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Post by lilsissy on Sept 4, 2009 1:25:36 GMT -5
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Post by lilsissy on Sept 4, 2009 1:31:49 GMT -5
hmmmm.......... the zinc finger protein was what SilentSuperBug talked about and also insect no developement no embro, Crook NE, Clem RJ, Miller LK An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif. J Virol. 1993; 67: 2168-74 Display abstract Spodoptera frugiperda SF-21 cells infected with Autographa californica nuclear polyhedrosis virus mutants which lack a functional p35 gene undergo apoptosis, a type of programmed cell death. To identify p35-homologous genes in other baculoviruses, A. californica nuclear polyhedrosis virus DNA containing a deletion in p35 was cotransfected into SF-21 cells along with genomic DNAs from other baculoviruses. One of the viral DNAs which were able to rescue wild-type infection was from Cydia pomonella granulosis virus (CpGV). The CpGV gene responsible for the effect was mapped to a 1.6-kb SalI-SstI subclone of the SalI B fragment of CpGV. The sequence of the SalI-SstI subclone revealed an open reading frame capable of encoding a polypeptide of 31 kDa which was sufficient to rescue wild-type infection; this gene was thus called iap (inhibitor of apoptosis). The predicted sequence of the IAP polypeptide exhibited no significant homology to P35 but contained a zinc finger-like motif which is also found in other genes with the potential to regulate apoptosis, including several mammalian proto-oncogenes and two insect genes involved in embryonic development. In the context of the viral genome, both iap and p35 were able to block apoptosis induced by actinomycin D, indicating that these genes act by blocking cellular apoptosis rather than by preventing viral stimulation of apoptosis. Several independent recombinant viruses derived from cotransfections with either the entire CpGV genome or the 1.6-kb subclone were characterized. So what do these Spodoptera frugiperda SF-21 cells look like, tinyurl.com/mtf6e5tinyurl.com/n4anq5 |
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Post by lilsissy on Sept 4, 2009 1:43:53 GMT -5
Amazing work ladies,
Jen
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Post by jeany on Sept 4, 2009 4:24:43 GMT -5
Great research, Jen!
Thank you for participating!
Jeany
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Post by kammy on Sept 4, 2009 9:34:35 GMT -5
you are not going to believe this crap The picture from the patio looked like neural cells , quite a bit like them that was freaky I thought and just for the heck of it I goggled baculovirus and neural cells. well now look at this, www.freepatentsonline.com/y2009/0055941.htmlHey Sissy... You mean 'Barb's thread'...? is that what you're referring to? (from the patio?... not sure) I'm still reading and waking up on the research you've brought in here... what's a neural cell? Yes, and ty for your help and support... |
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Post by kammy on Sept 4, 2009 9:46:36 GMT -5
Neural Cell I see where neuroscience is a field all of its own. Look at all the different sciences we have touched looking at this disease, gosh! When I enter it in Wikipedia, it's not there, it gives a reference to neuron cell or nerve cell... en.wikipedia.org/wiki/Nerve_cell"A neuron (pronounced /ˈnjʊərɒn/ N(Y)OOR-on, also known as a neurone or nerve cell) is an excitable cell in the nervous system that processes and transmits information by electrochemical signalling. Neurons are the core components of the brain, the vertebrate spinal cord, the invertebrate ventral nerve cord, and the peripheral nerves." I think I see what you're saying, correct me, if I'm wrong? Here's a photo from a human lesion culture: Sissy - You're saying they have 'programmed' this fungus/capsid network to preform similar to human neuron cells? |
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Post by lilsissy on Sept 4, 2009 23:26:30 GMT -5
Kaamy I just noticed a strong similarity between the two,
remember the it patent , I believe they Can transfer many types of cells and organisms this way and may have used the baculovirus to deliver D.N.A. modification to us as well as to the insects which they have experimented with.
Some chem trail analysis has shown blood cells , I am not referring to red rain either.
Baculovirus I am convinced is big big part of this.
Joesj pics match yours to me anyway .
I also watched as Chaos my sister picked a embedded fly type organism from her thumb.
Then I see your pictures
Yes I too believe Silentsuper bug says
Insect technology
Development with no embryo.
also he wrote me that anabuse workes on the zinc finger protein
Too much the same evidence to be a co-incidence to me.
