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Post by jeany on Jan 16, 2010 14:30:33 GMT -5
The ATCC found that 27 of 56 cell lines had G6PD type A variant.24 Further analysis revealed that several of these cell lines possessed some, but not all, HeLa markers. It was hypothesized that these variations could represent somatic cell hybridization between the original cell line and the contaminating HeLa cells.
Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiencywww.merck.com/mmpe/sec11/ch131/ch131h.htmlGlucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked enzymatic defect common in blacks that can result in hemolysis after acute illnesses or intake of oxidant drugs (including salicylates and sulfonamides). The only important defect in the hexose monophosphate shunt pathway is caused by G6PD deficiency. Over 100 mutant forms of the enzyme have been identified. Clinically, the most common form is the drug-sensitive variety. This X-linked disorder is fully expressed in males and homozygous females and is variably expressed in heterozygous females. en.wikipedia.org/wiki/ZygosityBlack males and in < 10% of black females in the US and in lower frequencies among people from the Mediterranean basin (eg, Italians, Greeks, Arabs, Sephardic Jews). ** how many people here have 'Mediterranean - Caucasian blood'?..I'm partly Italian..My son shows signs of this (jaundice)..diagnosed as Gilbert's disease..I'm wondering, if this is rather the cause? G6PD deficiency reduces energy available to maintain the integrity of the red cell membrane, which shortens RBC survival. Hemolysis occurs commonly after fever, acute viral and bacterial infections, and diabetic acidosis. Less commonly, hemolysis occurs after exposure to drugs or to other substances that produce peroxide and cause oxidation of Hb and RBC membranes. These drugs and substances include primaquine, salicylates, sulfonamides, nitrofurans, phenacetin, naphthalene, some vitamin K derivatives, dapsone, phenazopyridine, nalidixic acid , methylene blue, and, in some whites, fava beans. Chronic congenital hemolysis (without drug use) occurs in some whites. Because older cells are selectively destroyed in blacks, hemolysis is usually self-limited, affecting < 25% of RBC mass; in whites, the deficiency is more severe, and profound hemolysis may lead to hemoglobinuria and acute renal failure. fhp.osd.mil/factsheetDetail.jsp?fact=21Glucose-6-phosphate dehydrogenase (G6PD) is an important protein (enzyme) that helps blood cells use sugars to produce energy. Normal amounts of G6PD protect red blood cells from certain drugs or chemicals. Heinz bodies. Acute hemolysis from glucose-6-phosphatase dehydrogenase deficiency is linked to the development of Heinz bodies, which are composed of denatured hemoglobin. The severity of the reaction is dependent on the type of G6PD deficiency (Mediterranean deficiency-Caucasian (most severe) ; Blacks (usually mild to moderate). In people with this deficiency, exposure to certain drugs, foods, and chemicals can result in damage or destruction of red blood cells. Destruction of red blood cells can cause serious illness. * I'm asking, how many of those above mentioned drugs/chemicals are found in our food and water or are even used in pesticides? Based on Kammy's 'Hela Cell' research, I'm wondering if this is our 'inherited genetic disorder' we all share? Jeany |
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Post by jeany on Jan 16, 2010 14:44:09 GMT -5
Heinz Bodies.jpg) Coccoid inclusion bodies resulting from oxidative injury to and precipitation of haemoglobin, seen in the presence of certain abnormal haemoglobins and erythrocytes with enzyme deficiencies. www.mondofacto.com/facts/dictionary?Heinz+bodies*Kammy, have you seen something similar in the 'dishes'?..what about the 'donuts'? Jeany
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Post by jeany on Jan 16, 2010 14:51:48 GMT -5
I forgot to ask: How many of you know if you had high levels of Bilirubin in your blood after birth?..meaning severe Jaundice? en.wikipedia.org/wiki/BilirubinI did and both of my children did, especially my son showed strong reactions. The docs told me, that mostly heavy, big newborns react more than other babies. This is of course a normal reaction with newborns, but I'm wondering, IF we have this 'inherited genetic disorder', that's the reason we reacted stronger than others? en.wikipedia.org/wiki/Neonatal_jaundiceJeany |
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Post by jeany on Jan 16, 2010 15:13:20 GMT -5
en.wikipedia.org/wiki/Neonatal_jaundiceCauses of Pathological Jaundice of Neonates
Increased production Fetomaternal blood group incompatibility: Rh, ABO Hereditary spherocytosisen.wikipedia.org/wiki/Hereditary_spherocytosisHereditary spherocytosis is a genetically-transmitted (autosomal dominant) form of spherocytosis, an auto-hemolytic anemia characterized by the production of red blood cells that are sphere-shaped rather than donut-shaped, and therefore more prone to hemolysis. In longstanding cases and in patients who have taken iron supplementation or received numerous blood transfusions, iron overload may be a significant problem, being a potential cause of cardiomyopathy and liver disease. Measuring iron stores is therefore considered part of the diagnostic approach to hereditary spherocytosis.  en.wikipedia.org/wiki/SpherocytosisIt is caused by a molecular defect in one or more of the proteins of the red blood cell cytoskeleton, including, spectrin, ankyrin, Band 3, or Protein 4.2. Because the cell skeleton has a defect, the blood cell contracts to its most surface-tension efficient and least flexible configuration, a sphere. The sphere-shaped red blood cells are known as spherocytes. .... causing a cycle whereby the body destroys its own blood supply (auto-hemolysis). Furthermore, the detritus of the broken-down blood cells--bilirubin--accumulates in the gallbladder, and can cause pigmented gallstones or "sludge" to develop. * who has/had problems with liver and gall bladder?..gall stones?..My gall bladder had to be removed (1995) due to a huge amount of gall stones and sludge causing severe problems. Jeany
