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Post by robertalouise26 on Jan 6, 2008 23:55:59 GMT -5
Dearest Betsy thank you soo much for that idea. I have already changed it in my papers that I am taking with me tomorrow. Saying that some friends of mine on my website have been researching our ailment and have come up with this and this. I know where you are coming from Betsy. It would be b ad if I just rushed in to her office and babbled away. She is a young very astute Doctor and I have to tread warily. My heart is pounding all ready!!!! i am not good at being diplomatic I just always seem to rush things out of my mouth as soon as the thought comes into my head I know that I have to present myself as a rational woman. Thank you sooo much for pointing that out to me. Much love and best wishes. Roberta. ps fingers crossed
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Post by freaky on Jan 7, 2008 2:43:22 GMT -5
Whatever is in my dogs eye matting looks like plastic. They are long thick rod like things that are straight. Sometimes they appear like crystal, but then there are brown to black ones. Om, a comment for plastic trivia. ;D...........freaky
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Post by robertalouise26 on Jan 7, 2008 3:26:07 GMT -5
Dear Grady as Betsy pointed out I should go softly softly on this one. I HAVE TO GET THE DOCTOR ON MY SIDE. It was she who took the swab from my ear and it came back as P A i am sick of trying to spell the thing and pyschologically I HATE THE B B B "thing" I get myself all churned up when going to the Doctor especially with this condition. But I think tht she is young and with it and on the mark and that SHt CAN HELP US. I have known the office manager at the medical centre for over 20 years her name is Allison and we are quite good friends. I told her this morning what a good Doctor I thought that this one was and she was very pleased to hear that and would pass that on to the Senior Doctors at the medical centre . She was astute enough to take the swab and then prescribe Derbac M and aqueous cream which have really really helped me. She did say last time I saw her that there was another ear drop that was much better than the Vosol she prescribed but it is very very expensive $30 and I just cann't afford that. I already owe the chemist $28 for some stuff i had to get for my bowel before I had the colonoscopy. All I seem to do is go to the Doctor and then go to the chemist. I know that it probably sounds stupid but I am sort of hoping that she might use me as a guinea pig trying out different medications to see which one will knock this thing out!!! I guess that, that is a wild dream. I am not just thinking of my self Grady if I manage to get some awnsers it might help all of us!!!!!! At least i have been diagnosed once so people on the board can go to their Doctor and ask to have a test for it cann't they? I hope my brain works tomorrow. I will do my very best to keep calm and cool and just lay the facts that I know are true. Like the horrible itching and the stuff seeping out of my ears, the horrible smell in my ears and when I put any ear drops in my right ear it comes down into my mouth which makes me think that I have a perforated right ear drum. I don't think that anything can be done for that it just rectifies itself in time I guess. Well our Sidney isn't very well and she is going to the Dotor tomorrow she has been in a lot of pain and I am sure that she would like some loving thoughts sent her way. I know that we all hope that she has a speedy recovery. Grady how much do you know about this thing? Can it envade the entire body?? I h onestly think that it does. for the first five years (I am in my 12th year) I had these things on my skin that looked almost black they didn't have feet or legs didn't look like an insect or bug, they were shiney like a bit of plastic that just stuck to my body. I showed them to a nurse at the hospital and she said well that isn't a bug it doesn't have any limbs and I said to her I KNOW THAT!!!! BUT THAT IS WHAT IS CAUSING MY ITCHING!!!!!!!!! OF COURSE SHE THOUGHT I WAS BATS. I didn't have a clue i just no that this thing made me itch and itch and itch!!!!!! the black specs are the same but then there are the worm like things that definetly can and do move every where on my body. Well I have rambled on too long. Get back to everyone tomorrow. Love and best wishes. Roberta.
