which it has parasitized and that it can replicate these other organisms at will. This idea may sound very radical but there is considerable evidence to support it. Like the fact that organisms from 18 different Phylum's have been found living inside of sponge colonies, or the fact that the sponge possesses genetic coding for a nervous system when it has no resemblance of one itself, or the fact that the sponge genome contains genetic coding from all four kingdoms of life, Animals, Plants, bacteria, and Fungi, or the fact that the sponge, the simplest animal on the planet, has been found to have the largest genome of any animal, there simply is no other explanation for this.
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Post by skytroll on Jun 1, 2008 8:11:03 GMT -5
So, genetic engineering did not happen, evolution does not happen, there were no evo devo contructed novel organisms, there are no molecular machines Feynman was full of crap, there is no such thing as synthetic anything, everything is all natural.
Nature is as it always has been, Billions of dollars spent on the genome. For what? Why is the NIH involved with Physics, ge, gm foods, recombinant genes, which cannot do gene therapy, because the viruses used kill people?
bugs are the carrierers, there are other vectors,
Homebox genes can be used in plants, animals bacteria e-coli, what do we have the most of in our bodies? e-coli, Why did recombinant genes have to be made? They mimic natural life, you can't tell the difference.
Why all the money spent on molecular machines? on molucular studies?
Why all the new physics?
Why all the convergence, in bio/chem/physics?
Why all the relationship of genes, in nature to genes in us?
What is epigenetics for?
Do you know insect stem cells are used? in pesticides? do you know about freeze dried stem cells? human cells?
Get your head out of the deep freeze.
Do you know what nano is? or can't you even see it?
Same thing you are saying.
Nature can be manipulated, And Sydney yes, the sit in their Intellectual towers,
They have construed the natural theory of anything, stolen it and called it evolutionary theory, how much money spent on this?
Every university makes billions when one of their labs creates a new novel drug, a new novel organism, there is plenty of money in this.
The triplex; Govt/academia/Business
What is the difference between Eugenics and Epigenetics?
Nothing. One can cover for one by calling it pure human form, pure perfect human.
In this work, there is no God. Man is God.
When nature was tampered with, then we all got sick.
Very simple, but, their ideas from Star Trek, these people have not grown up.
And they have access to our genes.
If you can reduce everything, reductionism, down to the beginning cell, wouldn't you want to recreate? Seems that is what Evolutionary Theory, allowed by Guggenheim right from the beginning.
Protozoans/ sponges, how do we get them? Answer that!
Why are they in bugs? Why do bugs sense pheromones?
Some can only stay on one avenue, take a walk, ride around the block.
There is science out there, that is now incorporating other elements, elements not used before, and these people know the art, however, are they informing the public?
Well, investigative reporters can find the info, but, laziness is more important than digging for truth.
So, yes nature is involved, Nature magazine is fluff puff for the Evolutionary Giants.
We evolved, from what the sponge? the cnidarians, from the amoeba, from outer space? form particulate matter, from the ether? aether?
Why does Archaea need to be incorporated into the e-coli in our guts?
Building blocks of life, found everywhere.
Oh well, you accuse some of us of being in one box, so are you.
It is called "spontaneous generation" left out of Evo Devo theory.
Skytroll
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Post by ANTHILL on Jun 1, 2008 8:16:17 GMT -5
I agree with Steve Its been around a vary long time most folks have it--- why it affects some more than others just one of the many questions needing to be answered Probably a geranimal or geranimals that is not new to this world probably something that is cataloged in the annuls of biology but has a parasitic behavior that was missed in the cataloging process The only conspiracy that there seems to be in my eyes is the CYA (cover your a-s-s) policy that doctors adhere to when confronted with our problem
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Post by Jill on Jun 1, 2008 8:20:21 GMT -5
Agree with all you stated, Skytroll.
Jill
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Post by jj on Jun 1, 2008 8:42:28 GMT -5
From my perspective, man and the evolution of science and technology has done our health and well-being great harm. Mother nature provided. Man chose to manipulate and alter what was natural. New diseases and syndromes will continue to surface and man will continue to manipulate and alter.
JJ
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Post by godsgrace on Jun 1, 2008 9:20:45 GMT -5
The pathogenic Mycoplasma used to be very innocuous, but biological warfare research conducted between 1942 and the present time has resulted in the creation of more deadly and infectious forms of Mycoplasma. Researchers extracted this mycoplasma from the Brucella bacterium and actually reduced the disease to a crystalline form. They "weaponised" it and tested it on an unsuspecting public in North America.
