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Post by toni on Nov 10, 2009 10:49:21 GMT -5
hahah (sort of an important question): Kammy and Jeany and Ruth, where did your specimens (come from) on you? The ones that grew the Rhizopus that we all have pics of that look alike? Thanks. (maybe you've already said, and I'm sorry if I that missed it.)
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Post by fritolay66 on Nov 10, 2009 11:02:41 GMT -5
About Carnicom taking a petri and sampling the outdoor air. Here's my two cents on that. The outdoor petri is a control. When an outdoor sample is taken it is used as a comparison for the target petri. When a sample is taken from indoors from whatever, how would one tell what is the contaminate in the target petri? If the target petri and the control petri grow the same things, then the target petri is compromised and the environment in which the sample was taken is also compromised. It would indicate the environment is not capable of filtering what is outside. It is my understanding that what is different or the variance in the amount are indicative of the target identifier. Okay. I looked at my petri today and it would seem I have balls.....of fungus and hyphae and spheres. My dish look identical to what toni showed with her camera and the petri in her hand. It is a white fuzzy fungus with dark spots. It is so dense my microscope will not penetrate it for clear pictures towards the middle of the petri. All my samples were taken from the face area. Tear ducts, nose, plasma from pimple, and pimple skin. I also used an LED light to photog a couple of pics to show the bioluminecence of it. I have spheres. Then I have all of these, but I question whether these are just the agar or artifact. Then I have tons of hyphae with the spheres attatched to the hyphae. Just hyphae. And then I have these very dense spheres and hyphae. And then some weird spikey stuff. And my LED light pics. Hyphae Spheres Again, I kept this sample confined to my head area only. I am definately sending this in, as I want this damn fungus identified. My fuzz is also white, and I am also suspecting aspergillus. I was diagnosed with this in my ear when I was 19 and given chemotherapuetic antibiotic drug therapy for years. 9 years and many trips to the oncology unit. I am now about to get incredibly angry. Frito
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Post by toni on Nov 10, 2009 11:08:40 GMT -5
Oh goodness Frito!
It sure looks like the same thing as the rest of us-that cottony stuff with the spores.
The darker specimens reminds me of Sids specimens that she grew ( on page 1 ) in her petri dish.
Sid, did you ever get the "cottony growths"?
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Post by toni on Nov 10, 2009 11:17:03 GMT -5
Thanks also for the outdoor petri dish comment Frito.
I guess I'm just used to the breezes and winds kicking up here in Vegas, and with all the trees and shrubs and flowers in everyone's yards, and swimming pools and ponds, I get this mental image of (spores of everything) blowing in the air.
In fact we have a glass table outside, and I think about all the dust that gets all over it daily, and how much stuff really is in the air all the time.
And different seasons, that all might change too. Or depending on what the neighbors have planted in their yards.
It's just odd things that go through my head anymore (since Morgs) about all the fungus that's REALLY among us.
Heck the jet streams that blow over the planet are bringing/blowing in stuff from other countries too.
It's funny...never cared about that before, but it just feels like anymore that we're open doors to it all.
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Post by fritolay66 on Nov 10, 2009 11:36:26 GMT -5
Toni, I don't think your thinking is odd at all. Where as you put your thoughts down regularly, where some of us do not. Does not mean that it hasn't been thunk. Okay, now for some of my thinking. Whenever getting these black specks off of me, and I just found one last night on my nose. It moves of its own accord on my skin which is what attracts my attention to it in the first place, but when scoping, does not look like a parasite at any mag that I can see. Same with the white specks. Now, in throwing these in the toilet bowl, I have posted over and over again, that these things swim. This is not static discharge when hitting the water. Some will swim immediately, but then there are those in which will settle and then start to swim. I am telling you guys, my whatever swims. On the western blot, the only band that showed up for me is the 41 kDa band. Indicative of FLAGELLA. I do not remember whether aspergillus is flagellated, but I do remember flagellated fungus. !!! Frito
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vasue
Junior Member
Posts: 63
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Post by vasue on Nov 10, 2009 12:03:05 GMT -5
Agree with your outdoor petri dish comment, too, Frito. Agree also the black specks are live. Seems they can go into "inert" or "hiding" mode. Ever see a spider tuck itself into a ball when threatened? Suspect the same action here.