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Post by robertalouise26 on Jan 7, 2008 4:02:29 GMT -5
Dearest Sandy please wipe your dogs' eyes with warm water and salt. A teaspoon of salt to a mug of warm water do this three or four times and day and they will come right. Please do it Sandy I worry about them Use cotton wool or tissues. How are you dear friend? i am off to the Doctor tomorrow about my ears and the itching and the leaking of puss. I am trying to go through out the night with out using any drops so that if the Doctor takes another swap it won't be contaminated by anything that I put in my ear. So will have to take an extra sleeping pill to get me through the night. Hope to catch up with you tomorrow. Much love to both of you. Robbie
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Post by robertalouise26 on Jan 7, 2008 4:14:56 GMT -5
Dear Grady how on earth can we avoid plastic especially if we are manufacturing it in our bodies. Every night about 6pm I start sneezing and sneezing just like having an allergy and for the life of me I cann't think what it is apart from the fact that I eat at that time, I only eat once a day. Can I be allergic to the food I eat because it has been wrapped or packed in plastic? ? Best Roberta.
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Post by grady on Jan 7, 2008 9:20:27 GMT -5
Good advice Betsy, don't use the word internet when talking with doctors, they hate that and will write everything you say off before even hearing you out.
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Post by ruth on Jan 7, 2008 11:33:35 GMT -5
hi grady, dr. schwartz said pseudomonas stenotrophomonas? years ago. i did a year and a half of bactrim (generic sulfur abx.)
i've been trying to connect nuclear waste...... converted... and dumped from planes since i am 60 miles upwind from hanford. ancient microbes are being used to bind with uranium to create beads within fibers that are less water soluble. an exchange of silicon can replace the uranium. they are bacteria also. nuclear waste disposal via chemtrails? (head pressure keeps me down)
look at tams map. europe and united states. so this could be connected to oil spills on the atlantic?
sounds right to me!! my friend and i have talked about how they recapture these (synthesized) microbes, or do they?
it would be fun to see a map of where these microbes have been released for oil cleanup (alaska- i am in wa. state, my native friend said something is wrong with the fish, here).
i wonder if these oil eaters are included in wastewater treatments?
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Post by skytroll on Jan 7, 2008 18:08:01 GMT -5
Went to my doc today, breathing issues, have bronchitis, but am coughing up white stuff which to me looks like silicon. So, am wonder if the silca that could be in the construction of this pseudomona aueringosa is in lungs, Dr. Staninger says in lungs, kidneys and skin?
So, this stuff coming out of the cyst I have was mentioned, said it was abrasion. RIGHT! well anyway they are giving me levaquin.
I coughed up big white form, and there were fibers in it. Did mention that.
This med is supposed to help skin too, so will let you know how it works.
I do believe this was created by DOE genomes to life for bioremediation, but most likey has other applications. The pseudomona aueringosa or putida. I was not diagnosed with that, yet.
But, that could be the microbial business around our noses, and our cartilages of ears. I had this very bad on ears, seems was eating flesh of ears.
I had read about the bioengineered microbe, that eats plastic. This could be one and the same grady, will look for that exact construction.
You are onto this, and I believe it is in waters, I think it is what is killing the phytoplankton, and the water fleas. I can bet it can sporalize too from the algae. This is why these microbes are so dangerous. If they are build bottom up, then most would be natural except for the plastic, which could be inside bugs that bite us. Collembolas go where fermenting is, and if they are going after plastics in the fermenting gargage or fermenting waters, then what says they will not be incorporated into soils and waters?
Will keep focused here on this. Incidently, this is the same and one of the first things our Tam Tam mentioned in the "Superbug" He believes is part of the construction. So, we have 2 maybe four linking this, Dr. Hildy, Dr. Korz? Bell Vaneeden (tam tam connection), Dr. Swartz? maybe? and what was the "plastic worm" anyone heard of this? Is that Dr. Harvey's find? oh no, his was the acetomycetes, which would be fungal and a type of worm. But, this could be in collembola, gnats, aphids, flies, and any kind of worm. They are all near where this biodegradation would take place in the wild.
Read something about radionucleiotides used, that would be radio nuclear. Wonder if the chemtrails are under UN demand to get rid of uranium and/or radiation, along with the excess ground up plastics is being sprayed in the air, under the assumption that it would dissipate and/or dissolve in the air by use of the microbe? Far fetched. there was a mandate from the UN a while ago to get rid of the excess nuclear products.
How much can a body tolerate? Anyway it is the plastic eating microbe we are after, right?
Will try to find the DOE/Genomes2Life project.
What would be the difference between nucleotides, oligonucleotides, polyonucleotides radionucleotides and Real DNA? Dendrimers and dendrons could be the unnatural catalyst that makes these work?