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Post by skytroll on Jan 16, 2010 16:13:57 GMT -5
Jeany, this was the beginning of this, I believe! genetic defect causing glucose-6-phosphate dehydrogenase deficiency (G6PD), which is characterised by reduced NADPH levels. ================== G6PD related to glycosylation: oxidative damage, due to aerosol operations, oxygen levels have been reduced, this is why is important to get oxygen tablets, Dr. H sells them, called Opaline, for those who have not had Morgellons for a long time, this will help. Others sometimes we can only take every other day. This is across the board, all people have this, some are doing well, exercise will increase the oxygen, will kill the microbes causing this. We have to combat the excessive calcium or biomineralization going on. This is why we have kidney stones, gallbladder, pancreas problems, we have to increase oxygen. We need to supplement our oxygen, Allicin C, OXY C< OR Opaline. Why did this happen? why is de hydrogenase happening? ======= A dehydrogenase (also called DHO in the literature) is an enzyme that oxidizes a substrate by transferring one or more hydrides (H−) to an acceptor, usually NAD+/NADP+ or a flavin coenzyme such as FAD or FMN. en.wikipedia.org/wiki/Dehydrogenase========================= hydrides were transferred into cells>>>>>>>> Hydrides ========== Hydride is the name given to the negative ion of hydrogen, H−. Practically, the term hydride has two distinct but overlapping meanings. To most chemists, the term hydride refers to (1) a hydrogen center that formally reacts as a hydrogen anion and (2) hydrogen ligands in metal complexes.[1] A more antiquated meaning of hydride refers to any compounds hydrogen forms with another elements, ranging over most of the periodic table, groups 1–16. .......Bonding Hydrides' bonds range from very covalent to very ionic as well as multi-centered bonds and metallic bonding. Hydrides can be components of discrete molecules, oligomers or polymers, ionic solids, chemisorped monolayers, bulk metals, and other materials. While hydrides traditionally react as Lewis bases or reducing agents. Some metal hydrides behave as hydrogen-atom donors and as acids........... ...."Hydride intermediates are key to understanding a variety of homogeneous and heterogeneous catalytic cycles as well as enzymatic activity. Hydroformylation catalysts and hydrogenase both involve hydride intermediates. The energy carrier NADH reacts as a hydride donor or hydride equivalent."........... en.wikipedia.org/wiki/Glucose-6-phosphate_dehydrogenase=================== chemical reaction: starts the cascade........... ================== Hydride Intermediates, are things that are substituted in to make another novel chemical reaction, works like directed evolution where something is inferred and then a product is created in the inferred model, not done before, is new, what Darwin talked about when he said for evolution to happen, intermediates have to be found or created, Intermediates are being created in biology, and chemistry. Since in dehydrogenase, hydride is involved, and is an intermediate, that means it was derived from some novel chemical or new chemical reaction, relating to enzymes. THERE IS ORGANIC AND inorganic chemistry. ======================= ......Hydroformylation catalysts and hydrogenase both involve hydride intermediates.......... hydorformylation catalysts and hydrogenase Abstract The supported Co/SiO2 and Co/active carbon (A.C.) catalysts were studied as heterogeneous catalysts in the liquid-phase hydroformylation of 1-hexene. The pore size of silica support significantly influenced the activity and selectivity of Co/SiO2 catalysts in hydroformylation of 1-hexene. The silica supported cobalt catalyst exhibited the best hydroformylation reaction performance at 403 K, and catalytic activity for this kind of catalyst increased with the increasing initial reaction pressure. The addition of small amount of noble metal significantly improved the activity and selectivity for Co/SiO2. The solvent effects for Co/SiO2 and Co/A.C. catalysts in the hydroformylation reaction of 1-hexene were investigated and alcoholic solvents promoted the oxygenate formation significantly. Keywords: Hydroformylation; 1-Hexene; Syngas; Noble metal; Silica; Active carbon tinyurl.com/yjlof6o============================== nobel metal, ....................silica .................1-Hexene..................... COBALT................ reacts to CA2+...................... This is new chemical compounds......................... Alchem....... =================== 1-Hexene: (¦wən ′hek′sēn) (organic chemistry) CH3(CH2)3HC:CH2 Colorless, olefinic hydrocarbon boiling at 64°C; soluble in alcohol, acetone, ether, and hydrocarbons, insoluble in water; used as a chemical intermediate and for resins, drugs, and insecticides. Also known as hexylene. www.answers.com/topic/1-hexene-organic-chemistry========================== .......... "We know that 1-hexene is a 6 carbon chain (hex=6). We also know that it is an alkene with a double bond between the 1st and 2nd Carbon. We also know that every carbon will make 4 bonds; either with another carbon or a hydrogen. Therefore: 6 Carbons 2+1+2+2+2+3= 12 Hydrogens C6H12" wiki.answers.com/Q/What_is_the_molecular_formula_for_1-hexene&src=ansTT=================== .."1-hexene is a higher olefin, or alkene, with a formula C6H12. 1-hexene is an alpha-olefin, meaning that the double bond is located at the alpha (primary) position, endowing the compound with higher reactivity and thus useful chemical properties. 1-hexene is an industrially significant linear alpha olefin." =================== ."1-hexene is a higher olefin, or alkene, with a formula C6H12. 1-hexene is an alpha-olefin, next post: skytroll |
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Post by skytroll on Jan 16, 2010 16:28:22 GMT -5
Synthesis Industrially, 1-hexene is commonly manufactured by two main routes: via full range processes using the oligomerization of ethene and by on-purpose technology. Another route to 1-hexene, used commercially on smaller scales, is the dehydration of alcohols. Prior to the 1970s 1-hexene was also manufactured by the thermal cracking of waxes. Linear internal hexenes were manufactured by chlorination/dehydrochlorination of linear paraffins. Ethene molecules that are combined together to produce linear alpha olefins of various chain lengths with an even number of carbon atoms use ethene oligomerization and result in a distribution or “full range” of alpha olefins. Shell uses the Shell Higher Olefins Process (SHOP-process) to produce a broad range of linear alpha olefins using olefin metathesis and ethene oligomerization. Linde and SABIC (Saudi Arabian Basic Industries) have developed the α-SABLIN technology using the oligomerization of ethene to produce 21 percent 1-hexene. CP Chemicals and Innovene also have full range processes. Typically, 1-hexene content ranges from about twenty percent distribution in the Ethyl (Innovene) process, whereas only twelve percent of distribution in the CP Chemicals and Idemitsu processes. The on-purpose route to 1-hexene using ethene trimerization was first brought on stream in Qatar in 2003 by Chevron Phillips. The Sasol process is also considered an on-purpose route to 1-hexene. Sasol commercially employs Fischer-Tropsch synthesis to make fuels from synthesis gas derived from coal. The synthesis recovers 1-hexene from the afformentioned fuel streams, where the initial 1-hexene concentration cut may be 60% in a narrow distillation, with the remainder being vinylidenes, linear and branched internal olefins, linear and branched paraffins, alcohols, aldehydes, carboxylic acids and aromatic compounds. Lummus Technology has recently developed a new on purpose route where 1-butene reacts with itself (“autometathesis” or “self-disproportionation”) to produce 1-hexene. A semi commercial unit has been in operation in Tianjin, China to demonstrate the Lummus technology. Applications Applications The primary use of 1-hexene is as a comonomer in production of polyethene. High density polyethene (HDPE) and linear low density polyethene (LLDPE) use approximately 2–4% and 8–10% of comonomers. Another significant use of 1-hexene is the production of linear aldehyde via hydroformylation ( oxo synthesis ) for later production of the short-chain fatty acid heptanoic acid. www.answers.com/topic/1-hexene================== above pertains to gas.......but...........that last sentence pertains to oxygen synthesis to synthesize means ". to form (a material or abstract entity) by combining parts or elements" in this case a material.......................... yep,. ETHANE from ether............nobel gases................now can we believe in self assembly from a gas?..................... fills in the extra cellular matrix........ between cells, in tubes, lack of oxygen, makes them grow............mimics......... this is where it gets difficult and I lose many of you...........but,.....there are chemical reactions that are taking place that are building these ........ The parasite cannot be identified because it is forming in us........and then causing growths..... will try to explain than we can see the parasites configuration proteins........................ a pattern is presented........... Proteins from different organisms were used..........and different nobel gases in this case ethane................ ether................. ========================== ether: Ether is a class of organic compounds that contain an ether group — an oxygen atom connected to two alkyl or aryl groups — of general formula R–O–R.[1] A typical example is the solvent and anesthetic diethyl ether, commonly referred to simply as "ether" (CH3-CH2-O-CH2-CH3). Ethers are common in organic chemistry and pervasive in biochemistry, as they are common linkages in carbohydrates and lignin. en.wikipedia.org/wiki/EtherIt is about time we got this out there................... --------------------- linkages in carbohydrates and lignin.......... Proteomics.............proteoglycans................. |
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Post by kammy on Jan 16, 2010 16:33:06 GMT -5