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Post by toni on Nov 10, 2009 12:23:53 GMT -5
Frito...me too ;D agree!
And YES, those black specks you're talking about, oh.... I'll tell you about my experience with them.
Every single morning ( AT 5 AM ) without fail...they'd wake me up!
I could feel my face tingling and tickling like someone poured ants on my face, and they were all scurring around.
I'd get up, look into the 10X mirror, and my face was COVERED in 100's of those tiny tiny tiny black specks.
They were no bigger than the periods at the end of these sentences.
I looked like someone had sprinkled fine ground black pepper on my face! Oh yes. (and this was every morning for almost an entire year this went on).
Everytime I tried to just get one of them...I mean JUST one, they'd all sink ( melt ) right back into my skin and be gone.
One day...I GOT ONE.
That is that black speck I'd taken pictures of (it looks like Bannany's black speck too).
It also looks like some type of mineral imho. Like magnetite or coal.
Have you seen those photos? I'll find one of them and post it.
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Post by toni on Nov 10, 2009 12:29:22 GMT -5
Oh, that was easy! I just googled (toni tiny black speck) Here's the link with pictures of this black speck (the one I did) catch. The others...they were as quick as can be, because everytime I tried to just get one off my face, it went right back into my skin. There's something real funny about these black specks, as at 5 am mine were set on some kind of timer. Every morning (for a year) these were tickling me at the same time, every day. Don't have a clue what can surface and sink into skin like these did (100's a day) and surface and cause all that tickling (at the same time every morning) but it is what was consistent and sure. Almost forgot, here's the link: lymebusters.proboards.com/index.cgi?board=rash&action=print&thread=7557And here's just a couple of the pictures from that link of both sides of the black speck next to a granule of salt so you can see how tiny these are. This was was actually larger, which is probably why I was ableto get it before it sunk back into my skin.
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Post by toni on Nov 10, 2009 13:26:56 GMT -5
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Post by jeany on Nov 10, 2009 13:56:02 GMT -5
YES, Toni...Rhizopus also infects the respiratory system. I was thinking about all the people who have problems with their sinuses, nose, ears and eyes. Coughing, and an increase of saliva and the sputum specimens we have seen. www.nlm.nih.gov/medlineplus/ency/article/000649.htmJeany
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Post by toni on Nov 10, 2009 17:54:30 GMT -5
Jeany,
I know...(the respiratory) oh...it's just incredible how many have "same sames".
Something in reading about molds too..
Is, our bodies will produce extra mucus to TRY and rid itself of a mold or fungal infection.
I'm wondering too if that's not what's happening. Now I did have that going on, but not anymore (after taking ginseng). So I sure don't know if that was for certain without a doubt the reason all that "slime stuff" cleared, but, I know my sister doesn't take ginseng, and she goes into gagging and throwing up daily convusions because she has it "that" bad.
Mr. Toni too, he had the "slime" syndrome really bad, and now he doesn't. So...I don't know, I only know what I'm seeing, and that's the ginseng seemed to do something in a good way. Now it could be coincidence, but there's just no way to be positive if that's what fixed that problem.