Skytroll
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Post by toni on Jan 7, 2008 18:27:11 GMT -5
Skytroll,
I'm sorry to hear you're not feeling well. Levaquin for bronchitis is what I've always been prescibed and it's (to me-a miracle drug) I do think alot of that med. There's only two antibiotics my system can handle and Levaquin is one, and Bactrim is the other. Ginger had me on Levaquin for 4 months straight (one every single day non stop) and I had no adverse effects. As long as you don't have any "adverse reactions" your 10 day course (is that what it is?) you should feel great in 3-4 days...but finish it all...(which I know you know that).
Take care.
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Post by skytroll on Jan 7, 2008 18:29:29 GMT -5
don't know if this posted before. Microbes eating styrofoam? The microbes, a special strain of the soil bacterium Pseudomonas putida, converted polystyrene foam ?commonly known as Styrofoam??into a biodegradable plastic, according to Kevin O’Connor, Ph.D., of University College Dublin, the study’s corresponding author. The study is among the first to investigate the possibility of converting a petroleum-based plastic waste into a reusable biodegradable form." mention of polystyrene? Dr. Hildy's report? Grady, is this what she mentioned over phone? "O’Connor and his colleagues from Ireland and Germany, utilized pyrolysis, a process that transforms materials by heating them in the absence of oxygen, to convert polystyrene ?the key component of many disposable products ?into styrene oil. The researchers then supplied this oil to P. putida, a bacterium that can feed on styrene, which converted the oil into a biodegradable plastic known as PHA (polyhydroxyalkanoates). The process might also be used to convert other types of discarded plastics into PHA, according to O’Connor. PHA has numerous uses in medicine and can be used to make plastic kitchenware, packaging film and other disposable items. The biodegradable plastic is resistant to hot liquids, greases and oils, and can have a long shelf life. But unlike polystyrene, it readily breaks down in soil, water, septic systems and backyard composts. Worldwide, more than 14 million metric tons of polystyrene are produced annually, according to the U.S. Environmental Protection Agency. Most of this ends up in landfills. Although polystyrene represents less than 1 percent of solid waste generated in the United States, at least 2.3 million tons of it is dumped in U.S. land '"/> link: tinyurl.com/2gddl2www.bio-medicine.org/biology-news/Microbes-convert-Styrofoam-3F-into-biodegradable-plastic-2261-1/ polyhydroxyalkanoates other types of microbes as well: Polyhydroxyalkanoates (PHAs) PHAs make up a class of polymers that are fully biodegradable. They are a family of polyesters with a wide array of physical properties that can range from stiff-brittle plastics to elastomers to rubbers. PHAs are naturally produced in numerous genera of bacteria, and have been amplified through bacterial fermentation. PHAs are mainly composed of R-(-)-3-hydroxyalkanoic acid monomers. There can be broadly subdivided into two groups: Short chain length PHAs - consist of 3 carbon - 5 carbon monomers (C3-C5) - produced by bacterium Alcaligenes eutrophus (plus others) Long chain length PHAs - consist of 6 carbon - 14 carbon monomers (C6-C14) - produced by Pseudomonas oleovorans (plus others) Each type of PHA generally consists of 1000-10000 monomers, but most are synthesized by short chain lengthed monomers. There are many different types of PHAs, distinctly characterized by chain length, type of functional group and degree of unsaturated bonds. A higher degree of unsaturation increases the rubber qualities of a polymer, and different functional groups change the physical and chemical properties of a polymer. Figure scanned from Kumar & Minocha, In Transgenic Plant Research; Harwoo Pub. www.routledge.com PHB [or P(3HB)] is the most common type of PHA produced and is an example of a short chain lengthed homopolymer produced by A. eutrophus. PHB has poor physical properties for commercial use. It is stiff, brittle and hard to process. This has led to an increased interest to produce heteropolymers