I'm glad I don't have a long string of letters behind my name... that gives me the permission to say what's on my mind without worry from peers that I'm being absolutely scientifically ridiculous. Well, I know I have more rhythm than the average person... I love soul music... I've said many times, "I feel black on the inside"... Ok, so the big secret about Morgellons is - we've inherited HeLa cells from Henrietta with the G6PD A band, and one drop of black blood makes us all black?... the faulty G6PD is what is at work in sickle cell... we have a white/black person's version of sickle cell anemia? AND... we all have HPV-18 on top of it?......... our inheritance? HeLa is everywhere... it's in the air?... they have to worry about it in the air in pristine labs?... I'm afraid to look to see how HeLa has been modified? I know it's in pesticides... I've already looked. Doesn't this make you want to scream?  |
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Post by skytroll on Jan 16, 2010 16:42:56 GMT -5
Folks, I am thinking out loud here,,,,,,but, until we find why the dehyrogenase in the glucose 6 is causing problems, I believe, we cannot find out why intercellular productions are going on in our bodies. ether carbohydrates and lignin focusing on the lignin....... WE CAN"T DIGEST THE FOOD! SYMPOSIUM-LIGNIN AND FORAGE DIGESTIBILITY How Do Lignin Composition, Structure, and Cross-Linking Affect Degradability? A Review of Cell Wall Model Studies John H. Grabber* U.S. Dairy Forage Research Center, USDA-ARS, 1925 Linden Drive West, Madison, WI 53706 * Corresponding author (jgrabber@wisc.edu) ABSTRACT An Overview of Cell... Formation and Characteristics of... Model Studies of Grass... Prospects for Developing a... REFERENCES Because of the complexity of plant cell wall biosynthesis, the mechanisms by which lignin restrict fiber degradation are poorly understood. Many aspects of grass cell wall lignification and degradation are successfully modeled by dehydrogenation polymer-cell wall (DHP-CW) complexes formed with primary walls of corn Zea mays L. This system was used to assess how variations in lignin composition, structure, and cross-linking influence the hydrolysis of cell walls by fungal enzymes. Altering the normal guaiacyl, syringyl, and p-hydroxyphenyl makeup of lignin did not influence cell wall degradability; each unit of lignin depressed cell wall degradability by two units. Plants with perturbed lignin biosynthesis often incorporate unusual precursors into lignin and one of these, coniferaldehyde, increased lignin hydrophobicity and further depressed degradability by up to 30%. In other studies, lignin formed by gradual "bulk" or rapid "end-wise" polymerization of monolignols had markedly different structures but similar effects on degradability. Reductions in cell wall cross-linking, via oxidative coupling of feruloylated xylans to lignin or nucleophilic addition of cell wall sugars to lignin quinone-methide intermediates, increased the initial hydrolysis of cell walls by up to 46% and the extent of hydrolysis by up to 28%. Overall, these studies suggest that reductions in lignin concentration, hydrophobicity, and cross-linking will improve the enzymatic hydrolysis and utilization of structural polysaccharides for nutritional and industrial purposes. In ongoing work, we are developing a DHP-CW system for dicots and are investigating how cross-linking and various acylated and unusual monolignols influence the formation of lignin and the degradation of cell walls by rumen microflora. Abbreviations: CAD, cinnamyl alcohol dehydrogenase • DHP, dehydrogenation polymer • DHP-CW, dehydrogenation polymer-cell wall • G, guaiacyl • H, p-hydroxyphenyl • NMR, nuclear magnetic resonance • PME, pectin methyl esterase • S, syringyl crop.scijournals.org/cgi/content/full/45/3/820======================== "hydrolysis of cell walls by fungal enzymes. Altering the normal guaiacyl, syringyl, and p-hydroxyphenyl makeup of lignin did not influence cell wall degradability; each unit of lignin depressed cell wall degradability by two units. Plants with perturbed lignin biosynthesis often incorporate unusual precursors into lignin and one of these, coniferaldehyde, increased lignin hydrophobicity and further depressed degradability by up to 30%. In other studies, lignin formed by gradual "bulk" or rapid "end-wise" polymerization of monolignols had markedly different structures but similar effects on degradability. Reductions in cell wall cross-linking, via oxidative coupling of feruloylated xylans to lignin or nucleophilic addition of cell wall sugars to lignin quinone-methide intermediates, ...." quinone methide intermediates................... dehydrogenation polymer-cell wall (DHP-CW) complexes formed with primary walls of corn Zea mays L. Corn was the first to have claimed the Mobile Genetic Element........ explain later, if I don't lose train of thought. skytroll |
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Post by jeany on Jan 16, 2010 16:49:37 GMT -5
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Post by jeany on Jan 16, 2010 17:07:42 GMT -5
Skytroll..this is very interesting and thanks for participating. I'll take a closer look.
Just for clarification: are you stating that Alchem Laboratories, Alchem Chemicals has something to do with our disease? That 'they' have created whatever in a lab that at the end is causing our disease?