Now did you notice from my above reply,
Spodoptera frugiperda SF21 cells infected with Autographa californica nuclear polyhedrosis virus mutants which lack a functional p35 gene undergo apoptosis, a type of programmed cell death. To identify p35-homologous genes in other baculoviruses, A. californica nuclear polyhedrosis virus DNA containing a deletion in p35 was cotransfected into SF-21 cells along with genomic DNAs from other baculoviruses. One of the viral DNAs which were able to rescue wild-type infection was from Cydia pomonella granulosis virus (CpGV). The CpGV gene responsible for the effect was mapped to a 1.6-kb SalI-SstI subclone of the SalI B fragment of CpGV. The sequence of the SalI-SstI subclone revealed an open reading frame capable of encoding a polypeptide of 31 kDa which was sufficient to rescue wild-type infection; this gene was thus called iap (inhibitor of apoptosis). The predicted sequence of the IAP polypeptide exhibited no significant homology to P35 but contained a zinc finger-like motif which is also found in other genes with the potential to regulate apoptosis, including several mammalian proto-oncogenes and two insect genes involved in embryonic development. In the context of the viral genome, both iap and p35 were able to block apoptosis induced by actinomycin D, indicating that these genes act by blocking cellular apoptosis rather than by preventing viral stimulation of apoptosis. Several independent recombinant viruses derived from cotransfections with either the entire CpGV genome or the 1.6-kb subclone were characterized.
I really think we should try to narrow in on this clip for clues.
Maybe they incorporated cyanobacteria was it he called it(SSB)
This chit whatever it is loves keratin, I believe it crosslinks to it or in other words,
tranmutes to it or in other words is able to weave itself in it or in other works STACKS IN IT does anyone remember the stacking circles found ,was it by mark darrah?
Those stacks are found it the hair of dead people B.T.W.
Putting everyones work together sometimes shines a light !
I think thAT MAYBE A LIGHT HARVESTING ABILITY BY HUMAN CELLS is one of the entended outcomes.
Tanshuman are changed by various organisms but your baculoirus is great for accomplishing that and so is Agrobacterium.
jen
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Post by kammy on Sept 5, 2009 2:25:24 GMT -5
Yes Jen, I think the Neural Cell - the way it works and looks, is very similar to what we're seeing, that 'bulb' looks like our 'infection bulb' and the way it branches... they usually take their inventions from something that is known.
I think we already had the SF21 involved? I wonder why they call it the "wild type" and "mutants" of the ACNPV, I probably have those... lol - What's a zinc finger protein - you lost me there. I don't understand what "apoptosis" (cell death) has to do with Morgellons, none of my BV are dying very quickly. I slightly understand it - they had something missing, so they infected the next batch to make up for it with a virus and two more insect genes that created a new genome that somehow affects cell death? Translation please?
Agrobacterium hasn't been ruled out as a factor. I see another factor that's a part of it, I just don't know what I'm looking at, yet, what the official name of it is? Maybe, I can bring it out and we can figure it out? I'm just saying that this baculovirus 'system' is around which Morgellons spins... and I would think a lot of different things can come in and enter the system and then be a consistent part of it, making all of us a little different with our symptoms, etc.
As far as everyone with Morgellons having fungus gnats? I think that if you have or had lesions, the specks, a biofilm, and fibers that you have some sort of small fly or parasitic wasp infestation. We're not sure what role the parasitic wasp plays in Morgellons but it is highly suspect to be involved. We've learned that through HGT the wasp picks up something from every insect it comes into contact with, creating a large number of variables.