with improved qualities. P(3HB-3HV) is an example of an improved heteropolymer. It is named Biopol, and was produced by Zeneca, but now owned by Monsanto. Compared to PHB, P(3HB-3HV) is less stiff, tougher, and easier to process, making it more suitable for commercial production. It is also water resistant and impermeable to oxygen, increasing its value. PHB is 100% biodegradable. Various bacteria and fungi degrade PHB to carbon dioxide and water through secreting enzymes. It can also be degraded through non-enzymatic hydrolosis. Degredation appears to be the fastest under conditions of high temperatures and mechanical disruption. An 80% loss of PHB has been degraded in 15 weeks. PHB is also biocompatible, meaning it is a metabolite normally present in blood. Researchers have amplified the production of PHAs for large scale production, to produce and commercialize biodegradable plastics. Originally bacterial fermentation was used, but PHA can only be produced on a small scale this way, and production costs are to expensive compared to production of synthetic plastics. Researchers have since tried large scale production through transgenic plants, but many problems have arised this way also. More research must be done before commercial production of PHAs will beable to outcompete or replace synthetic plastics. references: Imam, S., Greene, R. and Zaidi, B. 1999. Biopolymers, Utilizing Nature's Advanced Materials. American Chemical Society, Washington, DC. 111 p. Lindsey, Keith. 1998. Transgenic Plant Research. Harwood Academic Publishers. Amsterdam, The Netherlands. 201-219 pp. Poirier, Y., Nawrath, C. and Somerville, C. 1995. Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers, in bacteria and plants. Bio/Technology 13: 142-149. tinyurl.com/22b9rg www.usask.ca/agriculture/plantsci/classes/plsc416/projects_2002/immel/polyhydroxyalkanoates.html Skytroll
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Post by skytroll on Jan 7, 2008 18:31:17 GMT -5
Thank you Toni,
So worried about taking antibiotics, but this is only for 10 days. Can't seem to kick this bugger, but sounds like this might work, hubby has used it before and helped him.
Skytroll
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Post by skytroll on Jan 7, 2008 18:55:03 GMT -5
Alcaligenes eutrophus - 3 carbon - 5 carbon monomers (C3-C5) TAM TAM mentioned C3 Pseudomonas oleovorans - 6 carbon - 14 carbon monomers (C6-C14) So monomers?: monomers: Concept 1: Monomers and Polymers Each cell assembles its own polymers by joining appropriate monomers together with the production of water. Most biological molecules are very large and are built by assembling small molecules, or monomers, into long chains. The resulting molecules are called macromolecules, or polymers. A process of linking monomers, called dehydration condensation, involves the removal of two hydrogen atoms and one oxygen atom to form water. One way this might happen is diagrammed above, where several generic monomers are shown with -OH groups that could be used for linking. The animation illustrates the joining together of monomers by condensation (catalyzed by a polymerase enzyme) and the reverse process, in which added water results in hydrolysis (catalyzed by a hydrolase enzyme). www.phschool.com/science/biology_place/biocoach/bioprop/monomers.htmlNote the list here: Some of the genes located in CH34 megaplasmids are shown below in table 1 . Table 1. DNA fragment Homologous to--from: P(n) Fragment 01 Phosphoribosylformylglycineamide synthase--E.coli 5.7e-62 Fragment 02 Nickel & cobalt reistance protein--Alcaligenes denitrificans 5.3e-54 Fragment 03 Nitrogen fixation protein--Haemophilus influenzae 2.2e-45 Fragment 04 Glycine dehydrogenase--E.coli 4.0e-45 Fragment 05 Putative L-lactate permease--E.coli 2.2e-43 Fragment 06 Long-chain-fatty-acid-CoA--Archeoglobus fulgidus 1.7e-24 Fragment 07 Phosphate binding protein--Xanthomonas oryzae 7.1e-20 Fragment 08 Hydrogenase expression/formation protein HYPC--Rhizobium leguminosarum 3.7e-13 Fragment 09 TniBdelta1 transposon Tn21--Pseudomonas aeruginosa 1.7e-11 Fragment 10 Transposase tnpA protein--Pseudomonas putida 1.8e-11 Fragment 11 Aconitate hydratase--Bacillus subtilis 5.3e-11 Fragment 12 Anthranilate synthase component I--Aquifex aeolicus 5.4e-09 Fragment 13 MocB protein--Agrobacterium tumefaciens 6.7e-09 Fragment 14 Proline dehydrogenase--E.coli 1.9e-08 Fragment 15 Oligopeptide transport ATP-binding protein OppD.