Jeany
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Post by skytroll on Jan 16, 2010 17:32:11 GMT -5
I know those Hela cells were used, but, there is more to it than that. They used 3T3 cells also from labs contaminated with cancer cells. These were used in the horizontal gene transfer products...........and in their labs to make these gene products. The Hela cell is immortal.................. but, those were used in labs, to make vaccines............etc........... However, it takes more than that if cancers are viruses, to mutate into the human DNa. A mutation from a virus had to take place. What is the virus? These were used in crop science...............and insecticides............but, it is the virus that does the damage. What virus? Herpes virus: .................1959................but there is more.............the xmrv comes in here later................ ======================= The mention this: pocks on the chorioallantois ======= The highly vascular fetal membrane that consists of the fused chorion and allantois, found adjacent to the eggshell in reptiles and birds and constituting the placenta in most mammals. ========================= chorioallantois an extraembryonic structure formed by union of the chorion and allantois, which by means of vessels in the associated mesoderm serves in gas exchange; in many mammals, it forms the placenta. medical-dictionary.thefreedictionary.com/chorioallantoischorion,,,,,,,,,,,,, allantois: ======== The allantois, along with the amnion and chorion (other embryonic membranes), identify humans as amniotes, along with reptiles, dinosaurs, birds, and other mammals. Of the vertebrates, only Ichthyopsidas (fish and amphibians) lack this structure. ..."Function This sac-like structure is primarily involved in nutrition and excretion, and is webbed with blood vessels. The function of the allantois is to collect liquid waste from the embryo, as well as to exchange gases used by the embryo. [edit] In reptiles, birds, and monotremes The structure first evolved in reptiles and birds as a reservoir for nitrogenous waste, but also as a means for oxygenation of the embryo. Oxygen is absorbed by the allantois through the egg shell. The allantois functions similarly in monotremes, which are egg-laying mammals.' ............."The human allantois is an endodermal evagination of the developing hindgut which becomes surrounded by the mesodermal connecting stalk. The connecting stalk forms the umbilical vasculature. These endodermal and mesodermal tissues together form the human umbilical cord. The allantois later definitively becomes the urachus, which removes nitrogenous waste from the fetal bladder[2].'........... ....."Pathology A patent allantois can result in urachal cyst." URACHAL CYST: "Urachal cyst From Wikipedia, the free encyclopedia Jump to: navigation, search Urachal cyst Classification and external resources ICD-10 Q64.4 ICD-9 753.7 DiseasesDB 32765 MeSH D014496 A urachal cyst is a sinus remaining from the allantois during embryogenesis. It is a cyst which occurs in the remnants between the umbilicus and bladder.[1] This is a type of cyst occurring in a persistent portion of the urachus, presenting as an extraperitoneal mass in the umbilical region. It is characterized by abdominal pain, and fever if infected. It may rupture, leading to peritonitis, or it may drain through the umbilicus.[2] Urachal cysts are usually silent clinically until infection, calculi or adenocarcinoma develop.[3]" calculi, SAID THIS IS WHAT WAS IN MY SON'S PANCREATIC TUBE: CALCULINI: CALCULI: STONE FORMATION..........KIDNEY, GALL BLADDER, PANCREAS ETC. Pancreatic Stone Protein of Pancreatic Calculi in Chronic Calcified Pancreatitis in Man ....."pancreatolithiasis, persistent stasis of protein-rich pancreatic juice secondary to partial obstruction in the pancreatic duct leads to the calculus formation [1, 2]." PANACREATOLITHIASIS: DESCRBED AS: pancreatolithiasis the presence of calculi in the ductal system or parenchyma of the pancreas." "Through the analysis of pancreatic juice, the iron-binding protein, lactoferrin, has been found to be secreted in greater amounts by patients with chronic pancreatitis [3, 4, 5, 6, 7]. Lactoferrin may play a role in the formation of the protein plugs frequently seen in chronic pancreatitis because of its ability to produce an aggregation of a large acidophilic protein, such as albumin [8]. Pancreatic stone protein (PSP) is a 16 kDa acidic protein with an isoelectric point in the range of pH 5.5-6. A truncated form of this protein was originally isolated from calcium carbonate stones surgically removed from the main pancreatic duct of humans with chronic pancreatitis [9]. PSP was believed to serve as an inhibitor of calcium carbonate precipitation in pancreatic juice and was called "lithostathine" by some [10]. Increasing numbers of studies suggest that PSP has no more crystal inhibitory activity than endogenous proteins such as serum albumin and trypsinogen [11, 12]. PSP is highly susceptible to trypsin cleavage at its ARG11-ILE12 bond [13]."j ........."gold particles is diffuse from the center to the periphery of the stones.".......... www.joplink.net/prev/200203/04.htmlplease see picture of the gold particles........................ ========================= golden head?  ?or heads? that is just the pancreas, many are having gall bladder problems and kidney problems. more here snippets::::::::::: "In the present study, a significant correlation was obtained between total protein and pancreatic stone protein when patient A5 was excluded because the pancreatic stone was composed predominantly of fatty acid calcium and not of calcium carbonate. A wide range of percentages of pancreatic stone protein in the total protein (ranging from 0.01 to 41.9 %) suggests that the mechanisms and protein components involved in the stone formation are multifactorial, and that pancreatic stone protein is not the sole protein involved in stone formation [3, 4, 5, 6, 7, 13, 15, 19, 25]." ...."trypsinogen, amylase and pancreatic stone protein S1 " ================================== I was here once before..................this time we will get it............... CaCo3 particles..................... ======================== At the optical level the sludge presented as an organic matrix with embedded small quantities of CaCO3 crystals (and in one case CaCO3 microcalculi). Electron microscopy showed the presence of bacterial ghosts and protein threads. The characteristic pattern of proteolysed pancreatic proteins was obtained when the organic matrix was analysed by SDS-Page. The presence of trypsinogen, amylase and one of the molecular secretory forms of Pancreatic Stone Protein (PSP) was confirmed by Western-blotting. PSP was also found in association with CaCO3 crystals by immunolocalization. These results suggest that endoprosthesis clogging is due to the precipitation of whole pancreatic juice protein, probably triggered by uncontrolled proenzyme activation." endogenous proteins such as serum albumin and trypsinogen trypsinogen: serum albumin: gas exchange? ?? mic.sgmjournals.org/cgi/reprint/20/1/105.pdfHoly crapppppppppppppppppppppppp......closer ...........closer............to the protozoan...... www.jstor.org/pss/3225990Euplotes skytroll |
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Post by jeany on Jan 16, 2010 17:34:41 GMT -5
en.wikipedia.org/wiki/Hereditary_spherocytosisIn longstanding cases and in patients who have taken iron supplementation or received numerous blood transfusions, iron overload may be a significant problem, being a potential cause of cardiomyopathy and liver disease. Measuring iron stores is therefore considered part of the diagnostic approach to hereditary spherocytosis.