Mycoplasma was originally found in nature. Thanks to Merck Pharma and US gov't, it was used in the creation of biological warfare. Now it is causing all kinds of illnesses that were not previously seen throughout the world.
How can you say that Morgellon's is natural in origin without considering that it too, may have been manipulated/rearranged?
kinda brassy to state something like that without all the evidence to back it up.
I have lost trust in the "rulers of the land" and I wouldn't put anything past them at this point.
best wishes
godsgrace
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Post by friskers on Jun 1, 2008 17:50:54 GMT -5
I agree with Steve Frye that it isn't man made and all the other worldly talk seems to me to just muddy the waters and cloud the issues. (while the nay-sayers scoff and jeer) I don't know WHAT it is, only that Collembola have been found and photographed by so many, along with Thrips and other garden variety critters. I found a carpet beetle embedded in my thigh probably twelve years ago. It was alive and had munched it's way right through my tissue. A friend in Florida found them embedded in her arms. Another friend in Pennsylvania fought them off as she sat at her computer in a room with no carpet. I recently found three carpet beetles on the vanity top in my bathroom. I have NEVER found evidence of carpet beetles in this house, yet over a period of two weeks I found three of them crawling around on the vanity top, one covered with fibers I was collecting along with skin flakes and other debris from my skin that I'd placed on a glass laboratory slide. Shortly after the carpet beetle trauma I felt something moving on my face and it was a Phorid Fly. Fruit flies, fungus gnats, all creepy crawlies seem to be attracted to me. WHY!? Regarding the metallic hexagon things I thought "we" had established they were Diatoms. What about all the black speckls? Some I've removed from my face look a lot like mouse poop only smaller, and usually have a thread or two entwined. Smaller black specks are often rectangular in shape or simply a tiny black speck with no particular shape. Personally, I have never seen a fiber that wouldn't burn or melt. Organic fibers burn easily, polyester fibers or fibers that contains polyester seem to melt easily. I have never understood the talk about fibers that won't burn. Even super thin wire, while it won't blaze, will soon yield to intense heat and can easily be broken. Theories and beliefs are like Belly Buttons. Everyone has one. Some are innies and some are outies, but none are particularly attractive. Reminds me of that old Sheb Wooley song "I don't look good naked anymore," or something along those lines. These people aren't so bored, hungry for attention and eager to waste their time that they sit around and dream up ways to capture tiny wildlife and insist the wildlife came from their person. What seems to be the most insurmountable task we face is convincing the world of medicine and science these garden variety or not so garden variety living creatures are not found ON our skin, but within our skin along with fibers, hair and other artifacts. Then there are those who have no visible lesions, but have the biting, stinging and crawling sensations. Sid I think you are probably pretty tasty ;D and luvable too Eat lots of raw garlic and onions and see what happens! maybe the critterswont like you anymore! They sure dont like me! On a side note I think belly buttons are pretty cute, especially the jeweled ones ;D
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doraisabel
Full Member
Have Faith in the LOrd, together we will get through this.
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Post by doraisabel on Jun 1, 2008 19:10:42 GMT -5
Steve,
If this is so natural and not manipulated in any way please explain to me why I had silver tears coming out of my eyes? What organism would cause me to have that? I also had black patches of hair appearing and dissapearing just like magic? In a matter of a few seconds!! Oh but how do you explain the hearts all over my body? I have white hearts, indented hearts on my forehead and red hearts on my legs? Oh and the newest additon is a new heart on the side of my neck that is perfectly etched out in a red pigmentation? Any explanantion? Because out of the 27 doctors I have seen not one diagnosed me as delusional but they really could not figure out what I had. I have had 1000's of diagnostic tests and not one could figure out what was causing all these symptoms including cotton coming out of my head from a lesion. Oh and I almost forgot under a black lght I have glowing pink hearts that are only visble under a black light? Please explain what organism causes this reaction.