-E.coli 6.7e-07 P aeruginosa mentioned in these, so there are more than one used to degrade not only sytrofoam into plastic but plastic itself: All using COOH configurations: Images: Alcaligenes eutrophus tinyurl.com/389gm9images.google.com/images?client=firefox-a&rls=org.mozilla:en-US:official&channel=s&hl=en&q=Alcaligenes utrophus&um=1&ie=UTF-8&sa=N&tab=wi The other: Pseudomonas oleovorans "Pseudomonas oleovorans as a source of bioplastics : production and characterization of poly(3-hydroxyalkanoates) Preusting, Johannes Cornelis Gerardus Poly(3-hydroxyalkanoates) WAS) are biological polyesters which are accumulated by a wide range of bacteria under conditions of excess carbon source and limiting concentrations of an essential nutrient such as nitrogen. Poly(3-hydroxybutyrate) (PHB), the best known example of these biopolymers has been extensively studied since its discovery in 1926. Only recently, it has been considered as a possible biodegradable substitute for conventional nondegradable plastics. PHI3 has long been thought the only bacterial polyester, until in the early 1980's a PHB resembling polymer was discovered in Pseudomom oleovorans after growth on n-octane. The P. oleovorans PHA distinguished itself from PHB by having longer pendant groups attached to a similar backbone. Moreover, it was found that the length of the pendant group was dependent on the carbon source used to grow the organism. Thus, the PHA-synthesizing system of P. oleovorans can be used to produce a range of PHAs. To be commercially interesting in the future, this novel class of polymers has to satisfy two important conditions: 1. besides the expected biodegradable properties, the physical and mechanical properties of any of the PHAs must be promising, and 2. PHAs must be available in large quantities. In this respect, we have examined some physical properties of a number of PHAs isolated from P. oleovorans and studied the production of one of them (the 'octane-derived' p"..... dissertations.ub.rug.nl/faculties/science/1992/j.c.g.preusting/So others are used and in differing manners. But it all comes down to the PHA polyhydroxyalkanoates Skytroll
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Post by grady on Jan 7, 2008 18:58:22 GMT -5
PSEUDO-OUTBREAK OF PSEUDOMONAS PUTIDA IN A HOSPITAL OUTPATIENT CLINIC ORIGINATING FROM A CONTAMINATED COMMERCIAL ANTI-FOG SOLUTION - VANCOUVER, BRITISH COLUMBIA www.phac-aspc.gc.ca/publicat/ccdr-rmtc/00vol26/dr2621eb.htmlIntroductionIn July and August 2000, Pseudomonas putida was recovered from 10 specimens originating from a tertiary-care hospital's ear, nose, and throat (ENT) outpatient clinic. The specimens, consisting of sinus aspirations and biopsies, were taken from 10 patients with refractory, chronic sinusitis. Physicians in the hospital's medical microbiology laboratory first became aware of a potential infection control problem on 6 July 2000, when four specimens from the ENT clinic grew P. putida. Within a week, the same organism was recovered from two more specimens originating from the same outpatient clinic. An infection control investigation was promptly launched to determine a potential environmental reservoir, as well as to limit subsequent contamination of patients' sinuses and their specimens. MicrobiologyP. putida is a gram-negative, aerobic, fluorescent pseudomonad, which grows optimally at room temperature. Like other pseudomonads, P. putida thrives in moist environments. Its isolation from clinical specimens, especially non-sterile sites, is a unusual event and is considered to have uncertain pathogenic significance(1). In immunocompromised patients, P. putida has been found to cause septicemia and septic arthritis(2,3). Nosocomial transmission of P. putida has only been reported twice(4,5). Investigation A review of the patients implicated in the P. putida cluster revealed a common examination room in the outpatient clinic. A preliminary investigation by the hospital's infection control