Iron overloadHistorically, the term haemochromatosis was initially used to refer to what is now more specifically called haemochromatosis type 1 (or HFE-related hereditary haemochromatosis). Currently, haemochromatosis (without further specification) is mostly defined as iron overload with a hereditary/primary cause or originating from a metabolic disorder. However, the term is currently also used more broadly to refer to any form of iron overload, thus requiring specification of the cause, for example hereditary haemochromatosis. The term haemosiderosis is generally used to indicate the pathological effect of iron accumulation in any given organ, which mainly occurs in the form of haemosiderin
Haemochromatosis (iron over load)...Organs commonly affected by haemochromatosis are the liver, heart and endocrine glands. The causes can be distinguished between primary cases (hereditary or genetically determined) and less frequent secondary cases (acquired during life). Jeany
HFE hereditary hemochromatosisHemochromatosis type 1(or HFE hereditary hemochromatosis, or HFE-related hereditary haemochromatosis) is a hereditary disease characterized by excessive absorption of dietary iron resulting in a pathological increase in total body iron stores. Humans, like most animals, have no means to excrete excess iron. Excess iron accumulates in tissues and organs disrupting their normal function. The most susceptible organs include the liver, adrenal glands, the heart and the pancreas; patients can present with cirrhosis, adrenal insufficiency, heart failure or diabetes. The term "hemochromatosis" has also been in contexts where there had not been a known genetic association for the iron accumulation. However, it should be noted that in some cases, the understanding of a condition that was considered due to behavior can be refined to accommodate new known genetic associations, as in African iron overload...HeLa Cells..from Afro-American woman.. en.wikipedia.org/wiki/African_iron_overloadHaemochromatosis is protean in its manifestations, i.e., often presenting with signs or symptoms suggestive of other diagnoses that affect specific organ systems. Many of the signs and symptoms below are uncommon and most patients with the hereditary form of haemochromatosis do not show any overt signs of disease nor do they suffer premature morbidity.An increased susceptibility to certain infectious diseases caused by siderophilic microorganisms:
Vibrio vulnificus infections from eating seafood or wound infection Listeria monocytogenes Yersinia enterocolica Salmonella enterica (serotype Typhymurium) Klebsiella pneumoniae Escherichia coli Rhizopus arrhizus
Mucor species--------------------------------------------------------------------- Rhizopus arrhizus  en.wikipedia.org/wiki/Rhizopus_arrhizusMucor en.wikipedia.org/wiki/MucorMucor species:  Yersinia enterocolitica en.wikipedia.org/wiki/Yersinia_enterocoliticaYersinia enterocolitica is a speices of gram-negative coccobacillus-shaped bacterium, belonging to the family Enterobacteriaceae....remember Kammy? Primarily a zoonotic disease (cattle, deer, pigs, and birds), animals that recover frequently become asymptomatic carriers of the disease. Vibrio vulnificus en.wikipedia.org/wiki/Vibrio_vulnificusVibrio vulnificus causes an infection often incurred after eating seafood, especially oysters; the bacteria can also enter the body through open wounds when swimming or wading in infected waters, or via puncture wounds from the spines of fish such as tilapia. Symptoms include vomiting, diarrhea, abdominal pain, and a blistering dermatitis that is sometimes mistaken for pemphigus or pemphigoid. Health officials clearly identified strains of V. vulnificus infections among evacuees from New Orleans due to the flooding there caused by Hurricane Katrina.--------------------------------------------------------------------- Males are usually diagnosed after their forties and fifties, and women several decades later, owing to regular iron loss through menstruation (which ceases in menopause). The severity of clinical disease in the hereditary form varies considerably. There is evidence suggesting that hereditary haemochromatosis patients affected with other liver ailments such as hepatitis or alcoholic liver disease suffer worse liver disease than those with either condition alone. There are also juvenile forms of hereditary haemochromatosis that present in childhood with the same consequences of iron overload. Jeany |
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Post by skytroll on Jan 16, 2010 17:39:22 GMT -5
Yes I am . was lab created.................
Alchemy is being used, however, it does have a magic component to it as well.
Alchemy is nano, top down and bottom up. zero point is when they combine.
Where two are gathered, there always is one , who may not agree.
the ying yang, the good/bad, but when the snake eats its own tail, it becomes
circular.............round and round we go.................
do you know about the superbug? and that gene products that were made were used in plants. heat shock proteins from archaea? those archaea
belong in volcanoes.
Recreating the cell...........first biologically, then artificially......
Won't be at the bioweapons labs, they just passed it along
to the crop scientists and DOE. Well hidden in
universities now. the Triangle.
Universities, government, Pharma (businesses)......
don't forget the foundations, have a say in science in the
lab itself..........................
Seems funding is now being lowered for the biotech industries,
that is the government funded.
skytroll
skytroll
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Post by jeany on Jan 16, 2010 17:43:03 GMT -5
Great info, Sky.