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Post by angela on Jun 1, 2008 20:06:44 GMT -5
I have silver (looks melted) samples that came out of my skin and my eye mucous as well. I still believe this is natural. An auto-fluorescent/electromagnetic organism could do that....... en.wikipedia.org/wiki/Autofluorescence...Biological autofluorescence: "Cells contain molecules, which become fluorescent when excited by UV/Vis radiation of suitable wavelength. This fluorescence emission, arising from endogenous fluorophores, is an intrinsic property of cells and is called auto-fluorescence to be distinguished from fluorescent signals obtained by adding exogenous markers. The majority of cell auto-fluorescence originates from mitochondria and lysosomes. en.wikipedia.org/wiki/Electromagnetic_wave......Ørsted was not the first person to examine the relation between electricity and magnetism. In 1802 Gian Domenico Romagnosi, an Italian legal scholar, deflected a magnetic needle by electrostatic charges. He interpreted his observations as The Relation between electricity and magnetism. Actually, no galvanic current existed in the setup and hence no electromagnetism was present. An account of the discovery was published in 1802 in an Italian newspaper, but it was largely overlooked by the contemporary scientific community. This unification, which was observed by Michael Faraday, extended by James Clerk Maxwell, and partially reformulated by Oliver Heaviside and Heinrich Hertz, is one of the triumphs of 19th century physics. It had far-reaching consequences, one of which was the understanding of the nature of light. As it turns out, what is thought of as "light" is actually a propagating oscillatory disturbance in the electromagnetic field, i.e., an electromagnetic wave. Different frequencies of oscillation give rise to the different forms of electromagnetic radiation, from radio waves at the lowest frequencies, to visible light at intermediate frequencies, to gamma rays at the highest frequencies. en.wikipedia.org/wiki/Magnetismen.wikipedia.org/wiki/Ferromagnetismen.wikipedia.org/wiki/MagnetiteFe3O4 .....member of the spinel group. en.wikipedia.org/wiki/Iron_oxideen.wikipedia.org/wiki/Spinel.....class of minerals of general formulation XY2O4 which crystallize in the cubic (isometric) crystal system, with the oxide anions arranged in a cubic close-packed lattice and the cations X and Y occupying some or all of the octahedral and tetrahedral sites in the lattice ........ including magnesium, zinc, iron, manganese, aluminium, chromium, titanium, and silicon. en.wikipedia.org/wiki/SiliconIn the form of silica and silicates, silicon forms useful glasses, cements, and ceramics. It is also a constituent of silicones, a class-name for various synthetic plastic substances made of silicon, oxygen, carbon and hydrogen, often confused with silicon itself. Silicon is an essential element in biology, although only tiny traces of it appear to be required by animals. It is much more important to the metabolism of plants, particularly many grasses, and silicic acid (a type of silica) forms the basis of the striking array of protective shells of the microscopic diatoms. Silica occurs in minerals consisting of (practically) pure silicon dioxide in different crystalline forms. Sand, amethyst, agate, quartz, rock crystal, chalcedony, flint, jasper, and opal are some of the forms in which silicon dioxide appears. (They are known as "lithogenic", as opposed to "biogenic", silicas.) Silicon also occurs as silicates (various minerals containing silicon, oxygen and one or another metal), for example feldspar. These minerals occur in clay, sand and various types of rock such as granite and sandstone. Asbestos, feldspar, clay, hornblende, and mica are a few of the many silicate minerals. Silicon is a principal component of aerolites, which are a class of meteoroids, and also is a component of tektites, which are a natural form of glass. --------------------------------- Makes me wonder what they first thought of the Venus Fly Trap when it was first discovered. Of course there was no Nano back then.... a "demon from hell" maybe? They probably killed fireflies when they were first spotted. The ugly fish with the glowing fishing pole growing out its' head (now that's something) I'm sorry, but nature is INCREDIBLE! All by itself! ------------------------- What i still want to to know is WHEN they think man made this? Does anyone know a date?