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Post by Jill on Sept 5, 2009 6:37:28 GMT -5
Yes Jen, I think the Neural Cell - the way it works and looks, is very similar to what we're seeing, that 'bulb' looks like our 'infection bulb' and the way it branches... they usually take their inventions from something that is known. I think we already had the SF21 involved? I wonder why they call it the "wild type" and "mutants" of the ACNPV, I probably have those... lol - What's a zinc finger protein - you lost me there. I don't understand what "apoptosis" (cell death) has to do with Morgellons, none of my BV are dying very quickly. I slightly understand it - they had something missing, so they infected the next batch to make up for it with a virus and two more insect genes that created a new genome that somehow affects cell death? Translation please?Agrobacterium hasn't been ruled out as a factor. I see another factor that's a part of it, I just don't know what I'm looking at, yet, what the official name of it is? Maybe, I can bring it out and we can figure it out? I'm just saying that this baculovirus 'system' is around which Morgellons spins... and I would think a lot of different things can come in and enter the system and then be a consistent part of it, making all of us a little different with our symptoms, etc. As far as everyone with Morgellons having fungus gnats? I think that if you have or had lesions, the specks, a biofilm, and fibers that you have some sort of small fly or parasitic wasp infestation. We're not sure what role the parasitic wasp plays in Morgellons but it is highly suspect to be involved. We've learned that through HGT the wasp picks up something from every insect it comes into contact with, creating a large number of variables. In my research on T parva- the Zinc finger proteins come up frequently- obviously key to the re-engineering of DNA per below: en.wikipedia.org/wiki/Zinc_fingerFair use Excerpt: Zinc fingers are small protein domains that can coordinate one or more zinc ions to help stabilize their folds. They can be classified into several different structural families and typically function as interaction modules that bind DNA, RNA, proteins or small molecules. The name "zinc finger" was coined to describe the hypothesized structure of the repeated unit in Xenopus laevis transcription factor IIIA.www.zincfingers.org/default2.htmFair use Excerpt: Cys2His2 zinc fingers (“zinc fingers”) define the most common transcription factor family in organisms ranging from yeast to humans. “Designer” zinc finger proteins with purposefully re-engineered DNA-binding specificities provide a powerful and broadly applicable technology for targeting functional domains to essentially ANY gene of interest in virtually ANY cell type. Zinc finger nucleases (ZFNs) are an extremely powerful tool for performing targeted genomic manipulation in a variety of cell types including plants, insects, and humans. ZFNs consist of an engineered DNA-binding zinc finger domain linked to a non-specific endonuclease domain and can introduce double-stranded breaks (DSBs) that stimulate both homologous and non-homologous recombination, processes that can be harnessed to perform genomic manipulation. The capability to alter any genomic locus of interest will have tremendous potential in both research and gene therapy applications. However, the general application of this enormously promising technology depends critically on the ability to design zinc finger domains targeted to any genomic locus of interest. ** Cell death- apoptosis - is at the heart of the process- of what is being called Morgellons. |
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Post by Jill on Sept 5, 2009 6:52:52 GMT -5
www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=19055776BMC Genomics. 2008; 9: 582. Published online 2008 December 3. doi: 10.1186/1471-2164-9-582. PMCID: PMC2612703 Copyright © 2008 Guo and Silva; licensee BioMed Central Ltd. Properties of non-coding DNA and identification of putative cis-regulatory elements in Theileria parva Xiang Guocorresponding author1,2 and Joana C Silvacorresponding author1,3,4 1The Institute for Genomic Research/J. Craig Venter Institute, 9712 Medical Center Drive, Rockville, MD 20850, USA 2Advanced Biomedical Computing Center, NCI-Frederick/SAIC-Frederick Inc., Frederick, MD 21702, USA 3Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA 4Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA corresponding authorCorresponding author. Xiang Guo: guo424@gmail.com; Joana C Silva: jcsilva@som.umaryland.edu Received July 4, 2008; Accepted December 3, 2008. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fair use Excerpt: Next, we investigated the biological relevance for each motif in the context of the function of adjacent genes. A hypergeometric test demonstrated that certain functional categories are significantly enriched among genes downstream of motifs 1 and 3 (p < 0.01), but not of motif 2. Motif 1 is associated with genes involved in protein synthesis, with telomeric ORFs, and with proteins containing signal peptides, while motif 3 is associated with genes related to protein fate (Additional file 1). Using STAMP [23], we identified known motifs that are most similar to each of the three Theileria motifs. Motif 1 has similarity to a DNA consensus binding site for myeloid zinc finger protein 1 (MZF1), a C2H2 zinc finger transcription factor involved in granulopoiesis,
cellular proliferation and oncogenesis [24] (Figure (Figure4).4Figure 4).
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Post by Jill on Sept 5, 2009 6:55:57 GMT -5
Note from the above link- Granulopoiesis - last sentence: en.wikipedia.org/wiki/GranulopoiesisFair use Excerpt: Granulopoiesis
Granulopoiesis (or granulocytopoiesis) is hematopoiesis of granulocytes.