'"Through the analysis of pancreatic juice, the iron-binding protein, lactoferrin, has been found to be secreted in greater amounts by patients with chronic pancreatitis.
Do you think that the Pancreas is secreting more lactoferrin due to the iron over load associated with G6PD?
Jeany
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Post by skytroll on Jan 16, 2010 17:56:35 GMT -5
Jeany, I think that is possible, but there is a protozoan that has calculi. euplotes: here are some images of them: tinyurl.com/ybk4s59from droplets?................. Lactose intolerance precedes the pancreas problems. There is a lactose operon: ====== this is 1984" New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition Abstract We describe below several new variants of the tetracycline-resistance transposon Tn10 which are more useful than the wild-type transposon for many types of genetic and physical analysis of bacteria. These derivatives have one or more of the following new properties: (i) new drug resistance markers; (ii) high transposition frequencies; (iii) removal of the transposase gene to a position outside of the transposing segment; (iv) internal deletions which eliminate the ability of Tn10 to make adjacent deletion/inversions; or (v) addition of a trp-lac operon fusion segment just inside one terminus such that insertion can automatically generate a transcriptional fusion to the interrupted operon. Phage and plasmid vehicles carrying these new elements are described. Keywords: Tn10; transposon; insertion mutagenesis; lacZ fusions vectors; IS elements Abbreviations: bp, base pairs; IPTG, isopropyl-β-d-thiogalactopyranoside; IS, insertion sequence; kb, 1000 bp; λym broth, see PROCEDURES, section d4; moi, multiplicity of infection; ptac, tac promoter R; R, resistance tinyurl.com/y8gb6r9skytroll |
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Post by kammy on Jan 16, 2010 18:50:06 GMT -5
I know those Hela cells were used, but, there is more to it than that. They used 3T3 cells also from labs contaminated with cancer cells. These were used in the horizontal gene transfer products...........and in their labs to make these gene products. The Hela cell is immortal.................. but, those were used in labs, to make vaccines............etc........... However, it takes more than that if cancers are viruses, to mutate into the human DNa. A mutation from a virus had to take place. What is the virus? These were used in crop science...............and insecticides............but, it is the virus that does the damage. What virus? Herpes virus: .................1959................but there is more.............the xmrv comes in here later................ ======================= The mention this: pocks on the chorioallantois ======= Hi Sky, ty for your input here, we appreciate it. Yes! That's what we're seeing - the Morgellons sphere is what is called an "Immortal Cell" - it has to be, from all the different places we're seeing it. And, it has to have been around a while. We're seeing it cultured in certain tap water, paper products, paper products believed to be from China and their waters, cotton products also that are believed to be from the waters of China, tobacco paper, tobacco filters, certain red wines, a part of a GM insect's body functions, our blood, lesions, lesion debris, etc. So, T3T is an "Immortal Cell" also, like HeLa? T3T www.bioinfo.org.cn/book/Great%20Experments/great22.htm"We began cultivation of mouse embryo fibroblasts with a view to establishing an immortalized cell line suitable as a target for viral transformation. At that time, it was believed that mammalian cells became immortalized in culture only rarely and that it was impossible to predict when such an event might occur or under what conditions. In order to avoid haphazard conditions of cultivation, I thought it necessary to keep both the inoculation density and the transfer interval constant during repeated subcultivation, because those two variables might influence the ability of the cells to become immortalized; in addition, knowledge of the correct conditions might make it possible to develop immortalized cell lines reproducibly." www.olympusfluoview.com/gallery/cells/3t3/3t3cells.html"Embryonic Albino Swiss Mouse Fibroblast Cells (3T3 Line) Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse (Mus musculus) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. Variants of the initial cell line have been tested and found negative for ectromelia virus (mousepox), but most are susceptible to polyoma and simian virus 40 (SV40)."  T3T**Yes, I have several photos that show our sphere in a web network like this shown above. |
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Post by jeany on Jan 16, 2010 18:54:50 GMT -5
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Post by kammy on Jan 16, 2010 19:15:23 GMT -5
Polyomavirus en.wikipedia.org/wiki/Polyomavirus"Subsequently, many polyomaviruses have been found to infect birds and mammals. Polyomaviruses have been extensively studied as tumor viruses in humans and animals, leading to fundamental insights into carcinogenesis, DNA replication and protein processing. The tumor suppressor molecule p53 was discovered, for example, as a cellular protein bound by the major oncoprotein (cancer-causing protein) T antigen made by Simian vacuolating virus 40 (SV40). The avian polyomavirus sometimes referred to as the Budgerigar fledgling disease virus is a frequent cause of death among caged birds. They are potentially oncogenic (tumor-causing); they often persist as latent infections in a host without causing disease, but may produce tumors in a host of a different species, or a host with an ineffective immune system." SV40 en.wikipedia.org/wiki/SV40"SV40 is an abbreviation for Simian vacuolating virus 40 or Simian virus 40, a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors, but most often persists as a latent infection. The virus was first identified in 1960 in cultures of rhesus monkey kidney cells that were being used to produce polio vaccine. " Papillomavirus en.wikipedia.org/wiki/PapillomavirusInteresting article about HPV-18 www.inspire.com/groups/national-cervical-cancer-coalition/journal/interesting-article-about-hpv-18/"HPV-18 is the only strain of the papillomavirus that the Chow-Broker lab has reproduced using the new method, but they say it should work on other HPV types. There are at least 120 different strains of HPV. While most are relatively harmless skin viruses, there are some, like HPV-18, that are linked to genital warts, dysplasias, and cervical and penile cancers and certain oral cancers. Such lesions can be identified using Pap smear cytology or new more sensitive and accurate molecular tests. "HPV is part of the human condition," Broker said. "Virtually every adult in the world has been infected with HPV at some point in their life. Many of us will have no ill effects. Nonetheless nearly 1,000 women die each day from cervical cancer worldwide." |