nurse identified multiple potential reservoirs including the clinic's endoscopes, enzymatic cleaning and glutaraldehyde solutions, suction apparatus, and tap water. Initial environmental cultures failed to grow P. putida. Nevertheless, a number of recommendations were made to staff in the ENT clinic in order to improve infection control practices and minimize the possibility of nosocomial transmission of the organism. Sporadic cases of P. putida colonization/infection continued to be identified in the microbiology laboratory, however, and a more thorough secondary environmental investigation was launched. High colony counts (> 108 CFU/L) of P. putida were recovered from the examination room's solution of StaKleer, a commercial anti-fog product used to prevent condensation from forming on mirrors and endoscopes. Unopened stock solutions of StaKleer were also found to be contaminated with the same organism. Molecular epidemiologic testing of the clinical and environmental isolates is ongoing. DiscussionCommercial anti-fog solutions, which are marketed primarily for use in dental offices, may be used in the hospital setting, especially in endoscopy suites where condensation on endoscope tips and mirrors may prevent adequate visualization of tissues. These products are not usually sterilized by the manufacturer. Moreover, the bottles containing anti-fog solution are not sealed by the manufacturer and bottle caps are not shrink-wrapped in order to prevent contamination or tampering. Contamination of large bottles of anti-fog solution may not be detected in the dental setting where clinical specimens are not routinely sent for microbiologic culture. Contaminated commercial anti-fog solutions or instruments that have come into contact with contaminated solutions pose an infection control hazard when introduced into normally sterile sites, such as sinuses. ReferencesKiska D, Gilligan P. Pseudomonas. In: Murray PR, Baron EJ, Pfaller MA et al., eds. Manual of clinical microbiology. Washington DC: ASM Press 1999:517-25. Anaissie E, Fainstein V, Miller E et al. Pseudomonas putida: newly recognized pathogen in patients with cancer. Amer J Med 1987;82:1191-94. Macfarlane L, Oppenhein BA, Lorrigan P. Septicaemia and septic arthritis due to Pseudomonas putida in a neutropenic patient. J Infect 1991;23:346-47. Taylor M, Keane CT, Falkiner FR. Pseudomonas putida in transfused blood. Lancet 1984;2:107. CDC. Reported contamination of heparin soduim with Pseudomonas putida. MMWR 1986;35:123-24. Source: M Romney, MD, Medical Microbiology Resident, C Sherlock, MB, BS, Infection Control Officer, G Stephens, MD, Medical Microbiologist, A Clarke, MB, ChB, Director, Division of Medical Microbiology, Providence Health Care, University of British Columbia, Vancouver, British Columbia.
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Post by skytroll on Jan 7, 2008 19:03:39 GMT -5
I believe this is related to "spider silk" used in chemtrails?...... Abstract Pseudomonas putida KT2442 could accumulate medium-chain-length poly(hydroxyalkanoate)s (PHA) consisting of 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, and 3-hydroxydodecanoate from a wide range of carbon sources. In this study, the PHA synthase pha operon (phaC1-phaZ-phaC2) was knocked out and the vgb gene encoding vitreoscilla hemoglobin protein (VHb), which could enhance oxygen uptake rate especially at low oxygen concentration, was integrated into the P. putida KT2442 genome to replace the deleted fragment. The resulting mutant P. putida KTOY01 or gene-replaced mutant KTOY02 was used as the host to study PHA synthase properties and PHA production. Different PHA polymerase (PhaC) genes, phaCRe from Rastonia eutropha H16, phaCAc from Aeromonas cavie, and phaC2Ps from Pseudomonas stutzeri 1317, were expressed in the mutant strains to test the PhaC enzyme substrate specificity. The result showed P. putida KTOY01 or KTOY02 could provide not only mcl PHA monomers but also 3-hydroxybutyrate from fatty acids, which may allow the production of copolyesters poly(3HB-co-mcl 3HA). Plasmid pCJY10 containing phaC2Ps, phbA, and phbB genes encoding PHA polymerase, -ketothiolase, and acetoacetyl-CoA reductase, respectively, were transformed