It occurs primarily within bone marrow and involves the following stages: * Pluripotential hemopoietic stem cell * Myeloblast * Promyelocyte * Eosino/neutro/basophilic myelocyte * Metamyelocyte * Band cell (Stab cell) * Granulocytes (Eosino/neutro/basophil) It can be stimulated by Candida albicans.[1] ** en.wikipedia.org/wiki/GranulocyteFair use Excerpt: Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm.[1] They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes,[2] the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins. |
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Post by Jill on Sept 5, 2009 7:19:20 GMT -5
Theileria parva is a genus of Protozoan parasite - family theileriidae. Transmitted by Ticks and they multiply in the leukocytes and then they invade the erythrocytes. Leukocytes- White Blood Cells WBC en.wikipedia.org/wiki/White_blood_cell Erythrocytes= Red Blood Cells RBC www.ndsu.nodak.edu/instruct/tcolvill/435/erythrocytes.htm The tick that transmits the T parva, creates what is termed a Cement Cone or Schizont on the host. This cone or schizont as described - various sources- sounds exactly like the callus described by Cliff Mickelson and that described by many sufferers on various boards. The cone with 2 layers to include a cortex, is very difficult to remove from the skin of the host. The Baculovirus- used in Vaccines for T parva: www.faqs.org/patents/app/20080299148 The fact that T Parva- mimics Leukemia- is key here as is the fact that the blood is modified. |
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Post by Jill on Sept 5, 2009 7:26:58 GMT -5
From what I've read and researched, it is my belief that the Theileria parva that Robert Smith ID'd is key in Morgellons. The cause. Start on page 4- link below- and find the references to T parva: tinyurl.com/n2ztq5 Most likely, the ID'd T parva is sourced from vaccines. Cattle, dogs and other animals are commonly vaccinated against the disease. The vaccine could transfer other components used in the process (of making the vaccine)- such as the Baculovirus. |
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Post by Jill on Sept 5, 2009 7:51:12 GMT -5
Re: Insects tinyurl.com/nhywbjFusion to green fluorescent protein improves expression levels of Theileria parva sporozoite surface antigen p67 in insect cells |
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Post by Jill on Sept 5, 2009 8:18:05 GMT -5
www.biologynews.net/archives/2005/07/02/usafrican_project_deciphers_deadly_parasite_genome.htmlFair use Excerpt: Nobel Prize winning German microbiologist Robert Koch, who had been sent to east Africa to study the disease, was the first to describe the T. parva parasite and made the first attempt to develop a vaccine. Veterinary bacteriologist Arnold Theiler later made significant discoveries about the parasite, which is named for him. Vish Nene, a former ILRI scientist who came to TIGR in 2001 to join the T. parva project, says the genome sequence provides useful information that will help scientists better understand how the parasite malignantly transforms cattle white blood cells into cancer-like cells that multiply rapidly, eventually leading to fever and death. By studying that process, scientists are trying to learn more about the genesis of human cancers.
"This parasite has an astonishing ability to induce cancer in its host cell in a way that is reversible," says Nene. "There are clear links to cancer biology in humans, and this study has given us some clues to pursue." ** Because the process- of inducing Cancer- is reversible- it seems it would behoove us to pursue that information. Could be the CURE.
Clearly- T parva is a part of the Morgellons condition if not the Cause (which I believe to be true). [/size] Jill How many posters have died of Cancer ? Have Cancer now ?
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Post by kammy on Sept 5, 2009 11:13:48 GMT -5
Where should this thread go from here?
TY, Jill, Jen... there's some brilliant minds here, I hope the folks appreciate what it is you're trying to bring and explain to them? Finally, a coming together of like-minded intelligence, we're making progress. We haven't had this before, our best minds have been scattered about, working on this and that, not really knowing for sure what direction to be looking in.
My research is done for now. The way I look at research is... I can speculate that Morgellons is this or that and odds are with all that is appearing to be involved - I might be correct - but, I ask myself - HOW am I going to show or prove that this is MOST likely to be happening?
We have a scientist that's coming in here shortly to look at this, they've already been down 25 different paths as to where to look, I'm sure they're already overwhelmed? What do we want them to concentrate on when they start their research?
I think we need to concentrate on the "major players" - the things we can see, that are obvious. If we can identify these 'obvious unknowns' for them using our gifted minds, and there's some smart people here, this will help us put the Morgellons puzzle together a whole lot quicker?
The thing is... we've got to look at the same 'picture' in order to critique, study, or figure it out. In other words, be somewhat on the same page, but not necessarily in total agreement, that shows or finds the holes in the theory. We can speculate, but we need to use solid science based on good hypothesis, not to say that you're not, gals. I have watched people go in many different directions and in circles with their research, I've done the same, but I always knew what I was looking for.
I knew that when the objects I was presenting matched the "Silent Super Bug" movie back on the "Crypto Thread"... that I was on base. And, I also knew that most of you thought I was presenting something that was exclusively something that was personal to me. That what I was showing you was something I had... and didn't pertain to you?