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Post by jeany on Jan 16, 2010 19:26:00 GMT -5
Secondary haemochromatosis..Iron over load Multiple frequent blood transfusions (either whole blood or just red blood cells), which are usually needed either by individuals with hereditary anaemias (such as beta-thalassemia major, sickle cell anaemia, and Diamond-Blackfan anaemia) or by older patients with severe acquired anaemias such as in myelodysplastic syndromes. en.wikipedia.org/wiki/Sickle_cell_anaemiaHemolytic crises are acute accelerated drops in hemoglobin level. The red blood cells break down at a faster rate. This is particularly common in patients with co-existent G6PD deficiency.Cholelithiasis (gallstones) and cholecystitis, which may result from excessive bilirubin production and precipitation due to prolonged haemolysis. Decreased immune reactions due to hyposplenism (malfunctioning of the spleen). Leg ulcers Chronic pain: Even in the absence of acute vaso-occlusive pain, many patients have chronic pain that is not reported.
An acute sickle-cell crisis is often precipitated by infection. Therefore, a urinalysis to detect an occult urinary tract infection, and chest X-ray to look for occult pneumonia should be routinely performed. Sickle-cell anaemia is caused by a point mutation in the β-globin chain of haemoglobin, causing the hydrophilic amino acid glutamic acid to be replaced with the hydrophobic amino acid valine at the sixth position. Jeany
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Post by skytroll on Jan 16, 2010 19:26:27 GMT -5
: Tn10; transposon; insertion mutagenesis; lacZ fusions vectors; IS elements lets DIGEST each of these. This is how we get to the core............You all are great. but, to see it ........is so hard to explain. ..........CHAOS into order........and it is there. ================= Tn10: Easy cloning of mini-Tn10 insertions from the Bacillus subtilis chromosome. ABSTRACT Delivery vectors for mini-Tn10 transposons function in Bacillus subtilis (M. A. Petit, C. Bruand, L. Janniére, and S. D. Ehrlich, J. Bacteriol. 172:6736-6740, 1990). Using this system, we identified a new gene (sytA) whose inactivation affected regulation of genes of sucrose metabolism. For cloning the sytA::Tn10 insertion in Escherichia coli, we developed a methodology similar to that commonly used for B. subtilis Tn917 insertions. We constructed a plasmid which can be used to insert (by in vivo recombination) a ColE1 origin linked to a spectinomycin resistance gene (ori-spc element) into mini-Tn10 transposons inserted into the B. subtilis chromosome. DNA extracted from a sytA::Tn10::ori-spc transformant was cut with restriction enzymes that do not cut into the Tn10::ori-spc sequence; plasmids containing the sytA::Tn10 insertion were cloned by self-ligation, followed by transformation of E. coli. To obtain the wild-type sytA region, one of these plasmids was ligated with an E. coli-B. subtilis shuttle vector conferring erythromycin resistance, and the hybrid was used to transform the wild-type B. subtilis strain. Erythromycin-resistant transformants, detected as spectinomycin sensitive, resulted from conversion of the insertion mutation by the resident wild-type locus. The shuttle plasmid containing the wild-type locus could then be recovered in E. coli. jb.asm.org/cgi/content/abstract/176/6/1761================================= our strands are dna and chromosomes.............................. looks like the vector is b. subtilis, carries the Tn10.................... remember the spectin? E=Spectin?//////////// The walking DNA..............and I know where it went from there......... HEre is the strand, the clear hollow fiber stand................ the beginning.......... It is a new chromosome inserted into humans.............period................. ================== Jjill, remember the 12 stands and the e-coli as the basis of holding the genes? it was in the nucleic acids................ the nucleosome............. the submicron particle................... we will all get there in our separate ways, we each are constributing, but to read this mess takes a lot of time and breakdown, inside each operon, transposon, reporter gene, messenger gene, promoter, there are proteins from other organsims..............making models so as to use molecular and atomic particles..................into artificial life............... along the way natural selection, artificial selection will go on. We have to adapt.............. simple as that! When our native genes fight back, we have to be strong..............we are rejecting a lot of these artificial chromosomes, nuclei, and the phages are not responding. so we are rejecting both our native genes and some of the artificial( I believe vinyl? haven't gotten there yet), meanwhile the genes from other organisms, the enzymes, peptides, skin cells, insect stem cells, etc............are there, and yet we cannot identify, well hidden in the molecular machines................. or molecular processes.......... We are somewhere between artificial life and native original natural gene structures. bio/chem/geo/physics convergence..........don't want to get way ahead though! =========================== mini-Tn10 transposons: sounds like it is used as a genetic tool. coming up next. skytroll |
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