into P. putida KTOY01 and KTOY02. Shake-flask culture showed P. putida KTOY01 or KTOY02 (pCJY10) could accumulate poly(3HB-co-mcl 3HA) from glucose. The above result showed pha operon knockout mutant of P. putida KT2442 was a very useful host of great potential not only for studying PhaC synthase, but also for microbial production of copolyesters poly(3HB-co-mcl 3HA), which is very difficult to obtain." www3.interscience.wiley.com/cgi-bin/abstract/114116440/ABSTRACTPhaC synthase: Production and characterization of polyhydroxyalkanoates in Pseudomonas aeruginosa ATCC 9027 from glucose, an unrelated carbon source Authors: Rojas-Rosas, Oscar; Villafaña-Rojas, Juan; López-Dellamary, Fernando A.; Nungaray-Arellano, Jesús; González-Reynoso, Orfil Source: Canadian Journal of Microbiology, Volume 53, Number 7, 1 July 2007 , pp. 840-851(12) Publisher: NRC Research Press --------------------------------------------------------- Abstract: The production and characterization of polyhydroxyalkanoic acids (PHAs) from glucose in Pseudomonas aeruginosa ATCC 9027 is described. We determined that the synthesis of PHAs was not due to a complete lack of nitrogen source, as previously reported for other microorganisms. The synthesis of PHAs was observed during exponential growth and it depended on the carbon/nitrogen ratio in the culture. More significantly, the specific PHA accumulation rate in this phase was higher than that observed in the storage phase. This phenomenon was a consequence of higher extracellular production rates of gluconate and 2-ketogluconate detected during the storage phase. Therefore, the production of those acids decreased the synthesis of PHAs in P.aeruginosa. The maximum percentage of PHA accumulation obtained was 10.8% of the cell dry matter when all the glucose was consumed. The monomer composition of this PHA consisted only of saturated 3-hydroxy fatty acids (octanoic, decanoic, and dodecanoic acids) as shown by gas chromatography- mass spectroscopy and nuclear magnetic resonance analyses, where 3-hydroxydecanoic acid was the main component because of the high affinity of its PhaC synthase for this monomer. The physical properties of this PHA were determined by differential scanning calorimetry and gel permeation chromatography. La production et la caractérisation d'acides polyhydroxyalcanoïques (PHA) à partir de glucose chez Pseudomonas aeruginosa ATCC 9027 est décrite dans cette étude. À partir des résultats, nous avons déterminé que la synthèse de PHA n'est pas due à une absence totale de source d'azote, tel que rapporté précédemment chez d'autres organismes. La synthèse de PHA est observée durant la phase exponentielle de croissance et elle dépend du rapport carbone/azote de la culture. De façon plus significative, le taux d'accumulation de PHA spécifique lors de cette phase est plus élevé que lors de la phase d'accumulation. Ce phénomène est la conséquence de la production extracellulaire plus élevée de gluconate et de 2-céto-gluconate détectée lors de la phase d'accumulation. Ainsi, la production de ces acides diminue la synthèse de PHA chez P. aeruginosa. Le pourcentage maximal d'accumulation de PHA obtenu était de 10,8% de la matière sèche cellulaire quand tout le glucose est consommé. La composition en monomère de ce PHA consiste uniquement en acides gras 3-hydroxy saturés (octanoïque, décanoïque, dodécanoïque) tel que démontré par des analyses en GC-MS et en RMN, l'acide 3-hydroxydécanoïque en étant la composante principale à cause de la forte affinité de la PhaC synthase pour ce monomère. Les propriétés physiques de ce PHA ont été déterminées par calorimétrie différentielle à balayage et par chromatographie sur gel perméable. " www.ingentaconnect.com/content/nrc/cjm/2007/00000053/00000007/art00004skytroll
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Post by skytroll on Jan 7, 2008 19:07:22 GMT -5
Grady,
sounds exactly like the flu like stuff people are getting. This is proof of it.
Many are dying of this, unknown diagnosis of many dying. This gets in back of throat.
Okay, so it is made from genes of all kinds of bacteria. But, carbon is related to it, hence carbon buckyballs, and the Sigma connection.
Keep on going GRADY............