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Post by bannanny on Nov 11, 2009 3:43:20 GMT -5
Thanks for explaining everything toni... what you said about the outdoor mold/fungi thing just didn't compute right when I read it, but I understand now. I get confused so easy sometimes I just go blank... that being said, I have no idea what your take on it was that you explained frito! But I'm sure it was good! About what you put in your dish toni... I wonder if it takes putting a mix of different specimens in it like you did to get those results? Maybe it takes one thing reacting to another for the growth to begin? But then if you think it only grew from that one specimen within your lesion, what do you think I should use to get the same results being I don't have lesions? Have all you guys who seem to be growing what's possibly Rhizopus put several different specimens in your dish all at once too like toni did? Ok, I'm reading again... so frito, your dish had samples in it from your tear ducts, nose, plasma, and pimple skin. So you put more than one in yours too. By pimple, do you mean the red bumps in the skin? I do have those, and of course I have tear ducts, a nose, and plasma! Yours took longer to start producing tho right? That's kinda interesting... wonder if it takes digging into a lesion in order to see faster results? Just to say, I see alot of the black and white specs too frito... more in my environment than on me tho lately. When you see one pasted onto a window it makes it easier to see them too... which happened here. There were several, and being they were glued on a window I could see a long clearish white substance trailing out of every one of them. So maybe that's what makes them move? Whoah!! I just read what you said about the black specs sinking back under your skin toni, and along with what I just said to frito, they both gave me a vision (so to speak.) Your scope pic suddenly flashed in my mind... the one on page 5 of the strands with the "cherry balls" (and white ones too) attached. Do you think the black specs could be those "cherry balls" and the strands are what pulls them back under the skin... and are also what makes them move like yours did in the toilet frito? Ok, picturing that one just made one of my pics of a black spec enter my mind right along with it... It's got strands coming out of it too. So here's what I'm thinkin... I don't see why the strands wouldn't be what's moving the balls around or pulling them back under the skin either... possible maybe? Anyway, the reason I'm asking all the questions about what specimens you guys used is cuz mine doesn't seem to be doing much. I'm trying to figure out why, and no one's replied at all to my post last night about what the moisture in the dish might be from or anything about why the second I covered the dish, some of my sample went shooting up to the inside of the lid and attached to it. I took pics today, but they just aren't coming out very well. I'm gonna post them anyway tho. Maybe you can see enough to get an idea of what I'm saying... Ok, this 1st pic above might be a better pic than the one I posted before. When the gel I ooze gets real sticky, I can roll it out of my hands. Anyway, it's the only thing I put in my dish. I know it's blurry, but see all the moisture that collected on the inside of the lid? At this angle, at least you can see the pieces of the sample that shot up to the lid and attached... they turned white too, but the rest on the bottom of the dish stayed the creamy amber color. So maybe I shouldn't have even put the rolled out gel in my dish. No one else seems to have that problem anyway. I sure could use some help here with your ideas about it all. Guess I'll get another kit and put some hair from my head, nose, and even the goop in my ear in it and see what happens eh? Guess it's time I finally set my scope up too. One last question for kammy... what's your take now on the culture you did for me of the samples I sent you? Do you think it's something different than what's growing for these guys so far? I dunno myself. There's one thing I do know tho... I need to snap outta this place my mind's gone to somehow. I can't concentrate, I can't think, I feel like I'm floating around in space, and I hate it. I feel exhausted too, so much so my eyes burn cuz I can't sleep. I just feel like I'm not here ya know? I gotta get off this computer too... it took me 2+ hours just to write this one post. I'll see ya tomorrow if I can think...
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Post by fritolay66 on Nov 11, 2009 7:31:50 GMT -5
Nan,
Your sample is fine in my opinion. Keep the lid on it whether there is moisture from it or not. The petri is like one of those terrariums. When you put you skin or whatever is rolling off your hands, the moisture will disperse in the petri just like plants in the terrarium. That moisture will go away in a few days or sooner. Your sample looks as mine did so don't do anything to it for a few days. Also, keep that sample in the baggie it came with.
You are starting to worry me about how hard it is to concentrate and think. And then you have also been sick of late. If the causitive agent in which is making us so sick is mold, what are oing to help your body? Were you not seeing Dr. Hildy? Do you remember what helped you with that brain fog in the first place? And how are you detoxifying? You must detox dear friend. You must use binders so those toxins do not recirulate out into your body. Since cholestyroamine is prescription use the others more frequently.
Take care please.
Frito
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Post by fritolay66 on Nov 11, 2009 7:46:35 GMT -5
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Post by toni on Nov 11, 2009 9:11:02 GMT -5
Bannanny,
Your hyphae growth above (the white extending outwards) picture there? That looks like my hyphae too, how it looked when this Rhizo first took off growing.
It looked just like that. Like yours.
I had an idea about what you might want to try growing in a petri dish.
You know how you get the gel stuff from your hands?
Instead of rolling it out...
Could you maybe try this?
Gosh...not sure how to say it, it's one of those things easier seen than said.
When you feel that gooey on your fingers or hands, can you take like a clean piece of foil (tiny piece) like cut a piece off the edge of the foil, about 1" X 1"? (have that already cut) and placed in a clean zip lock so it doesn't get contaminated with anything else, and then it will be ready to go at anytime your hands feel that gooey stuff.
Then use that as a wipe, or a swipe on the "gel stuff".
Then place that 1" X 1" goo coated piece of foil into the petri dish?