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Post by freyman on Jun 1, 2008 20:09:52 GMT -5
Steve, If this is so natural and not manipulated in any way please explain to me why I had silver tears coming out of my eyes? What organism would cause me to have that? I also had black patches of hair appearing and dissapearing just like magic? In a matter of a few seconds!! Oh but how do you explain the hearts all over my body? I have white hearts, indented hearts on my forehead and red hearts on my legs? Oh and the newest additon is a new heart on the side of my neck that is perfectly etched out in a red pigmentation? Any explanantion? Because out of the 27 doctors I have seen not one diagnosed me as delusional but they really could not figure out what I had. I have had 1000's of diagnostic tests and not one could figure out what was causing all these symptoms including cotton coming out of my head from a lesion. Simply based upon the information you have provided doraisable I would venture to guess that the "silver tears" could be a result of the presence of silicone, which is produced by the sponge as well as other marine invertebrates. The patches of hair could be along the same lines as the fibers that the disease is noted for, which I believe are spines and spicules which science has found to be tremendously diverse and their morphology inducible, often prompted by a defensive nature. There has been significant study performed on sponge spicules and science admits they are still a mystery, this tiny, simplistic animal is much more complex than we could ever imagine and it's biomineralization abilities baffle science, we cannot even begin to understand the dynamics that take place in a sponge, this is why we are now studying it in such detail and virtually every study brings new surprises. Regarding the heart shapes, in only a matter of a couple of minutes i was able to find a species of sponge with this characteristic, Calyx podatypa and here is a quote describing it. 64.233.167.104/search?q=cache:WH7oUQnGIGUJ:dpc.uba.uva.nl/ctz/vol71/nr04/art07+sea+sponge+heart+shape&hl=en&ct=clnk&cd=40&gl=usTo Godsgrace, I have considered and researched other angles including mans manipulation of life and the field of "crystal tectonics" and I find more evidence to support an all natural theory. My position on this matter is based upon what I have learned through researching various marine invertebrates and not on other peoples opinions or ideas. I can support everything I claim with scientific facts and findings, which is more than most can say about their theories. I have searched for the cause of this disease by researching marine invertebrates and I have committed hundreds if not thousands of hours to this research. It is pretty obvious, based upon the worldwide map at MRF, that this organism originated in water and if you were to plot the locations of shipping ports on that map of morgellons cases, like I have done, you will see a specific correlation to morg cases and ship ports, which is what I believe caused this disease to spread worldwide. I think it would only be fair that before you all critisize me for my position you should learn for yourselves just how much the traits and characteristics of the sponge fit in this puzzle and also just how much we really don't know about this animal. The only part of my theory where I have to apply logical conjecture is my belief that the sponge is capable of copying and storing other organisms gentic code and their replication of these organisms, every other part of my theory is based completely on scientific facts and findings.
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Post by liatris on Jun 1, 2008 20:41:48 GMT -5
Steve -
I, for one, appreciate your responses. Your theory pointing towards sponges is not something I have looked into but I will. I do believe that numerous "uncataloged" parasites are (and have been) causing humans, who are susceptible, grief for as long as we have been here.
The highly respected Dr. Daniel Brooks has single-handedly scoured numerous vertebrates in the wild and has identified thousands of "new" parasites. "Modern science" has not had the correct stains, microscope condensers, etc to even see, never mind identify all these "bugs". Dr. Brooks is of the opinion that due to various environmental changes, parasite species jumping to humans is going to become even worse. The interest in developing techniques that would allow for the medical profession to start to do this searching for the potentially human-infesting parasites is not there. I feel that there are many different parasites having at humans.
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doraisabel
Full Member
Have Faith in the LOrd, together we will get through this.
Posts: 203
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Post by doraisabel on Jun 1, 2008 21:51:19 GMT -5
Steve,
First Of all I would not trust anything that comes from wikipedia because anyone can go on that website and write what they want.
SEcond of all, I saw one of the best if not the best toxicologists at UCLA and he said humans do not flouresce and he could not explain the hearts that flouresce on my body also the Calyx podatypa is found in areas I have never visisted..
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Post by freyman on Jun 1, 2008 22:35:14 GMT -5
Steve, First Of all I would not trust anything that comes from wikipedia because anyone can go on that website and write what they want. SEcond of all, I saw one of the best if not the best toxicologists at UCLA and he said humans do not flouresce and he could not explain the hearts that flouresce on my body also the Calyx podatypa is found in areas I have never visisted.. I agree about wikipedia, I used the reference because it was handy and I would never depend on it as a sole source, in fact I once swore I would never go there again but when it is the first find to come up, you know what I mean. Regarding Calyx podatypa, I only used that reference to demonstrate that it is not uncommon to find heart shaped organisms in the oceans and not to infer that this was the species infecting you. The fact that we have fluorescent material in our bodies, by itself, is a compelling arguement that a marine invertebrate is present, the corals are the most common organisms to exhibit fluorescent characteristics but other marine invertebrates do as well including sponges and, unles I'm mistaken, it's either fluorescent proteins or a bacteria which is the source of this fluorescence, and the sponge is loaded with both. This may sound as a blanket to cover all aspects of this disease as well as many, many more diseases, but it is important to understand that a major factor in my theory is the idea that the sponge has a command of genetic code, and that it can either place it's DNA in a place we haven't thought to look or possibly it has no DNA and what we find when we study the sponge is the DNA of other organisms, organisms that it has parasitized. DNA is a sequence of amino acids with proteins and science is aware of the command of proteins that the sponge possesses, so to suggest that the sponge can mimic it's host's DNA is not a stretch, particularly since we already know that mycoplasmas possess this ability and mycoplasmas are a common symbiont of the sponge, maybe they are but a product of the sponge, this is an area I am still actively researching. To get an idea of what I am referring to research the genome of the sponge and research the "associated organisms" of the sponge and while doing so temper what you find with the idea that science may not be on the right track, since they seem to think that everything they find in the sponge is evidence that we evolved from them, like the genetic code for a nervous system that is almost identical to ours, and is it a coincidence that we with morgellons experience many symptoms assoiciated with our nervous system, I don't think it is, I think what they are finding is the genetic code that the sponge has copied from humans.