Skytroll
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Post by felixwillford on Jan 7, 2008 19:14:15 GMT -5
Who wants to write Ms. Gerberding???CDC Director, Julie Louise Gerberding, MD, MPH has been leading the Centers for Disease Control and Prevention (CDC) and the Administrator of the Agency for Toxic Substances and Disease Registry (ATSDR) since July 2002. She also serves as a Clinical Professor of Medicine at Emory University and an Associate Professor of Medicine at the University of California at San Francisco Link below for more informaton on Julie Louise Gerberding, M.D., M.P.H. www.cdc.gov/about/leadership/director.htm
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Post by skytroll on Jan 7, 2008 19:16:28 GMT -5
Psuedomonads to Pseuodoplasmids: What is the differences? DNA interaction with synthetic DNA? Psuedomonads: Pseudomonas is a gram negative bacterium. Pseudomonas species are responsible for a number of infections, including hot tub rash and swimmer's ear (malignant otitis externa), and can also be found in people with cystic fibrosis and diabetes. The seriousness of the illness varies from minor skin infections to life-threatening. * 2 months ago Source(s): www.merck.com/mmhe/sec17/ch190/ch... Plasmids: Plasmid From Wikipedia, the free encyclopedia Jump to: navigation, search Figure 1: Illustration of a bacterium with plasmids enclosed showing chromosomal DNA and plasmids. Figure 1: Illustration of a bacterium with plasmids enclosed showing chromosomal DNA and plasmids. A plasmid is an extrachromosomal DNA molecule separate from the chromosomal DNA and capable of autonomous replication. In many cases, It is typically circular and double-stranded. It usually occurs naturally in bacteria, and is sometimes found in eukaryotic organisms (e.g., the 2-micrometre-ring in Saccharomyces cerevisiae). The size of plasmids varies from 1 to over 400 kilobase pairs (kbp). There may be one copy, for large plasmids, to hundreds of copies of the same plasmid in a single cell, or even thousands of copies, for certain artificial plasmids selected for high copy number (such as the pUC series of plasmids). Plasmids can be part of the mobilome, since they are often associated with conjugation, a mechanism of horizontal gene transfer. The term plasmid was first introduced by the American molecular biologist Joshua Lederberg in 1952.[1] en.wikipedia.org/wiki/Plasmidpseudoplasmids: jb.asm.org/cgi/reprint/139/2/432.pdfpseudoplasmodiums: Pseudoplasmodium - The haploid slug-like phase of cellular slime molds that gives rise to the fruiting body. www.sparknotes.com/biology/microorganisms/protista/terms/term_16.htmlGo,......Grady Go............... Skytroll
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Post by grady on Jan 7, 2008 19:16:45 GMT -5
BINGOStartling posts with our bug "Pseudomonas putida" found in Morgellons patients. Before you read these keep in mind that "Pseudomonas putida" is not highly effective at killing and replicating like "Pseudomonas aeruginosa" it's just putting us through heck and reacting with compounds, sometimes producing plastic, and all kinds of things. MORGELLONS PATIENTS ARE TURNING UP WITH OUR SUSPECT IN ALL KINDS OF TESTING Search for "Pseudomonas Putida" on this page www.thenmo.org/rwupda04.htm1) Individual red and blue Morgellons fibers were placed in bacterial media and cultured at body temperature. Isolates of those bacterial populations were grown on lab preparative media, blood agar, chocolate agar and a type of media that tends to support fungi better than bacteria. The bacteria were stained with various stains and observed both alive and dead. The bacteria were separated out into pure cultures (I think). PCR was performed and the amplified DNA was sent to a commercial sequencing lab to do the DNA sequencing. Two different bacterial species were identified. They were: a) Pseudomonas Putida and b) Corynebacterium efficiens. Does this identification mean anything? I do not know. Both of these can cause infections, especially in immunocompromised individuals. Both of these bacterial types are found in soil and can be found in skin.
I found many more references, I think I am going to have to group all of this information together and submit it to someone, with this new evidence that this bacteria can create plastics I think our bug has been hiding in plain site.
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Post by skytroll on Jan 7, 2008 19:20:09 GMT -5
You got it K Marie,
lets do it. Even Canada has recognized this, what the he..... if wrong with the leaders of CDC?
CHICKEN sh.....
I think a well constructed letter is in order. WE GOT THE PROOF, and with cross over research we can supply the da.... proof. Anyone looking with any knowledge of toxins, and constructed novel organisms and microbes knows all about this including Gerberding........
SKytroll
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Post by skytroll on Jan 7, 2008 19:23:11 GMT -5
Cornybacterium is related to diptheria which was prevalent in the 1500's.
We got this baby.........
There ya go Grady, there is pseudomona aeruginosa and the created Putida for eating plastics.
However, these both may be related.
Created by DOE Genomes2life.......
Is in the wild, in the environment.........
Skytroll
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