Immediately put the lid back on, and set the dish where it will get indirect sunlight, like in a room on a dresser or something where it won't be disturbed. Have the curtains/blinds open, that way the sunshine will be indirect, (not right on it) but rather just the light in the room will be natural daytime lighting.
I was trying to think of something pretty sterile that you could swipe some goo/sticky onto and foil seems like a way to get a clean culture, and easily then placed into your dish.
The side of the foil that has the "goo" on it, or even a tad of sticky, is the side to place face down into the agar.
Or tear it in half, after the goo is on it, and place "one of the pieces face down", and the other piece face up, but, push the face up one down into the agar with your fingers, as to allow it to get the nutrients from the agar to grow.
I would think that would be better than rolling it off (maybe)?
Hope that will help.
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Post by toni on Nov 11, 2009 9:17:46 GMT -5
Bannanny, Me too, (again as Frito said) we're concerned about you of course. And we just want you feeling good. Are you taking Super B-complex with added B-12? I'm sorry if I sound like I'm being a "hen"...but I KNOW sweetie, that that foggy feeling can come when the body isn't getting it's B's. Me and Mr T awhile back (would watch the same movies) we'd already seen, (and realized we'd already) rented them before, but didn't remember them. The B's are for stress and the entire body really. Our body is under attack so it's stressed to the max with Morgs....and all. The body will tire easily in every facet without them. The body also utilizes them every 24 hrs. So one a day, keeps the foggies away. (that's our little saying) ;D
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Post by ruth on Nov 11, 2009 13:21:58 GMT -5
hi bannany, since you don't have lesions as we do, you must just produce the pre spores instead of the sclerotia (?) within the skin? just throwing that out.
so it would take longer to grow from just the biofilm.
mine grew the best sitting towards the back on the top of the refrigerator. i had it sitting in a little box without the lid just in case it needed light.
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Post by ruth on Nov 11, 2009 13:28:46 GMT -5
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Post by ruth on Nov 11, 2009 13:53:47 GMT -5
www.apsnet.org/phyto/SEARCH/1987/Phyto77_327.aspVIEW ARTICLE Ecology and Epidemiology Recovery of Fungi and Arthropods from Sclerotia of Sclerotinia sclerotiorum in Quebec Muck Soils. Osama Anas, Graduate student, Department of Plant Science, Macdonald College of McGill University, Ste-Anne-de-Bellevue, Quebec, Canada H9X 1C0; R. D. Reeleder, Assistant professor, Department of Plant Science, Macdonald College of McGill University, Ste-Anne-de-Bellevue, Quebec, Canada H9X 1C0. Phytopathology 77:327-331. Accepted for publication 3 July 1986. Copyright 1987 The American Phytopathological Society. DOI: 10.1094/Phyto-77-327. Baiting samples of muck soil from Quebec with sclerotia of Sclerotinia sclerotiorum revealed that Trichoderma, Gliocladium, Penicillium, Sporidesmium, Rhizopus, Myxomycetes, Bradysia (dark-winged fungus gnat), and Onychiurus sp. (springtails. Order Collembola) were present in the soils. The number of larvae of Bradysia was positively correlated with low soil pH, high levels of organic matter, and high levels of nitrate in the soil. There was no correlation between fungi or Onychiurus sp. recovered and any of the above soil parameters. In in vitro tests, sclerotia damaged by the feeding of the larvae of Bradysia had levels of mycelial germination of 0–30%, whereas undamaged sclerotia germinated at a rate of 95%. When sclerotia were buried at different depths in soil, and larvae or adults of Bradysia were placed on the soil surface, predation of sclerotia was greatest in the top 2 cm of soil. The larvae were recovered from as deep as 9 cm in the soil. the rhizopus brings in the (thrips, for me) collembola, and fungus gnats as well as those parasitic wasps i use to get.
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Post by Sidney on Nov 11, 2009 18:16:26 GMT -5
Ruth said:
the rhizopus brings in the (thrips, for me) collembola, and fungus gnats as well as those parasitic wasps i use to get.
First hand knowledge of Collembola, fungus gnats and Hymenoptera for me, to name only a few critters.....
Thanks, Ruth, and all. Great work.
Personally, I feel like an old rag doll someone ripped apart and removed all the stuffing. I think you know what I mean.
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