And, you see how long it took me to figure the main part of it out, now, if I had a background in molecular biology, it shouldn't have taken me very long at all! These debris particles coming out of our lesions are cells - put them in a Petri Dish and look, how hard is that? A very simple technique and you don't have to boil, or spin... etc. anything, I don't understand the hold up in getting the word to our doctors and us getting some treatment - at all! 'It's just a small fly, it won't kill them quickly...' well, don't they know our flies can hatch and go over to their granddaughter's picnic table and land on her chicken and hello?... she's got this? What kind of mentality is this that is allowing us to go around seeding these flies until the whole planet becomes infected before they do anything about it, before it's too late?
Let's say this upcoming CDC report discloses that we have a GM insect infestation? You see that word, "GM"... oh yes... what's going on in that world - it's partially the politics behind why we're being ignored? One of many reasons?... we'd hate to slow down those GM companies...
I don't know if our Health Organizations can come out and say what's wrong with us without creating some sort of world-wide panic and that's their fear? The politics behind everything, it seems, is based on economics... what happened to ethics - what's right and what's wrong, and for the best interest of man and the planet?
I'm not discounting your theories, either of you, I'm glad you're here. I'm just asking you, how do they fit with what we know? How do you want to present them to a scientist that might look our way? How long is it going to take a scientist to find the cancer part of the baculovirus system, acquire the demographics of how many of us and how quickly cancer is developing inside our disease, and when they do - what are they going to be looking at with the main system to stop it and by the time they've figured that out... the whole world is covered in gnat/fly seeds?
What information can we bring the people that they can take to their doctors to educate them about what's happened to us, places for the doctors to start looking in order to verify our beliefs? Yes, go out and print the CDC's and whoever else's web page to take your doctors... you'll still be at square one.
I'm asking you - Jen, Jill, Jeany (the 3 J's) that are participating here... and our other great minds... what do we do next, where do we go from here?
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Post by kammy on Sept 5, 2009 11:59:25 GMT -5
From Jen above:
"Development with no embryo.
also he wrote me that anabuse workes on the zinc finger protein"
Who wrote this?
I've seen a reference to where anabuse might or helps with Morgellons. What do you know about this, Jen?
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Post by kammy on Sept 5, 2009 12:03:48 GMT -5
Re: Insects tinyurl.com/nhywbjFusion to green fluorescent protein improves expression levels of Theileria parva sporozoite surface antigen p67 in insect cells Ok, Jill, if T parva is a part of Morgellons, how can the people test for this? That would give us a tick connection in common to Lymes? I wonder if Lymes looks microscopically similar to Morgellons in any way, in other words, if we both have a baculovirus system happening within our diseases? |
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Post by kammy on Sept 5, 2009 12:42:02 GMT -5
I know most don't understand the complete, complex life cycle of our particular baculovirus yet. I'm working on it, (just in case - it's there in my writings somewhere).
So, by what we're seeing, that the gnat is contaminating the water it touches, then why aren't larger numbers of people showing evident Morgellons symptoms? Jo says it can take up to one year for a fungus gnat to hatch and that any treatment we seek should be for at least a year's period and she's probably right.
I'm not sure how long the baculovirus can lay dormat in nature or inside the human body before it decides the time is right to start its processes, probably years? This is a very silent, "superbug", they chose the right name, didn't they? From my research, it seems to me that more and more cases of Morgellons should be evident in the near future. We suspect there is a dormancy period.
This dormancy period might depend on how you come into contact with the baculovirus - in what stage of it? For instance, we know for a fact that if we ingest a fly egg... we're going to get sick and it probably won't take long to feel some of these symptoms. If we inhale a baculovirus speck, or drink water with the frass/fibers in it, this might take longer to show up or these may not be ways to contract Morgellons at all? ... we just don't know these answers yet. It is most likely that ingesting one of the adult's eggs is a definite.
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Post by toni on Sept 5, 2009 12:44:45 GMT -5
Kmarie said:
I certainly have NO CLUE, but we do KNOW that the LYME/Borrelia Spirochete HAS definately been "manipulated". In Becky's video, she did mentioned making microbe "mixes" with different viruses. (i.e. baculovirus, and the T. Parva) .... So it could be VERY possible that the reason some/most are testing positive for Lyme/borrelia is because of we have part of it.
Toni says: I like that thought...as it seems like a very good "possibility". Very interesting for sure!
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