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Post by skytroll on Jun 1, 2008 23:42:32 GMT -5
Spontaneous generation from an artificial cell. Home » Science & Nature » Biotech/Pharmaceuticals » Synthetic Biology Ads by Google Adult Stem Cell Treatment Using bone marrow stem cells from your own body! www.xcell-center.com/BoneMarrowStable Protein Expression Low cost, high quality proteins from mammalian cells. www.exprotein.comSingle Cell Analysis Even with Living Cells ! Use Latest Technology for Various Applications www.palm-microlaser.comArtificial Insemination Expert Infertility Treatment. Find a Specialist in Your Area Now! www.integramed.comLoad Cell The Top Industrial Resource. Find Load Cells Quickly. Loadcells.Industrial101.com Cell Biology Research, articles, info, reviews Free Biotech Research Newsletter www.FierceBioresearcher.comSynthetic Biology Building Artificial Cells © Dianne Rees Sep 17, 2007 To understand how a cell works, you have to take it apart and put it back together. That's the thinking behind synthetic biology. We now know how to identify genes, segments of DNA that code for proteins, and how to get copies of these genes to replicate in different organisms. For example, we can introduce a human gene encoding an antibody into a plant and we can even get a protein found in spider web silk to be expressed in the milk of cows. The ultimate dream of a synthetic biologist is to take different cloned genes, wrap them in a cellular membrane, and provide the basic elements for RNA and protein synthesis to create a self-replicating cell that’s never existed before. Why Make a Synthetic Cell? If we can already make transgenic animals and plants, why make a synthetic cell? Synthetic biologists believe that because synthetic cells will be precisely engineered to contain known genes that interact in known ways, the cells will behave in a more predictable fashion. Some other reasons: - If you can build a cell from scratch, you can understand how it works. - Synthetic cells may be used to produce chemicals without relying on harmful solvents or toxic processes; i.e., synthetic biology could lead to “green chemistry.” - Synthetic cells may be used to create alternative sources of energy. - Synthetic cells may be designed to produce large quantities of proteins to treat diseases. - Cells may be engineered to attack environmental contaminants, aiding in bioremediation efforts. Despite the logic of this and the basic tools of DNA cloning already in hand, synthetic biology is still in its infancy. Putting together the over one hundred genes it can take to create an artificial cell is not a trivial task! Creating a Biological Circuit Recently, scientists at Harvard Medical School, led by Dr. Pamela Silver, in conjunction with researchers at MIT, took an exciting step forward in this field. The scientists placed two genes, each coding a transcription factor (TF), into yeast cells. Transcription factors are proteins that regulate the expression of messenger RNAs. The code of a messenger RNA is read by the cellular machinery involved in protein synthesis (e.g., transfer RNAs and ribosomes), allowing for the assembly of amino acids into a protein product. The first TF gene was engineered to respond to galactose, a kind of sugar molecule, by producing a TF protein (TF1) that could bind to a second TF gene, which in turn would produce a second TF protein (TF2). The TF2 protein could also bind to the second TF gene to increase its own expression. In other words, the scientists created a molecular circuit with a positive feedback loop. When engineered yeast cells were exposed to galactose, the first gene switched on and the cells produced the TF1 protein. Expression of the TF1 protein caused the second TF gene to turn on and the TF2 protein was expressed. The TF2 protein bound to the second gene, as expected, causing the cells to make more TF2 protein. The scientists then took the galactose away and the first gene turned off. However, the second gene continued to express the TF2 protein. Although the yeast cells were no longer exposed to galactose, the cells had a molecular memory of their exposure because they continued to express TF2. Interestingly, the yeast cells passed on this memory to their descendants as they divided and produced more cells that had never been exposed to galactose. Although the engineered yeast cells were not completely artificial cells, this study demonstrates that it’s possible to design a transcription-based logic device. Precise control over the switches that turn genes on or off in a cell can allow us to control how cells react to environmental cues as well as to artificial stimuli. This lets us control the behavior of cells and create new biological tools. For more information about this study, see Ajo-Franklin, et al. Genes and Development, Volume 21, Issue 18: September 15, 2007. biotechpharmaceuticals.suite101.com/article.cfm/synthetic_biologyskytroll Skytroll
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